Effects of resveratrol on TNF-α-induced injury and expression of MCP-1 in isolated rat pulmonary artery endothelial cells

AIM: To investigate the possible mechanism of resveratrol (Res) on tumor necrosis factor-α (TNF-α)-induced monocyte chemoattractant protein-1 (MCP-1) expression in primary rat pulmonary artery endothelial cells (RPAECs).METHODS: RPAECs were randomly divided into 4 groups:control group, solvent (1% DMSO) group, TNF-α group and Res group. Each group was divided into 1 h, 4 h and 8 h subgroups (n=6 per time point). The TNF-α+C1142 (a rodent chimeric mAb that neutralizes rat MCP-1) group was set up at the 8 h time point. At each time point, the protein and mRNA expression of MCP-1 was measured by Western blot and real-time PCR.RESULTS: Pretreatment of the RPAECs with C1142 significantly down-regulated the expression of MCP-1 (P<0.05). The protein and mRNA expression of MCP-1 was markedly increased in TNF-α group (P<0.05). Notably, incubation with Res down-re-gulated the protein and mRNA expression of MCP-1, which was significantly lower than that in TNF-α group (P<0.05).CONCLUSION: MCP-1 was involved in the process of TNF-α-induced injury of RPAECs. Res down-regulates the expression of MCP-1 in RPAECs, thus attenuating cell injury.     

24-表油菜素内酯对酿酒葡萄白藜芦醇诱导合成的影响

以‘赤霞珠’(Cabernet sauvignon)和‘蛇龙珠’(‘Cabernet gernischt’)葡萄果实为试材,在葡萄浆果生长期设置0(对照)、0.05、0.1、0.5mg·L~(-1)的24-表油菜素内酯(EBR)处理,研究不同质量浓度的24-表油菜素内酯处理对葡萄果皮白藜芦醇(Res)质量分数的影响,同时探讨葡萄果皮中白藜芦醇质量分数与苯丙氨酸解氨酶(PAL)、肉桂酸4-羟基化酶(C4H)、4-香豆酸辅酶A连接酶(4CL)活性的关系。结果表明,在葡萄果实生长发育过程中,‘赤霞珠’和‘蛇龙珠’果皮中白藜芦醇质量分数变化出现2次高峰,分别在花后35d和花后80d。PAL、C4H、4CL活性变化与果皮中白藜芦醇质量分数变化相一致。不同质量浓度的EBR处理对葡萄白藜芦醇有显著的诱导效果,其中以0.1mg·L~(-1) EBR诱导效果最好。由此推断,适宜质量浓度的EBR处理可能激活相关酶的活性,进一步提高葡萄白藜芦醇质量分数。     

紫外分光光度法测定转基因烟草叶片中自藜芦醇的含量

利用紫外分光光度法对转基因烟草叶片中自藜芦醇的含量进行了测定。结果显示,转入花生白藜芦醇合成酶基因的烟草叶片中自藜芦醇浓度约为6．83μg/ml,比野生型材料中约5．11μg/ml的白藜芦醇含量略高。经紫外胁迫诱导后,其叶片中自藜芦醇含量分别提高到10．98μg/ml和9．25μg/ml。白藜芦醇合成酶转人烟草后不仅使目标植物中白藜芦醇含量有所增加,而且使植物自身对紫外胁迫的耐受能力也得到了较大的提高。     

超声提取花生根茎叶中白藜芦醇的工艺优化

[目的]优化花生根茎叶中白藜芦醇超声法提取的工艺条件。[方法]以花生根、茎、叶为原料,采用超声法对白藜芦醇的提取工艺进行研究,通过单因素试验和正交试验确定提取的最佳工艺条件,并通过HPLC法进行分析。[结果]超声提取法提取花生根、茎、叶中白藜芦醇的最佳工艺为：超声频率为15.33 kHz,占空比为1∶2,提取时间为6 min,料液比为1∶25,乙醇浓度为70%,在最佳提取条件下测得白藜芦醇的提取量为885.96μg/g。[结论]该研究得到超声提取法提取花生根、茎、叶中白藜芦醇的最佳提取工艺,为高效提取花生根茎叶中的白藜芦醇提供了新途径。     

葡萄皮白藜芦醇浸提工艺的研究

[目的]优化葡萄皮白藜芦醇的浸提工艺,为合理开发和综合利用葡萄皮奠定基础。[方法]以浸提液中白藜芦醇的提取率为指标,采用单因素试验对葡萄皮白藜芦醇的浸提工艺进行优化。[结果]葡萄皮白藜芦醇的最佳浸提工艺为：浸提温度为50℃,浸提时间为4h,料液比为1：14,浸提方式为水浴静止。[结论]该工艺操作简单,易实现工业化。     

超声波辅助提取花生衣中的白藜芦醇

[目的]以乙醇为溶剂,栗用超声波辅助提取的方法提取花生农中白藜芦醇。[方法]以白藜芦醇的提取率为考察指标,研究花生衣中白藜芦醇提取的优化工艺。[结果]正交试验表明,花生衣中白藜芦醇的适宜提取条件：常温条件下料液比为15：1、乙醇浓度为60％、超声处理时闯为60min、超声功率为150W,白藜芦醇的平均提取率是0．299％。料液比和乙醇浓度是影响白藜芦醇提取率的显著因素。[结论]该工艺简单快捷,可用于花生衣中白藜芦醇的提取。     

白藜芦醇对动脉粥样硬化大鼠核转录因子NF-кB的影响

[目的]研究白藜芦醇（Res）对动脉粥样硬化（AS）大鼠血脂水平和NF-κB的影响,探讨抗AS的部分机制。[方法]制成AS大鼠模型后,设4个组：①模型对照组（高脂饲料）;②低剂量给药组[高脂饲料＋Res30mg/（kg·d）];③中剂量给药组[高脂饲料＋Res60mg/（kg·d）];④高剂量给药组[高脂饲料＋Res120mg/（kg·d）],连续灌胃给药5周后,测定各组血脂和血管壁组织NF-кB阳性细胞表达率。[结果]各给药组TG、TC和LDL-C均较对照组有不同程度的降低,HDL-C较对照组有所升高;免疫组化和蛋白印迹分析均显示各给药组NF-кB阳性细胞表达率明显低于对照组。[结论]白藜芦醇可调节AS大鼠血脂,降低AS大鼠主动脉壁NF-κB的表达,从而抑制AS的形成。     

bukUV-C诱导对花生植株白藜芦醇及其衍生物的影响

白藜芦醇是多种植物中的抗病因子。采用高效液相色谱方法(HPLC),对紫外线辐射后花生植株不同组织部位中白藜芦醇及其衍生物含量进行了检测,系统地比较了花生不同组织部位白藜芦醇及其衍生物的时空含量分布。研究结果表明,紫外线辐射30min,在室温下(25℃)完全避光中放置,可明显提高花生植株中白藜芦醇及其衍生物含量。其中,花生根部Res及其衍生物对UV-C辐射诱导的敏感性远高于茎部和叶片,经紫外处理42h后,花生根部Res含量高达124.47μg/g,是对照的104.55倍。     

花生种子白藜露醇的诱导 与抗黄曲霉侵染关系的研究

通过比较抗—感花生品种受黄曲霉菌侵染后,种子诱导白藜露醇的差异,探讨白藜露醇诱导与花生种子 抗黄曲霉侵染关系。结果表明:受黄曲霉侵染后,花生种子白藜露醇诱导的速度与抗性有关。抗性品种在受侵染 后3d种子内的白藜露醇大量累积并达到峰值,含量提高30倍;而感病品种则在受侵染后4d才达到此峰值。放线 菌素酮和放线菌素D处理后接种试验表明,种子本身含有合成白藜露醇的mRNA,当受外源黄曲霉菌侵染时能激 活与合成白藜露醇相关酶的活性,合成大量白藜露醇。     

Investigation of the Effects of a Dietary Supplement on Insulin and Adipokine Concentrations in Equine Metabolic Syndrome/Insulin Dysregulation

High insulin concentrations are a common clinical feature of equine metabolic syndrome (EMS) and insulin dysregulation. Hyperinsulinemia can induce laminitis, so reduction of insulin concentrations in response to an oral challenge should decrease risk. In human studies, diets containing a polyphenol (resveratrol) led to improvements in insulin sensitivity. In rodents, the addition of leucine to a resveratrol supplement caused a decrease in the amount of resveratrol needed to achieve a clinical effect. We hypothesize a supplementation with a low dose of a synergistic polyphenol and amino acid blend including leucine (SPB+L) would improve metabolic health in EMS/insulin dysregulated horses. Fifteen EMS/ID horses received a high or low dose of SPB+ L daily for 6 weeks. Insulin during an oral sugar test (OST), body condition score, weight, baseline high-molecular-weight (HMW) adiponectin, triglycerides, nonesterified fatty acids, and tumor necrosis factor alpha were assessed before supplementation (PRE) and after supplementation (POST) via paired Student’s t-tests and a repeated-measures mixed-model analysis of variance (significant at P < .05). There were no differences between doses. Horses in the POST group weighed significantly less, had significantly higher baseline HMW adiponectin concentrations, and had significantly lower insulin concentrations at 60- and 75-minute time points (P < .05). Insulin concentrations of the horsesin the POST group, but not in the PRE group, were lower and similar to results from the study conducted three years before the present study (PRIOR) for 0- and 60-minute time points (P < .002). An increased HMW adiponectin level supports increasing insulin sensitivity after supplementation. These results suggest that SPB + L supplementation at either dose leads to improvements in the clinical manifestations of EMS/insulin dysregulation, potentially reducing laminitis risk.