榆黄菇菌丝体多糖提取及抗肿瘤活性研究

液体发酵榆黄菇,从菌丝体中提取粗多糖,研究其体内抗肿瘤活性。用0.2、0.4、0.8 g/kg3个剂量组对荷S180小鼠进行灌胃,结果发现:榆黄菇菌丝体粗多糖得率为2.99%,低、中、高3个剂量组对肿瘤的抑制率分别为30.14%、41.73%和48.87%,同时能提高荷瘤小鼠的胸腺指数和脾指数。榆黄菇菌丝体多糖具有明显的抗肿瘤活性。     

油茶肉质果多糖的分离纯化及单糖组成研究

从油茶肉质果中提取多糖,采用薄层色谱法测定其多糖的单糖组成.结果表明:该多糖的单糖组成为葡萄糖、半乳糖和木糖.     

南瓜8种性状的变异及相关性研究

选用46份具有代表性的南瓜品系,研究不同南瓜品系间多糖含量、可溶性固形物含量、单果质量、单果籽粒数、百粒质量、第1坐果节位、果形指数、产量的变异及不同性状间的相关性。结果表明,南瓜8种性状的变异系数大小依次为果形指数(0.432)、多糖含量(0.414)、产量(0.413)、单果质量(0.335)、单果籽粒数(0.313)、百粒质量(0.240)、可溶性固形物含量(0.196)、第1坐果节位(0.146)。南瓜多糖含量与可溶性固形物含量、果形指数之间相关关系达到极显著和显著水平,相关系数分别为0.5044和0.3405。可溶性固形物与第1坐果节位之间显著相关,相关系数为0.3720。单果质量和产量之间极显著相关,相关系数为0.6677。单果质量、产量和可溶性固形物之间呈现显著的负相关。     

保健品中多糖检测方法的研究进展

通过对现行企业标准中多糖检测方法的总结,分析各种方法的可行性和特点,各种方法有不同的针对性和优点,并通过实际操作归纳出方法中仍需改进的部分,希望在保健品的多糖测定部分能早日出台国家标准或者行业标准。     

香菇多糖Le-Ⅱ-2的分离纯化及其性质研究

采用水提醇沉法从香菇子实体中提取香菇粗多糖,Sevage法脱蛋白后,经DEAE柱层析、Sephdex G-200柱层析后得香菇多糖Le-Ⅱ-2组分.该组分经紫外和红外扫描证明其不含蛋白成分,并具有多糖的性质.     

Effect of active modified atmosphere packaging with different initial gas compositions on nutritional compounds of shiitake mushrooms (Lentinus edodes)

The effect of active modified atmosphere packaging with different initial gas compositions on fresh shiitake mushrooms was investigated. Shiitake mushrooms were packaged under each of the following conditions: (1) high oxygen packaging (HOP), 100% oxygen initially; (2) medium oxygen packaging (MOP), 50% O₂ and balanced with N₂ initially; (3) low oxygen packaging (LOP), 3% O₂/5% CO₂, balanced with N₂ initially. Passive modified atmosphere packaging with air inside initially (AIR) was used as the control treatment. All the packaged samples were stored at 10 °C with 90% RH for 7 days. During storage, gas compositions, electrolyte leakage, crude water-soluble polysaccharides, total phenolic content and free amino acid content were determined. The results showed that ethanol release was high in LOP and AIR but retarded in MOP and HOP. No packaging could prevent the decrease in polysaccharide content decreasing. Both the active and passive modified atmosphere packaging maintained the color and integrity of the mushrooms. The active modified atmosphere packaging had a significant effect on the increase of total phenolic content and total amount of free amino acids. LOP had a harmful impact on mushrooms with high levels of electrolyte leakage. In conclusion, HOP and MOP had a better effect on maintaining the nutritional compounds of shiitake mushrooms.     

高山薇菜中多糖提取条件优化

以粉碎的薇菜粉末为原料,通过水煮法提取中多糖,测得薇菜中多糖的含量达59.133 mg/g。单因素试验、正交优化结果表明,薇菜中多糖提取的最佳条件为温度90℃、提取时间6 h、料液比1∶20。     

杏鲍菇不同菌株生物学特性的研究

比较研究了杏鲍菇4个菌株的菌丝拮抗性、酯酶同工酶酶谱、菌丝生长速度、菌丝体生物量及多糖含量。结果表明，4个菌株问存在遗传差异。菌株X3菌丝生长速度最快，日均长速为0．69cm；菌株X2菌丝体生物量最高，为1．572g／100mL；菌株X1菌丝体多糖含量最低，仅为39.07μg／mg，菌株X2、X4和X3菌丝体多糖含量分别为84．28μg/mg、79．20μg／mg和78．99μg/mg。     

深层发酵灵芝高产菌株筛选初探

将从各地收集到的24个灵芝菌株进行深层发酵培养，通过比菌丝生长速度和发酵生物量，筛选出8个优良菌株。根据菌株间拮抗反应及蛋白酶同工酶分析结果，确定8个菌株中的G2和G15、G33和G27为同一菌株，属同株异名，进一步比较8个菌株的菌丝发酵生物量，胞内，胞外多糖含量等指标，确认菌丝发酵生物量，胞内多糖含量较高的灵芝0770为液体深层发酵首选菌株。     

桑黄多糖的体外抗氧化作用研究

以对DPPH自由基和羟基自由基的清除活性以及还原力为指标,测定桑黄多糖的体外抗氧化作用。结果显示,桑黄多糖具有较强的还原力以及对DPPH自由基和羟基自由基的清除作用,其还原力和自由基清除作用与其浓度呈现量效关系。结果表明,桑黄多糖具有明显的体外抗氧化作用。