丰抗号小麦的选育和若干性状选择的探讨

本文对抗锈、越冬、抗倒伏、熟期和籽粒灌浆等性状的选择进行了探讨.研究结果表明:①正确选配亲本对选育优良品种具有重要的意义.洛夫林10号对锈病和白粉病的抗性突出,且遗传传递力强,在灌浆和落黄等特性的传递方面表现也好.有芒红7号不仅越冬性好,遗传传递力强,而且是一个配合力较强的早熟亲本.它和特晚熟的洛夫林10号杂交后,通过各世代连续“中中选早”选出了中早熟的丰抗7号.可见,二者都是我国北部冬麦区不可多得的好亲本.②两个高杆品种杂交后,选育出了比较矮的后代——丰抗2、5号(株高80—85厘米).此试验表明,在矮化育种中可取材广泛些,除着重应用矮杆基因种质外,还可以选用一些综合性状好的中杆或高杆品种.     

Selection parameters for resistance to Fusarium oxysporum f. sp. cubense race 1 and race 4 on diploid banana (Musa acuminata Colla)

Summary Shoot tip cultures from banana clones susceptible and resistant to Fusarium oxysporum f. sp. cubense (FOC) race 1 and race 4 were grown in vitro in the presence of different concentrations of fusaric acid and fungal crude filtrates or inoculated with a conidial suspension of FOC to assess correlation between in vivo and in vitro behaviour. Explants were susceptible to both filtrate and fusaric acid irrespective to their known field resistance/susceptibility response. No clear linkage between in vivo and in vitro behaviour was observed and our results suggest that the use of crude filtrate or non-host specific toxin (fusaric acid) in a screening programme for selecting a novel resistant genotype of Musa to FOC is not feasible. When peroxidase activity was used as a parameter to discriminate between sesceptibility and tolerance, results were in good agreement with field response of host plant to pathogens. Early enzymatic activity increased in the incompatible host-pathogen interaction but not in the compatible interaction.Abbreviations IBA Indolebutyric acid - 2iP 6-dimethylallylamino-purine - VCG Vegetative Compatibility Group - FOC Fusarium oxysporum f. sp. cubense - IEF isoelectrofocusing     

Analysis of durable resistance to stem rust in barley

Summary Since the mid-1940's, barley cultivars grown in the northern Great Plains of the USA and Canada have been resistant to stem rust caused byPuccinia graminis f. sp.tritici. This durable resistance is largely conferred by a single gene,Rpg1, derived from a single plant selection of the cultivar Wisconsin 37 and an unimproved Swiss cultivar. At the seedling stage, barley genotypes withRpg1 generally exhibit low mesothetic reactions at 16–20° C and slightly higher mesothetic reactions at 24–28° C to many stem rust pathotypes. This resistance is manifested by a low level of rust infection and mostly incompatible type uredia on adult plants.Rpg1 reacts in a pathotype-specific manner since some genotypes ofP. g. f. sp.tritici are virulent on cultivars carrying this gene in the field. Several factors may have contributed to the longevity of stem rust resistance in barley, a) since barley is planted early and matures early, it can sometimes escape damage from stem rust inoculum carried from the south; b) one or more minor genes may augment the level of resistance already provided byRpg1; c) the cultivation of resistant wheat cultivars and eradication of barberry have reduced the effective population size and number of potential new pathotypes ofP. g. f. sp.tritici, respectively; and d) virulent pathotypes ofP. g. f. sp.tritici andP. g. f. sp.secalis have not become established. This situation changed in 1989 when a virulent pathotype (Pgt-QCC) ofP. g. f. sp.tritici became widely distributed over the Great Plains. However,Rpg1 may still confer some degree of resistance to pathotype QCC because stem rust severities have been low to moderate and yield losses light on barley cultivars carrying the gene during the last four seasons (1989–1992). Several sources of incomplete resistance to pathotype QCC have been identified in barley. To facilitate the transfer of resistance genes from these sources into advanced breeding lines, molecular marker assisted selection is being employed.     

Agronomic and Bioenergetic Consequences of Selection for High Groat-Oil Content and High Protein Yield in Oat*

Few studies have examined the effects of selection for high oil or protein content on the chemical composition of cereal seeds. This study was conducted to examine agronomic and bioenergetic consequences that result with changes in the chemical composition of groats of oat (Avena sativa L.). Oat lines with low and high groat-oil content (GO) and with low and high groat-protein content (GP) were chosen from each of five cycles of a recurrent selection program for increasing GO and from each of four cycles of a recurrent selection program for increasing protein yield via elevating GP, respectively. These lines were evaluated at three Iowa locations in 1988. The results indicated that GP was not significantly affected by selection for high GO and that GO significantly increased during selection for high protein yield via elevating GP. Both selection procedures resulted in oat lines with higher groat-energy contents. A bioenergetic cost analysis showed that for GO in the oil population and GP in the protein population to be increased, additional photosynthate needed to be available.     

Study of stomatal parameters for selection of drought resistant varieties in Triticum durum DESF

An analysis was made of the stomatal apparatus of the leaves of five Italian durum wheat varieties, grown under different natural rainfall regimes.Rainfall had a significant influence on both development of the leaves and frequency and size of the stomata. Results were examined with respect to yield stability. Correlations between examined traits were calculated.     

Early Generation Selection for Grain Yield in Narrow-Leaf Lupin (Lupinus angustifolius L.)

Experiments were undertaken to identify an efficient method of selection for yield in early segregating generations of narrow-leaf lupin crosses. A first series of experiments involved simulation of F₂ populations with mixtures of ten cultivars and advanced breeding lines. The performance of genotypes in mixtures sown at different densities was compared with their monoculture [commercial density) yields m the same and the following year. These comparisons provided a basis for assessing the value of different planting designs and different selection criteria in early generation selection for yield. Correlated monoculture yield responses to simulated selection for single plant yield were similar for the honeycomb design and standard selection procedures at both low and commercial densities. These responses were much higher than that for grid selection in a low density population. Correlated monoculture yield responses to selection for other plant characters were substantial only for flowering time and number of pod bearing branches under certain conditions. These responses were greatest for selection in a low density mixture and in the year following selection. A procedure involving selection of the earliest flowering plants and selection within this group of plants with the highest number of pod bearing branches should be an effective approach to early generation yield selection in the narrow-leaf lupin.     

Detection of repeated genotypes among 93 diploid wheat accessions

Summary An objective procedure was developed to find and eliminate repeated genotypes from a set of crop cultivars or accessions which may be used as parents for future plant breeding programs. Nonhierarchical, nonexclusive, exhaustive cluster analysis was used to group 93 diploid wheat accessions using LSD multiple comparisons of accession means based on data measured on seven characters. Beginning with the rarest accessions, i.e. those represented in the fewest clusters resulting from the clusters analysis, a minimum number of the accessions were chosen to represent the phenotypes of all 93 of the accessions. Two analyses were performed, one using data from an irrigated trial, the other from a dryland trial. The dryland trial data was more discriminating in that it required 71 accessions to represent all of the clusters generated from the original 93 accessions while the irrigated trial data required only 60 of the 93 accessions. In addition to the 71 accessions chosen from the dryland trial analysis, the irrigated trial analysis suggested an additional 12 accessions were phenotypically and, therefore, genotypically unique. The total of 83 unique accessions left 10 phenotypically duplicate accessions to be eliminated from the set of potential parents. Four of the 10 duplicated accessions were rare in that they were represented in very few clusters in either the irrigated trial analysis or in the dryland trial analysis. Because their phenotypes were not unique in spite of being rare, we believe such accessions were duplicated genotypically as well as phenotypically. The other six duplicates were common in that they were represented in a large number of clusters. Such accessions may not prove to be duplicates if grown in additional environments (trials), or if measured on additional characters.     

Selection in Faba Bean for Tolerance to Broomrape, Orobanche crenata Forsk.

Six populations of faba bean (Vicia faba L.) were developed through various selection schemes to examine the influence of selection In improving tolerance to Orobanche crenata (Forsk.) in local faba bean stocks. All populations trace their origin to 209 farmers’ seed lots that were collected from Egypt. The entries of each population were grown during the 1982/1983 season in the Orobanche nursery located at Giza in Egypt. Comparison among means of the selected, populations showed that more progress in improving Orobanche tolerance could be achieved from combined selection between and within lots. Between-lots selection was less efficient than single-plant selection. Results, of this study show that considerable progress could be achieved from selection for Orobanche tolerance in local faba bean germplasm. There was sufficient residual genetic variation in the selected populations to expect substantial progress from further selection for most tolerance traits.     

An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: The basic concepts

Recognizing the enormous potential of DNA markers in plant breeding, many agricultural research centers and plant breeding institutes have adopted the capacity for marker development and marker-assisted selection (MAS). However, due to rapid developments in marker technology, statistical methodology for identifying quantitative trait loci (QTLs) and the jargon used by molecular biologists, the utility of DNA markers in plant breeding may not be clearly understood by non-molecular biologists. This review provides an introduction to DNA markers and the concept of polymorphism, linkage analysis and map construction, the principles of QTL analysis and how markers may be applied in breeding programs using MAS. This review has been specifically written for readers who have only a basic knowledge of molecular biology and/or plant genetics. Its format is therefore ideal for conventional plant breeders, physiologists, pathologists, other plant scientists and students.     

Powdery mildew resistance in barley landrace material. I. Screening for resistance

A total of 4,681 accessions of Hordeum vulgare landrace material from Ethiopia, East Mediterranean, Near East, Nepal and China were sown in the field and subjected to the natural powdery mildew epidemic in Denmark. Apparently resistant accessions were selected. Selfed progeny from them were retested and reselected in subsequent years at four locations in Denmark. Finally, 16 promising donors of resistance were retained. They were characterized in the field and tested in the seedling stage for reaction to up to 72 different isolates of the powdery mildew fungus. The absence of the corresponding virulences in the Danish airborne powdery mildew population was ascertained in five years. The resistances in the 16 donors are apparently mutually different and from known sources of powdery mildew resistance in barley. This revised version was published online in July 2006 with corrections to the Cover Date.