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Kate L. Hepworth-Warren David M. Wong Nyomi L. Galow-Kersh Jackie M. Williams 《Journal of Equine Veterinary Science》2014
A placental mass was observed in an otherwise-healthy Quarter Horse mare; subsequently, the mare's 52-day-old foal was examined because of hind limb ataxia, urinary incontinence, and raised lesions of the distal limbs. Clinical and biochemical findings were supportive of liver disease and lumbosacral injury. Ultrasonographic evaluation of the abdomen suggested a liver mass, which was confirmed with computed tomography (CT) and determined to be neoplastic via histopathologic evaluation of a liver biopsy sample. Initial histopathology suggested a germ cell tumor. Regions of lysis affecting both femurs and third metacarpal bones and a complete oblique sagittal fracture through the body of the first sacral vertebra were present on CT. Supportive care was provided until CT confirmation of diffuse hepatic neoplasia and vertebral fracture. Necropsy revealed a large multinodular mass within the liver and a pathologic fracture of the first sacral vertebral body. Histopathologic comparison between neoplastic cells examined from the placenta and foal confirmed metastases of a germ cell tumor. Placental tumors are rare in all species; however, when noted in horses, clinicians should be aware of the possibility of metastatic spread to the foal. 相似文献
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Behrouz GHARESI-FARD Jaleh ZOLGHADRI Eskandar KAMALI-SARVESTANI 《The Journal of reproduction and development》2014,60(4):261-267
The placenta is a unique pregnancy-related tissue and plays a key role in occurrence of unexplained recurrent pregnancy loss
(URPL). Abnormal placentation might play a key role in occurrence of URPL. Therefore, the purpose of this study was to compare the
human placental proteome between URPL placentas and normal placental matched for gestational week. Total placental proteins were
extracted, and the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) technique was used for separation of the placental
proteomes. Protein spots differentially expressed between URPL and normal placentas were selected and identified by the
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF/TOF) technique after being digested in the
gel. Moreover, quantitative real-time PCR and Western blot techniques were used to confirm the differential expression mass
results for some differentially expressed proteins. The results indicated that at least 19 protein spots were differentially
expressed between URPL and normal placentas (P < 0.05), and twelve of them were successfully identified. While only two
proteins were downregulated (calumenin and enolase 1), the remaining ten spots (actin gamma 1 propeptide, cathepsin D
prepropeptide, heat shock protein gp96, tubulin beta, tubulin alpha 1, glutathione S-transferase, vitamin D binding protein,
prohibitin, actin beta, apolipoprotein A-I) showed increased expression in URPL cases in comparison with normal placentas.
Real-time PCR also confirmed the downregulation of calumenin and upregulation of prohibitin and apolipoprotein A-I at the mRNA
levels. In conclusion, the results of the present study showed that alteration in the expression of proteins involved in
proliferation and migration of endothelial cells as well as control of coagulation by these cells might play an important role in
the pathogenesis of URPL. 相似文献
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以辣椒绿熟果(果实绿熟时期采摘)和红熟果(果实红熟时期采摘)为试材,研究其保存在不同温度条件下辣椒素和二氢辣椒素含量的变化规律。结果表明:不同的温度保存下,胎座或果肉的辣椒素含量变化趋势均与二氢辣椒素一致。短时期低温(0℃)保存有利于保持采后辣椒果实辣椒素(二氢辣椒素)高含量,长时间保存时,常温(4℃~25℃)保存有利于保持辣椒素(二氢辣椒素)高含量。同一保存温度下,果肉辣椒素(二氢辣椒素)含量变化趋势与胎座相反。同一温度处理下,不同熟期果实辣椒素(二氢辣椒素)含量变化规律基本一致。 相似文献
35.
Manso Filho HC Costa HE Wu G McKeever KH Watford M 《Veterinary research communications》2009,33(2):175-182
In most mammalian species the developing fetus utilizes large amounts of glutamine derived both from the maternal circulation
and synthesized de novo in the placenta. The present study was designed to determine the role of the placenta in glutamine
synthesis in the horse. The placentae from eight Standardbred mares were sampled immediately after parturition together with
additional tissues obtained at necropsy from three Standbred mares during diestrous. Glutamine synthetase protein was detectable
in the non-pregnant horn of the placenta in amounts similar to those seen in gluteus muscle, but the amount in the pregnant
horn was two times greater than in the non-pregnant horn. Glutamine was the second most abundant amino acid in amniotic fluid
at a concentration of 310 ± 26 μmole/L with that of glycine being 535 ± 48 μmole/L. The most abundant amino acids in placental
tissue were glycine (3,732 ± 194 μmole/Kg), glutamate (3,500 ± 343 μmole/Kg) and glutamine (2,836 ± 208 μmole/Kg). The results
illustrate the importance of glutamine to the equine fetus and establish that the placenta, particularly the pregnant horn,
has considerable capacity for glutamine synthesis. 相似文献
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[目的]通过比较正常孕妇与妊高症患者胎盘中Ghrelin及其受体GHS-RmRNA水平的变化,探讨Ghrelin及其受体与妊高症的发病是否相关。[方法]采用实时荧光定量RT-PCR技术检测了正常孕妇胎盘和妊高症患者胎盘中Ghrelin及其受体GHS-RmRNA水平相对表达量的变化。[结果]正常孕妇胎盘组织中mRNA水平与妊高症患者胎盘组织中mRNA水平无显著性差异,但Ghrelin受体GHS-R在两者之间的mRNA水平有显著性差异(P〈0.05)。[结论]Ghrelin及其受体在妊高症患者和正常孕妇胎盘中表达的相对变化表明,Ghrelin受体缺乏可能成为妊高症发病的因素之一。 相似文献
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Chiyo KITAYAMA Motoki SASAKI Hajime ISHIKAWA Toshihiro MOGOE Seiji OHSUMI Yutaka FUKUI Teguh BUDIPITOJO Daisuke KONDOH Nobuo KITAMURA 《The Journal of reproduction and development》2015,61(5):415-421
The structure and functions of placentas were examined in 3 species of rorqual whales, common minke (Balaenoptera acutorostrata), Bryde’s (B. brydei) and sei (B. borealis) whales, with the aim of confirming the structural characteristics of the chorion, including the presence of the areolar part, and clarifying steroidogenic activities and fetomaternal interactions in the placentas of these whales. Placentas were collected from the second phase of the Japanese Whale Research Program under Special Permit in the North Pacific (JARPN II). Histological and ultrastructural examinations revealed that these whale placentas were epitheliochorial placentas with the interdigitation of chorionic villi lined by monolayer uninucleate cells (trophoblast cells) and endometrial crypts as well as folded placentation by fold-like chorionic villi. Moreover, well-developed pouch-like areolae were observed in the placentas, and active
absorption was suggested in the chorionic epithelial cells of the areolar part (areolar trophoblast cells). Berlin blue staining showed the presence of ferric ions (Fe3+) in the uterine glandular epithelial cells and within the stroma of chorionic villi in the areolar part. An immunohistochemical examination revealed tartrate-resistant acid phosphatase (TRAP; known as uteroferrin in uteri) in the cytoplasm of glandular cells and areolar trophoblast cells. This result suggested that, in cetaceans, uteroferrin is used to supply iron to the fetus. Furthermore, immunoreactivity for P450scc and P450arom was detected in trophoblast cells, but not in areolar trophoblast cells, suggesting that trophoblast cells synthesize estrogen in whale placentas. Therefore, we herein immunohistochemically revealed the localization of aromatase and uteroferrin in cetacean placentas during pregnancy for the first time. 相似文献
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