全文获取类型
收费全文 | 18244篇 |
免费 | 1067篇 |
国内免费 | 1707篇 |
专业分类
林业 | 925篇 |
农学 | 1455篇 |
基础科学 | 139篇 |
886篇 | |
综合类 | 5706篇 |
农作物 | 1302篇 |
水产渔业 | 788篇 |
畜牧兽医 | 5278篇 |
园艺 | 3695篇 |
植物保护 | 844篇 |
出版年
2024年 | 63篇 |
2023年 | 246篇 |
2022年 | 588篇 |
2021年 | 702篇 |
2020年 | 727篇 |
2019年 | 911篇 |
2018年 | 635篇 |
2017年 | 825篇 |
2016年 | 1090篇 |
2015年 | 1041篇 |
2014年 | 1109篇 |
2013年 | 1101篇 |
2012年 | 1424篇 |
2011年 | 1499篇 |
2010年 | 1118篇 |
2009年 | 1116篇 |
2008年 | 1047篇 |
2007年 | 1240篇 |
2006年 | 862篇 |
2005年 | 729篇 |
2004年 | 563篇 |
2003年 | 451篇 |
2002年 | 308篇 |
2001年 | 258篇 |
2000年 | 256篇 |
1999年 | 174篇 |
1998年 | 130篇 |
1997年 | 110篇 |
1996年 | 105篇 |
1995年 | 70篇 |
1994年 | 77篇 |
1993年 | 84篇 |
1992年 | 46篇 |
1991年 | 36篇 |
1990年 | 32篇 |
1989年 | 42篇 |
1988年 | 36篇 |
1987年 | 28篇 |
1986年 | 23篇 |
1985年 | 14篇 |
1984年 | 11篇 |
1983年 | 7篇 |
1982年 | 5篇 |
1981年 | 21篇 |
1980年 | 11篇 |
1979年 | 5篇 |
1976年 | 4篇 |
1962年 | 6篇 |
1956年 | 18篇 |
1955年 | 5篇 |
排序方式: 共有10000条查询结果,搜索用时 452 毫秒
81.
AIM:To explore the effects of oxidized low density lipoprotein (OxLDL) and one of its component— lysophosphatidylcholine (LPC) on cholesterol efflux from mouse macrophage foam cells.METHODS:(1) Cholesterol efflux induced by apoAI from mouse peritoneal macrophage foam cells loaded with OxLDL or acylated LDL(AcLDL) was measured. (2) Cholesterol efflux induced by LPC and apoAI from macrophage foam cells separated from normal or apoE gene deficient (E0) mouse loaded with AcLDL were measured.RESULTS:(1) When the macrophage foam cells were incubated with apoAI, cholesterol efflux from AcLDL-induced macrophage foam cells increased significantly compared to that of OxLDL-induced macrophage foam cells. (2) LPC promoted cholesterol efflux from macrophage foam cells in relation to both dosage and time. When LPC was incubated with E0 mouse macrophage foam cells, the released cholesterol mass was significantly lower than that of normal mouse macrophage foam cells. It was also found that cholesterol efflux induced by apoAI normally occurred in E0 mouse macrophage foam cells.CONCLUSIONS:(1) OxLDL accumulated cholesterol in macrophages and impair cholesterol efflux. (2) LPC induced cholesterol efflux from macrophage foam cells, which may occur via apoE pathway. 相似文献
82.
AIM:To explore transdifferentiation potential of Sca-1+ cells from murine fetal liver. METHODS:2×103 of Sca-1+ cells from male murine fetal liver were transfused into female mouse irradiated lethally with γ ray from 60 Co source (10 Gy) via tail vein. Two months later, FISH and immunohistochemistry were used to detect the situation for transdifferentiating of the donor cells (male cells) in tissues of female recipient mouse. RESULTS:The renal tubular epitheliocyte-like and neurocyte-like cells with Y chromosome were found on the sections of renal and brain tissues from female recipient mice. These cells have phenotype characteristics of RCA+/CD45-F4/80- and NueN+/CD45-F4/80-, respectively. CONCLUSION:The evidence is provided for Sca-1+ cells from murine fetal liver to transdifferentiate into both renal and brain tissue cells. 相似文献
83.
AIM:To investigate therapeutic effects of recombinant human growth hormone(rhGH) on rat sepsis and its possible mechanisms.METHODS:Mean arterial pressure (MAP), levels of plasma TNFα, IL-1β and endotoxin, leukocyte count and survival rate within 1 week were determined after E. coli injection among control group, sepsis group and sepsis+rhGH group.RESULTS:(1)rhGH diminished the decrease of MAP, reduced plasma endotoxin and TNFα levels and increased neutrophil ratio in total leukocytes in sepsis rat. rhGH increased survival rate within 1 week on sepsis rat. (2)No changes were found in IL-1β level among the three groups.CONCLUSION:rhGH showed desirable beneficial effects on rat sepsis, which may attribute to: improving circulatory function;maintaining intestinal mucosa barrier, attenuating bacteria/endotoxin translocation and inhibiting the production and release of TNFα. 相似文献
84.
LING Yun-biao XU Rui-yun QIU Wan-shou CHU Zhong-hua YANG Hong-zhi TAN Zhi OU Qing-jia 《园艺学报》2003,19(12):1649-1652
AIM: To investigate the effect of Chinese herbs, Ganxianfang(GXF), on rat hepatic stellate cells (HSC) proliferation and collagen synthesis. METHODS: Two types of herb serum, portal venous serum and circumferential venous serum, were prepared from rats infused intragastrically with 16, 8, 4 times adult dose of GXF decoction. HSC isolated from rat liver were processed with the above sera in vitro. Then we mensurated the radioactivity of HSC admixed with [[3H]H]proline and [[3H]H]thymine to judge the effect on proliferation and collagen synthesis of HSC. RESULTS: Both two types of serum collected 0.5, 1, 2 h after intragastrical infusion inhibited HSC proliferation (P<0.05), and the serum collected 1 h after intragastrical infusion had the strongest effect (P<0.05). Portal serum decreasea collagen synthesis (P<0.05), but circumferential serum had no effect (P>0.05). CONCLUSION: Inhibition of HSC proliferation and decrease of collagen synthesis may contribute to the GXF antifibrotic action. 相似文献
85.
AIM:To explore the serum levels of certain adhesion molecules and its significance in acute coronary syndrome(ACS). METHODS:The subjects included 40 patients with acute myocardial infarction(AMI) and 40 patients with unstable angina pectoris (UAP). Among the 80 patients, 60 patients accepted a follow-up 4 months. At the same time we selected 40 controls from people who attended a routine health check in the university. Serum levels of E-selectin, sICAM-1, sVCAM-1 were measured by ELISA.RESULTS:Serum levels of E-selectin, sICAM-1, sVCAM-1 were significantly higher in the ACS group(AMI or UAP) than in the control group. Four months later, the levels of E-selectin, sICAM-1 became significantly lower in the follow-up group than in the ACS group, while sVCAM-1 showed no significant difference. CONCLUSION:Serum levels of E-selectin, sICAM-1 may have certain diagnostic value for ACS, and can be a useful marker reflecting the stability of the disease. 相似文献
86.
87.
水稻与稻瘟病菌非亲和性互作中重要防御酶活性变化规律研究 总被引:14,自引:4,他引:14
选以CO39为背景的水稻抗稻瘟病近等基因系,与稻瘟菌生理小种ZC13(菌株97-151a)组成的3类典型非亲和性互作,以亲和性互作为对照,对各互作中过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)、几丁质酶及β-1,3-葡聚糖酶的活性变化规律进行了系统研究。完全非亲和性互作C101A51/97-151a、高度非亲和性互作C101L AC/97-151a及中度非亲和性互作C104 PKT/97-151a,POD比活性接种后即开始明显升高,48h前达到高峰,升高趋势一直持续到7d完全显症时,幅度基本与各互作非亲和程度呈正相关;亲和性互作CO39/97-151a接种后40 h POD比活性才开始升高,4~6 d达到高峰,峰值也较大。3类非亲和性互作PAL比活性在接种后0 h或16 h开始较明显升高,整个互作中形成3~4个较明显的峰;亲和性互作中PAL比活性一直明显下降。3类非亲和性互作外切几丁质酶比活性接种后即开始升高,基本一直保持升高趋势,在40 h前幅度较大,并形成1~3个较高的峰;亲和性互作外切几丁质酶比活性接种后即开始大幅度升高直至完全显症,48h后幅度远高于非亲和性互作。3类非亲和性互作β-1,3-葡聚糖酶比活性在24 h内开始较明显升高,在48h前形成2~3个较明显的峰;亲和性互作在接种后β-1,3-葡聚糖酶比活性即开始升高,在48h后显著高于非亲和性互作。讨论了POD、PAL、几丁质酶及β-1,3-葡聚糖酶参与水稻抗稻瘟病的可能性。 相似文献
88.
月季切花乙烯受体ETR1 cDNA克隆及其序列分析* 总被引:6,自引:0,他引:6
根据乙烯受体基因ETR1 保守区设计引物, 分别以瓶插寿命差异显著的月季切花品种‘德克萨斯’和‘维亚蒂’为材料, 通过RT-PCR 从花瓣中扩增出了797 bp 的cDNA 片段, 它编码265 个氨基酸。测序和序列分析表明,‘德克萨斯’重组质粒中插入片段的核苷酸序列之间完全相同, 命名为pRT-ETR1。而‘维亚蒂’所获得的重组质粒中插入片段的核苷酸和氨基酸序列之间存在差异, 同源性分别为85. 2 %和92. 1 % , 分别命名为pRV-ETR1-V4 和pRV-ETR1-V5。pRV2 ETR12V5 的核苷酸和氨基酸序列与‘德克萨斯’pRT2 ETR1 的同源性均达99 %以上; pRV-ETR1-V4 中的核苷酸和氨基酸序列与‘德克萨斯’pRT-ETR1 的同源性分别为85. 0 %和92. 5 %。上述插入片段与桃、苹果、天竺葵、拟南芥等植物的ETR1相应区域高度同源, 其氨基酸同源性均大于90 %。 相似文献
89.
90.