Mechanism of AKT1 negatively regulating breast cancer cell migration

AIM: To investigate the molecular mechanism and downstream signaling pathway by which AKT1 inhibition regulates breast cancer cell migration. METHODS: RNA interference was used to knockdown the expression of AKT1. Western blot assay was performed to examine the expression of AKT1 total protein, β-catenin total protein and β-catenin nuclear protein. Immunofluorescence was used to examine the cellular localization of β-catenin. Transwell assay was used to investigate whether β-catenin nuclear accumulation as an alternative pathway was responsible for breast cancer metastasis induced by AKT1 inhibition. RESULTS: The total protein expression of AKT1 was decreased in MCF-7 and MDA-MB-231 cells treated with AKT1 siRNA. A significant increase in the protein expression of β-catenin was observed in MCF-7 cells and MDA-MB-231 cells treated with AKT1 siRNA. Immunofluorescence staining showed that MCF-7 cells and MDA-MB-231 cells displayed strong β-catenin staining in the cytoplasm and nucleus after knockdown of AKT1 expression. The ability of tumor cell migration increased dramatically after treated with AKT1 specific siRNA in the breast cancer MCF-7 cells and MDA-MB-231 cells in Transwell assay. XAV-939 reversed breast cancer cell migration induced by knockdown of AKT1 expression. CONCLUSION: β-catenin nuclear accumulation contributes to AKT1 inhibition-mediated breast cancer cell migration.     

小麦旗叶衰老过程中Rubisco周转初探

为了探讨叶片衰老过程中Rubisco的合成与降解的机理,在抽穗期对小麦进行了增施¹⁵N同位素标记的(NH₄)₂SO₄处理以延缓旗叶的衰老进程,同时对旗叶中Rubisco及Rubisco的组成成分LSU、SSU的含量及其相应的¹⁵N丰度进行了测定。结果表明,Rubisco及LSU、SSU等的含量均增加;且在小麦旗叶光合功能高值持续期,Rubisco及LSU、SSU等的丰度均处于较高水平,表明Rubisco仍处于动态的降解、合成这一周转过程中,仍有Rubisco的重新合成,但Rubisco同时也被不断地降解;而在光合功能低值期,Rubisco及LSU、SSU等的¹⁵N丰度均处于低水平,且基本无变化,表明此时无Rubisco的重新合成。另外土壤¹⁵N丰度的降低说明植株在生长发育后期仍有较强的从土壤中吸收氮素的能力。因此抽穗期施氮肥对小麦产量的提高有重要意义。     

氮磷的不同供应比例和频度对藻类水华形成的影响

为了探讨氮磷的不同供应比例和频度对藻类水华形成的影响,本研究设计了7种氮磷添加比例（质量比）：只添加氮（以＋N表示）、50：1、20：1、7：1,1：1、1：7和只添加磷（以＋P表示）;2种添加频度：频度较大的L系列（实验前期每日均进行添加）,频度较小的S系列（只在实验过程中添加1次）。两种添加频度下,相同添加比例的处理的营养盐添加量相近。在处理＋N、50：1、20：1、7：1和1：1中以N浓度为基准进行相应比例的P添加,在处理1：1、1：7和＋P中以P浓度为基准进行相应比例的N添加。在1：1处理缸中N、P净增加均约为2.4mg·L-1。实验水体来自一个浮游植物丰富但没有微囊藻水华的天然富营养池塘。实验于2007年8月1日—8月13日在室外采用玻璃缸进行。结果表明,两种添加频度以及不同氮磷添加比例下,实验过程中出现的水华种类均为微囊藻（Microcysti sspp.）水华,没有固氮藻类水华出现。在两种不同添加频度下,微囊藻水华在处理＋N、50：1、20：1、7：1和1：1中明显形成,而处理＋P和1：7中,微囊藻水华的出现会晚几天或者水华现象不明显,这表明本实验中单独添加氮比单独添加磷对微囊藻水华形成的促进作用要明显些。水体中微囊藻水华的出现与适宜的氮磷比例添加有关,氮磷添加比例适宜时,两种不同的添加频度下均可出现微囊藻水华,但氮磷营养盐的不同添加比例和频度均没有导致固氮藻类水华的出现。     

宣威市紫色土辣椒氮磷钾肥配比试验研究

对紫色土辣椒氮、磷、钾适宜施用量研究表明，紫色土氮磷钾肥对辣椒产量的影响顺序是磷肥〉氮肥〉钾肥。磷是限制辣椒产量的主导因子。在施用菜籽饼肥600kg／hm^2，折N31．2kg／hm^2、P2O5 11.7kg／hm^2、K2O 8．1kg／hm^2的基础上，辣椒产量≥20400kg／hm^2的氮磷钾优化组合施肥范围：N=113．9～204．6kg／hm^2，P2O5=145．9～177．2kg／hm^2，K2O=49．3～153．8kg／hm^2，N：P2O5：K2O=1：0．87～1．28：0．43～0．75。建议氮、磷、钾肥适宜用量为：N159．2kg／hm^2，P2O5 161．5kg／hm^2，K2O 101．5kg／hm^2，N：P2O5：K2O=1：1．01：0．64，辣椒最高产量预测值为21961．5kg／hm^2，施肥利润5417．7元／hm^2，投产比1：5．00。适量的氮、磷、钾营养使得养分比例平衡，有利于增加辣椒收果数，提高鲜椒产量，从而增加效益。     

一个普通小麦-大赖草易位系T01的选育与鉴定

将抗赤霉病的普通小麦-大赖草Lr.2染色体单体异附加系花粉经过60Co-γ射线处理, 然后给感赤霉病的普通小麦品种“扬麦5号”授粉, 杂交后代连续2年进行赤霉病抗性单株接种鉴定, 从中选育出一个普通小麦-大赖草异易位系。 经过染色体荧光原位杂交和Giemsa C-分带鉴定, 易位发生在普通小麦4B染色体长臂和大赖草第2条染色体(L     

松黄叶蜂的种群动态研究

研究了松黄叶蜂卵、幼虫在油松针叶上的数量变动规律和幼虫的种群动态 ;根据松黄叶蜂各虫期、虫态林间存活率和死亡率调查 ,推算出松黄叶蜂自然种群消减动态 ,提出了松黄叶蜂的防治对策     

Ontogenic Changes in Growth and Assimilate Distribution as Influenced by N Supply in Rapeseed-mustard

Brassica juncea , cv. Pusa Bold, and Brassica campestris , cv. Pusa Kalyani, were raised under field conditions with varying levels of N fertilization, i.e. 0, 40, 80 and 120 kg N ha⁻¹. Changes in dry matter accumulation in various plant parts as influenced by N supply were measured throughout the crop ontogeny. N supply up to 120 kg had an increasing effect on the growth of leaves, stem and pods during the entire period till maturity. Analysis on the assimilate distribution pattern revealed that 84–87% of the total was accumulated during the post-flowering phase. Dry matter partitioning profile during the ontogeny indicated that about 72–83 % of the total DM was accumulated in the leaves, while 17–28 % in the stem, in the preflowering phase. This trend was found nearly opposite during the postflowering phase. At maturity, however, stem and pods accounted for nearly equal amounts of dry matter accumulation. Among the genotypes, Pusa Bold recorded significantly higher DM accumulation and seed yield over Pusa Kalyani which, on the other hand, exhibited a better assimilate-partioning ability than the former. Nitrogen levels had a favourable effect on LAI, LAD and CGR in both genotypes. Highly significant correlations were obtained between seed yield vs. LAI, LAD and CGR. N fertilization up to 120 kg ha⁻¹ was found beneficial in enhancing growth and yield of rapeseed-mustard.     

Morphogenetic Effects of Different Mineral N-compounds on Roots of Maize (Zea mays L.), Spring Barley (Hordeum vulgare L.) and Broad Bean (Vicia faba L.)

Plants of maize ( Zea mays L.), spring barley ( Hordeum vulgare L.) and broad bean ( Vicia faba L.) were raised in tubes filled with a soil-sand mixture and supplied with nutrient solution. The nutrient supply was varied with regard to concentration and typ of compound of "N". Due to the relatively high concentrations of "N", dry matter of shoots and roots was reduced in comparison to the control; root morphology was affected in terms of root length and root thickness. The N-compounds, used in the experiment, exerted quantitatively different influences on dry matter production and root morphology, NH₄NO₃ being the N-form which had the weakest detrimental effects. There was no specific response to NH⁺₄ or NO⁻₃ but a strong influence could be shown for NH₄Cl and N_aNO₃. Probably not only the concentration of "N" and the form of "N" have a specific influence, but also the cations and anions associated with the N-containing ion are effective.