利用EPG技术分析西花蓟马在4种寄主植物上的取食行为

采用直流刺吸电位仪EPG(direct current electrical penetration graph, EPG)技术分析西花蓟马Frankliniella occidentalis雌雄虫在4种寄主植物上的取食行为,探讨西花蓟马雌雄虫取食行为差异及与寄主适合性的相关性。结果表明: (1)西花蓟马在辣椒上的非取食刺探次数显著少于其他寄主植物。雌虫在辣椒、番茄、曼陀罗和矮牵牛上的非取食刺探分别为2311,3289,3610和3044次,雄虫在辣椒、番茄、曼陀罗和矮牵牛上的非取食刺探分别为1527,2772,2214和1968次。西花蓟马在矮牵牛上的短取食刺探显著高于番茄,雌虫在番茄和矮牵牛上的短取食分别为688和1444次,雄虫在番茄和矮牵牛上的短取食分别为159和809次。西花蓟马在矮牵牛上的长取食刺探次数少于在其他寄主植物。(2)雌虫比雄虫产生更多的非取食刺探和短取食刺探,雄虫的长取食刺探显著高于雌虫。在矮牵牛上,雌虫的非取食刺探次数和非取食刺探总时间为雄虫的15倍。在番茄上,雌虫的短取食次数和短取食总时间分别为雄虫的4倍和2倍;雄虫的长取食次数和长取食总时间分别为雌虫的2倍和7倍。由此可见,西花蓟马的取食行为与寄主适宜性之间无显著的相关性。     

地面弥雾机与六旋翼植保无人机在芒果冠层中雾滴沉积性能对比

为解决芒果园传统植保作业中农药用量大、施药不均匀、作业效率低等问题,并构建智慧芒果园,本研究对比了地面弥雾机和六旋翼植保无人机两种果园施药机具在芒果冠层中的药液雾滴沉积性能。将芒果冠层分为上中下层,以柠檬黄为示踪剂,使用高清相纸与滤纸采集药液雾滴,通过图像处理等手段分析雾滴沉积分布均匀性。试验结果表明,植保无人机在芒果树上部冠层叶片表面的雾滴覆盖率显著高于地面弥雾机,在其余冠层部位,两种施药机具在叶片表面药液无显著差异覆盖;植保无人机处理组叶片正反面平均覆盖率均为地面弥雾机的1.5~2倍,对叶片背面的防治优于地面弥雾机。地面弥雾机处理组叶片正面雾滴密度显著高于植保无人机,叶片背面无显著差异,植保无人机处理组正反面均未满足低量喷雾20个/cm2的病虫害防治要求。地面弥雾机药液沉积集中在中下冠层（61.1%）,植保无人机集中在上部冠层（43.0%）,冠层内部沉积比例地面弥雾机（48.6%）＞植保无人机（25.5%）,但地面弥雾机在冠层上部沉积能力不足,沉积占比仅为17%。研究表明,相较于植保无人机,地面弥雾机适用于芒果冠层中下部及内部病虫害防治,同时该机具较高的雾滴覆盖密度在喷洒杀菌剂时也有明显优势,植保无人机适用于针对芒果上部冠层如蓟马、炭疽等易发于外部花絮的病虫害防治。     

Vitamin C enhanced myocardial differentiation of dedifferentiated fat cells

AIM: In order to observe the myocardial differentiation capacity of the dedifferentiated fat (DFAT) cells treated with vitamin C in vitro. METHODS: DFAT cells were dedifferentiated from the mature rat adipocytes with ceiling adherent culture. The DFAT cells of passage 3 were used in the study. Vitamin C and/or neonatal rat heart tissue lysate were added into the culture medium to induce myocardial differentiation for 3 weeks. The cell morphology was observed under microscope. The myocardial-specific markers, such as cTnT, GATA-4 and NKx2.5, were examined by the methods of immunofluorescence, PCR and Western blot. RESULTS: Mature rat adipocytes dedifferentiated into fibroblast-like DFAT cells after ceiling adherent culture. The DFAT cells spontaneously differentiated into cardiomyocyte-like cells under normal culture condition with a low incidence. After treated with neonatal rat heart cell lysate, the DFAT cells became cardiomyocyte-like cells that had bigger size, longer shape and myotubule-structure. The expression of cTnT, GATA-4 and NKx2.5 was remarkably increased at both mRNA and protein levels as compared with the normal cultured DFAT cells. The expression of cTnT, GATA-4 and NKx2.5 was further increased in DFAT cells after treating with vitamin C. No spontaneous beating cell was observed. CONCLUSION: Vitamin C enhances the differentiation of DFAT cells into cardiomyocyte-like cells.     

Sublytic C5b-9 induces protective autophagy in cultured podocytes

AIM: In podocytes, autophagy occurs at a high basal level and dysregulated autophagy is associa-ted with a variety of podocytopathies. This paper is to investigate the role of autophagy in sublytic C5b-9-induced podocyte injury. METHODS: Sublytic complement C5b-9 stimulation was used as an in vitro model. Autophagosomes were confirmed using monodansylcadaverine (MDC) staining. Immunoblotting was used to measure the change of autophagy-related markers. Cellular morphological changes were observed by Wright-Giemsa staining. Immunofluorescence staining and confocal microscopy were used to detect the expression and distribution of nephrin. The cell viability was assessed by methylthiazol tetrazolium (MTT) assay. The cell apoptosis was assessed by Annexin V-fluorescein isothiocyanate/PI staining. RESULTS: For ensuring sublytic complement injury, the maximal amounts of anti-podocyte antiserum and 160×-diluted normal human serum were used without inducing cell lysis (defined as >5% LDH release). Sublytic C5b-9 promoted autophagy of podocytes in vitro. The proautophagic effect of sublytic C5b-9 manifested in the form of accumulated MDC-labeled vesicles and enhanced the expression of LC3-Ⅱ. Autophagy inhibitor 3-methyladenosine (3-MA) promoted sublytic C5b-9-induced podocyte morphological abnormalities. Compared with the sublytic C5b-9-injured podocytes, 3-MA exposure further decreased the expression of nephrin. 3-MA enhanced sublytic C5b-9-induced podocyte apoptosis. CONCLUSION: Sublytic C5b-9 attack induces autophagy, which may play a protective role against complement-mediated podocyte injury.     

盛荚期喷施亚硒酸钠对大豆籽粒硒含量及单株产量的影响

为了研究喷施亚硒酸钠对大豆籽粒硒含量及单株产量的影响,采用裂区设计,主区为盛荚期喷施不同用量的亚硒酸钠,共5个处理（CK（清水）：0 g/hm²,C1:30 g/hm²,C2: 60 g/hm²,C3: 90 g/hm²,C4: 120 g/hm²）,副区为8个大豆品种。分析8个大豆品种在喷施5种用量亚硒酸钠处理中籽粒硒含量及单株产量变化规律。结果表明,试验地土壤达到富硒水平,在CK处理中,不同大豆品种籽粒硒含量均未达到富硒大豆标准,需要额外喷施硒肥。随着喷施亚硒酸钠用量的增加,8个大豆品种籽粒中硒含量均呈增加趋势,亚硒酸钠用量与大豆籽粒硒含量呈显著或者极显著正相关。8个大豆品种籽粒硒含量均值随亚硒酸钠用量的增加呈上升趋势,与CK相比,C1～C4处理均能极显著提高8个大豆品种籽粒硒含量均值,提高了571.186 %～2 489.831%。亚硒酸钠用量对大豆籽粒硒含量的影响大于大豆品种对大豆籽粒硒含量的影响。喷施亚硒酸钠用量（X）与每种用量下8个大豆籽粒硒含量均值（Y -均）的直线回归方程为Y -均=0.012X+0.003,r =0.986,达到极显著正相关。根据该方程,当大豆籽粒硒含量达到富硒大豆标准（0.1～1 mg/kg）,喷施亚硒酸钠用量的范围为8.08～83.08 g/hm²。分析喷施亚硒酸钠用量与大豆籽粒硒含量的回归方程发现,郑9525的回归方程斜率最大,C4处理中籽粒硒含量达到最大值1.997 mg/kg,籽粒富集硒能力最强;齐黄34斜率最小,C4处理中籽粒硒含量达到最小值1.133 mg/kg,籽粒富集硒能力最弱。在本试验条件下,喷施亚硒酸钠对大豆单株产量没有显著影响。大豆品种对单株产量的影响大于亚硒酸钠用量对单株产量的影响。     

长春市广场树木生长状况与园林植物多样性相关关系的研究

对长春市广场树木生长状况和园林植物多样性之间的关系研究表明:广场树木生长状况的数量化值与用Shannon-Wiener指数表示的园林植物多样性之间有良好的相关关系,相关方程为:G=1.972 2 2.667 86Ht-0.644 7Ht2,相关系数R=0.908 57,估计可靠性P=99.9%。     

3D modeling and reconstruction of plants and trees: A cross-cutting review across computer graphics,vision, and plant phenotyping

This paper reviews the past and current trends of three-dimensional (3D) modeling and reconstruction of plants and trees. These topics have been studied in multiple research fields, including computer vision, graphics, plant phenotyping, and forestry. This paper, therefore, provides a cross-cutting review. Representations of plant shape and structure are first summarized, where every method for plant modeling and reconstruction is based on a shape/structure representation. The methods were then categorized into 1) creating non-existent plants (modeling) and 2) creating models from real-world plants (reconstruction). This paper also discusses the limitations of current methods and possible future directions.     

黄土高原小流域植被特征及其季节变化

植被作为影响水土流失的一个重要因子一直受到广泛关注 ,黄土高原的植被特征随季节发生很大的变化 ,从而对土壤侵蚀发生深刻影响。选择陕北安塞县大南沟小流域 ,于 1998年、1999年和 2 0 0 0年的 5～ 10月 ,每 2周1次对其植被盖度、植被高度和叶面积指数 (L AI)及其季节变化进行了测量和分析。在大南沟流域 ,一半以上的土地是农地 ,种植了玉米、糜子、谷子、马铃署、荞麦和大豆 ,其它土地利用方式有荒草地、休闲地、灌丛、林地和果园。对于农地 ,叶面积指数、作物高度和盖度随季节发生很大的变化 ,农地盖度在春天增长缓慢 ,一般在 8月份才达到其最大值 ;非农地的盖度从 4月份开始增长 ,一般 6～ 7月达到其最大值 ,并保持至 9月甚至 10月份。年降雨总量对植被有很大的影响 ,而单场大降雨对植被特征也有很大的影响     

田间施用硝化抑制剂DMPP对小青菜贮藏过程中硝酸盐与Vc含量的影响

采用田间施药方法,研究了施用硝化抑制剂3,4-二甲基吡唑磷酸盐(3,4-dimethylpyrazolephosphate,DMPP)对冰箱贮藏条件下小青菜可食部分硝酸盐、维生素C(Vc)含量变化的影响。结果表明,在贮藏过程中,施与不施DMPP2种处理小青菜硝酸盐含量呈现先降低后略有升高的趋势,Vc含量呈降低的趋势;DMPP在贮藏前期能够有效地降低硝酸盐含量,延缓Vc含量的降低。贮藏2d后,DMPP处理小青菜硝酸盐、Vc含量分别降低了74.4和9mg·kg-1,而对照处理分别降低了54.4和50mg·kg-1,处理间硝酸盐、Vc降幅差异均达5%显著水平。贮藏2～4d中,DMPP处理硝酸盐、Vc含量分别降低了20.1和91mg·kg-1,而对照处理分别降低了144.7和84mg·kg-1,处理间硝酸盐含量降幅差异达5%显著水平。贮藏4～6d中,对照处理硝酸盐含量升高了60.6mg·kg-1,DMPP处理升高了76.2mg·kg-1,Vc含量分别降低23和117mg·kg-1,且处理间降幅差异达5%显著水平。蔬菜贮藏时间不要过长,以不超过4d为宜。     

污泥土地利用对环境的影响

污泥中含有丰富的有机质及氮、磷等养分，土地利用是污泥处理的重要途径之一。由于污泥的含水率很高，易腐烂发臭，且含有病原菌、重金属及毒性有机物等，在土地利用之前进行稳定化处理是必要的。在污泥的稳定化处理方法中以堆肥化处理较符合我国国情。污泥的土地利用对土壤物理、化学及生物学性质有明显的改良作用，可促进植物生长，提高产量及品质。同时，由于污泥中的重金属及丰富的氮素，使用不当，可导致重金属在土壤与植物中的积累，过量的硝态氮可能污染地表与地下水。