培养条件对酸枣叶片不定梢再生率的影响

以酸枣无菌苗叶片为外植体,选用基本培养基ＮＮ６９和ＷＰＭ,外源激素包括ＴＤＺ、ＩＡＡ、ＩＢＡ和ＮＡＡ,暗培养时间为２、３、４周。采用正交实验设计方法,研究了基本培养基、外源激素及暗培养时间对叶片不定梢再生的影响。结果表明在附加ＴＤＺ１~３ｍｇ/Ｌ的ＷＰＭ和ＮＮ６９上都可诱导不定梢再生,但ＷＰＭ比ＮＮ６９更有效。暗培养时间对不定梢再生也有影响,暗培养３周比２、４周更有利于提高叶片的不定梢再生率。在附加ＴＤＺ１ｍｇ/Ｌ、ＩＡＡ０．１ｍｇ/Ｌ的ＷＰＭ培养基上,暗培养３周后转到光下培养,获得的不定梢再生率达８７．５%。     

Effects of liver regeneration on the proliferation of rat fetal hepatocytes after intrasplenical transplantation

AIM:To study the effect of environment of liver regeneration on the proliferation of rat fetal hepatocytes after intrasplenical transplantation. METHODS:Fetal hepatocytes isolated from 3-week SD rat fetuses bred were transplanted into the spleens of liver regeneration model rats with 70% partial hepatectomy. The cell cycle of the hepatocytes in the remnants liver was analyzed by flow cytometer and the density dimensions of the donor fetal hepatocytes in spleen were measured by image analysis system 7 and 30 days post-transplantation, respectively. RESULTS:Compared with the control group, the proportions of S and G₂ /M cells in the remnants liver were obviously decreased (P＜0.05), but the density dimensions of the donor fetal hepatocytes in spleen increased significantly (P＜0.05) in rats with hepatectomy 7 days post-transplantation. CONCLUSION:The environment of liver regeneration is propitious to the proliferation of fetal hepatocytes after transplantation into spleen.     

香蕉分生小球体途径胚性细胞悬浮系的建立

 以7 个香蕉品种未成熟雄花为外植体均获得了分生小球体(meristematic globules) 。分生小球体的诱导率因基因组型和品种而异, 为15 %～81 % , 分生小球体的特点及开始出现的时间也受基因组型和品种的影响。以分生小球体为起始材料, 建立了‘广东2 号’、‘华农7 号’及‘河口龙牙’3 个品种的胚性细胞悬浮系, 并通过体胚发生途径获得了前两个品种的再生植株。     

苯丙烯酸对黄瓜幼苗光合作用和细胞超微结构的影响

 通过基质栽培研究不同浓度的苯丙烯酸对黄瓜幼苗光合作用和超微结构的影响。结果表明，苯丙烯酸浓度在80 µmol·L 时显著地降低了黄瓜幼苗的叶面积、叶绿素a含量、光合速率、蒸腾速率、根系活力和气孔张开数(P<0.05)；叶绿体和线粒体等超微结构也受到破坏，而浓度在150 µmol·L 时显著减少了全株干质量和气孔总数，细胞器出现解体现象。     

芥蓝游离小孢子培养初报

 以芥蓝的5个基因型为试材，对游离小孢子培养诱导胚状体发生及植株再生进行了初步探讨。结果表明：供体基因型对成功诱导胚状体发生至关重要；振荡培养明显有利于胚状体的发育；改良MS和B培养基对诱导植株再生没有差别。     

铺散诸葛菜的组织培养及植株再生

 以铺散诸葛菜的叶柄及叶片为外植体, 研究了不同的再生方式。在6-BA 和NAA 配合使用的MS 培养基上, 叶柄和叶片外植体有高的成芽率, 具有强的器官再生能力。在2,4-D 与6-BA、NAA 结合的培养基上, 叶柄和叶片有较高的愈伤组织诱导率, 在含6-BA 的培养基上也易于分化。叶柄、叶片再生的芽和经愈伤组织分化而得来的芽快速繁殖后, 在生根培养基上形成完整植株。研究表明铺散诸葛菜具有极强的器官分化能力。     

设施油桃根系的生理生化代谢动态

研究了设施栽培条件下曙光油桃根系的碳氮物质代谢、POD和SOD活性的动态变化。结果表明,在新根的生长季节,根系中可溶性糖、蛋白质含量、游离氨基酸总量、POD、SOD活性的变化与新根的生长具有相同的趋势,淀粉则相反。粗根的淀粉、可溶性糖含量、POD活性高于细根,而蛋白质含量、游离氨基酸总量和SOD活性则变化较大。     

The re-emergence of indigenous forest in an urban environment, Christchurch, New Zealand

Christchurch, the second largest city in New Zealand is a planned city on a coastal plain on the east coast of the South Island. The birth of the city and the subsequent century of development was characterised by colonial values and tree and garden planting with familiar European species along with those from Australia, North America, and eventually all other continents. The image of an “English garden city” with classical parks of oaks and willow-lined rivers became the accepted norm and the way in which the city has been promoted to potential tourists. Gardening is one of the top two recreational activities and exotic species greatly outnumber native species in the flora and in gardens. This has had serious consequences for the highly fragmented and degraded indigenous vegetation and its co-adapted wildlife. A few hardy indigenous species continued to regenerate through this period, but since the 1970s, there has been a progressive change of attitude and interest in reclaiming the natural heritage of the city, manifest in widespread private and public planting of indigenous species and active habitat restoration. In this article we examine the indigenous and exotic shrub and tree components of the Christchurch flora as planted street trees, in domestic gardens, and in parks. We also present data on shrub and tree regeneration in parks and domestic gardens in the city. Indications are that the more sensitive, less intrusive management of urban environments, combined with the greater density of indigenous seed sources, has allowed regeneration of a wide range of indigenous species across a broad spectrum of habitats – from neglected gardens to pavement cracks to exotic plantations. This is despite the competition from the prodigious seed banks and density of exotic trees, shrubs, and ground covers and albeit minimal impacts of introduced browsing and seed eating mammals. If the present trends continue through appropriate management and facilitation, these tentative signs of native forest regeneration should eventually proliferate into a sustainable mixed origin urban forest that resurrects and preserves the natural character of the region.     

不同苜蓿品种对叶片愈伤组织诱导及植株再生的影响

以7个苜蓿品种叶片为外植体,采用3种诱导培养基、5种分化培养基,共15个组合,研究了不同品种间愈伤组织诱导及植株再生的差异。结果表明,不同苜蓿品种间植株再生率差异显著,超级13R在7个苜蓿品种中表现最佳。     

小叶锦鸡儿根瘤固氮活性的动态变化

对多年生小叶锦鸡儿不同生育期根瘤固氮能力、不同树龄小叶锦鸡儿根瘤固氮能力进行调查,结果表明:开花期固氮酶活性最高,此时土壤含氮量和植株含氮量最高;新瘤多发生于果后营养期,植株生物量的峰值出现在结实期;二年生和五年生植株的根瘤固氮能力强于三年、四年生植株,随生长年限的延长,植株生物量逐渐增大,含氮量呈小幅度下降趋势。