3.3%1-甲基环丙烯微囊粒剂（聪明鲜~（TM））熏蒸对常温贮藏柿子腐烂率和硬度的影响

以满城磨盘柿为试材,研究了3.3%1-甲基环丙烯微囊粒剂（聪明鲜TM）熏蒸对常温贮藏柿果腐烂率和硬度的影响。结果表明：采用3.3%1-甲基环丙烯微囊粒剂熏蒸,可以明显延缓柿子果实硬度下降,降低柿子腐烂率,延长保存时间。     

柿资源的研究现状

从提取、分离纯化及成分分析、药理及应用等方面对近30年来柿资源的研究进行了综述,并展望其进一步研究和开发前景。     

1-MCP和CO_2处理对柿贮藏期和货架期品质的影响

研究了不同浓度的1-甲基环丙烯(1-MCP)和95%二氧化碳(CO2)结合处理对金瓶柿低温贮藏期(0℃下0~60 d)及其后的货架期(20℃下放置5 d)品质的影响。结果表明:经0.5μL/L 1-MCP+95%CO2处理的柿果在低温贮藏期间乙烯生成速率高峰出现时间迟于未经1-MCP处理(CK)的;经此处理的柿果在低温贮藏期及其后的货架期间,其色度值低于CK的,硬度显著高于CK的,而褐变指数和可溶性单宁含量与CK差异不显著。因此,用0.5μL/L 1-MCP+95%CO2对采后的金瓶柿果进行处理,可以获得较好的效果。     

淳安县柿树资源调查

１９８６－１９９０年唐村柿子品种调查的基础上，于１９９６年在全 范围内又进行了１次调查，经整理共有１２个品种类型，在群众历史选择下形成了以鲜食品种千岛无核柿和加工品种冬柿类型为主栽品种的农家地方品群群，并进行基地造林，选优良单株，建立采穗圃等工作。     

山西省柿子优良品种

山西省共有柿子品种88个,优良品种27个,本文研究了其中10个主要优良品种的形态特征,经济性状、生长特性以及发展前景等,为山西西省发展柿子生产提供了科学依据。     

Adventitious Shoot Regeneration from Leaf of Uenishiwase Persimmon

Effects of basal mediums, hormones and their concentrations on the shoot regeneration from leaf of sweet persimmon (Diospyros kaki Thunb. Cv. Uenishiwase) were studied by orthogonal design trial. The result showed that modified Murashige and Skoog [MS (1/2 N)] was the most optimum for the regeneration and 1/2 MS was better than MS. Shoot percentage in the medium containing 4.0 mg L-1 ZT(zeatin) was much higher than that of other two concentrations, among which 2.0 mg L-1 ZT was much better than 1.0 mg L-1 ZT and shoot percentage in the concentration of 1.0 mg L-1 ZT was only 4%. There were no any beneficial effects when supplementing IAA in the medium. Shoot percentage and average shoots perexplantsweredramaticallydecreasedinthe 2.0 mg L-1 IAA. Data in the orthogonal trial indicated that ZT was the most effective factor in the shoot regenerating of Uenishwase persimmon and basal medium was important too, but IAAhad no any beneficial effects at all. In the orthogonal trial, the best result was achieved in MS (1/ 2 N) medium containing 4.0 mg L-1 ZT and 1.0 mg L-1 IAA, in which shoot percentage and average shoots per explants were 86% and 2.2, respectively.     

Phenolics concentration and antioxidant capacity of different fruit tissues of astringent versus non-astringent persimmons

Astringent and non-astringent persimmon fruits were compared for phenolic compounds, and their antioxidant capacity was studied in both peel and pulp. Fruits that were astringent generally had significantly higher concentrations of phenolics and greater capacities in either tissue. Pulp from astringent persimmons had more phenolics and higher capacity whereas the same was true for peels from non-astringent fruits. Among the methods tested for determining antioxidant capacity (ABTS, DPPH, FRAP, TBARS, SRSA, and HRSA), ABTS showed the best correlation with phenolics concentration while HRSA assays were not sufficiently sensitive to distinguish among genotypes or between fruit tissues. Therefore, we suggest that, for monitoring antioxidant capacity in persimmon, the ABTS method is most suitable.     

柿树组织培养中玻璃化现象的发生与防治

植物组织培养中玻璃化现象的发生极为普遍,是影响植物离体快繁的重大障碍。本试验以柿树为材料,对正常苗与玻璃苗的形态学差异进行了观察,研究了玻璃化现象发生的原因及防治方法。结果表明:提高培养基中琼脂粉的含量(达10g/L),可降低培养基的水势,尤其是降低了试管苗的水势,能有效地防止玻璃化现象的发生,同时有利于继代苗的增殖和生长;适当减少每瓶中接种数目及缩短继代培养时间,也可降低玻璃苗的发生频率。     

常温下柿鲜Ⅰ对木洼柿和火柿的保鲜

刚转黄的木洼柿和火柿用柿鲜1保鲜剂处理后装入塑料袋中,置室内常温下可保鲜3个月以上,基本上保持鲜柿的风味和品质,并保持较高的硬度。柿子的呼吸类型较特殊,介于跃变型与非跃变型之间。多聚半乳糖醛酸酶(PG)和纤维素酶对柿子的软化起很大的作用,其活性增加的原因之一是新合成了蛋白质。柿鲜1保鲜机理主要是抑制果实内部乙烯产生和推迟呼吸峰,抑制PG和纤维素酶等水解酶的活性,从而减缓不溶性果胶和纤维素等的水解、以延迟果实衰老。     

柿和君迁子试管苗茎尖玻璃化法超低温保存及再生植株遗传稳定性研究

对柿(Diospyros kaki Thunb.)和君迁子(D. lotus L.)试管苗茎尖玻璃化法超低温保存及再生植株遗传稳定性进行了研究。结果表明,试管苗在添加有蔗糖0.3 molL-1的改良MS培养基 (KNO3和NH4NO3减半)上,于低温6±1℃下锻炼6周后,切取侧芽茎尖1.5～2.0 mm在含蔗糖0.7 molL-1的改良MS培养基上预培养2 d,室温(20℃)下装载液(2.0 molL-1甘油 0.4 molL-1蔗糖)过渡20 min,0℃下玻璃化液PVS2(30%甘油 15%乙二醇 15%二甲基亚砜 0.4 molL-1蔗糖)处理80 min,换成新鲜的PVS2后投入液氮保存1 d,40℃水浴化冻1 min,含蔗糖1.2 molL-1的改良MS培养基洗涤20 min,接种于含0.2 mgL-1 CPPU、1.0 mgL-1 BA、0.05 mgL-1 IAA、500 mgL-1 可溶性PVP、30 gL-1蔗糖和7 gL-1琼脂的改良MS培养基(再生培养基)的滤纸上,暗处理1 d后转移到新鲜的上述再生培养基中,暗培养1周后转到正常光下,再生率超过30%;再生植株通过细胞学和AFLP标记检测,没有发现核DNA含量、染色体数目和AFLP谱带的改变。该结果为柿属植物种质资源的长期保存提供了理论依据。