锗硒对高原牦牛抗氧化功能系统的影响

选用青藏高原东部的黄河首曲草原年龄、胎次、营养状况相近的产奶牦牛40头,随机分4组,在同等条件下进行试验,探讨β–羧乙基锗倍半氧化物(Ge-132)、硒(Se)对抗氧化系统功能的影响以及锗(Ge-132)、硒(Se)、铜(Cu)、锌(Zn)在抗氧化过程中的相互关系,和全血Ge-132、Se、Cu、Zn含量的变化。试验期共50 d, 每隔10 d采集血样, 测定血清谷胱甘肽过氧化物酶(GSH-PX),铜锌超氧化物歧化酶(CuZn-SOD)活力及脂质过氧化物丙二醛(MDA)含量和全血中的Ge-132、Se、Cu、Zn含量,并做相关性分析。结果表明:给产奶牦牛适当补充Ge-132、Se能提高机体抗氧化系统的功能,提高全血Ge-132、Se及Cu的含量,降低全血Zn的含量,并且Ge-132、Se联合应用效果优于Ge-132、Se单独应用,Ge-132、Se联合应用具有协同抗氧化作用。     

DGPS数据分析及定位精度研究

本文进行了DGPS数据分析及定位精度研究。通过试验分析表明，Ag132GPS接收机接收到的卫星信号数可以满足使用卫星数量的要求。采用伪距差分纠偏后，Ag132GPS接收机的DGPS单点定位精度可以达到0．232m，满足亚米级的定位精度。     

养殖用水中孔雀石绿和结晶紫及其代谢物的测定

建立了用高效液相色谱紫外、荧光检测器同时检测养殖用水中的孔雀石绿、结晶紫及其代谢物隐色孔雀石绿和隐色结晶紫的分析方法。孔雀石绿和结晶紫用紫外检测器588 nm波长检测;隐色孔雀石绿和隐色结晶紫用荧光检测器检测,激发波长:265 nm,发射波长:360 nm。孔雀石绿和结晶紫的线性范围5.0~2500μg/L;隐色孔雀石绿和隐色结晶紫的线性范围1.0~1 000μg/L,四种物质的检出限均为0.5μg/L,回收率均在70%以上,RSD15%,此方法也可用于地下饮用水中孔雀石绿、结晶紫、隐色孔雀石绿和隐色结晶紫的检测。     

白鲢骨骼肌丝氨酸蛋白酶抑制剂对Jarkat,Sp20,MG63细胞增殖的影响

应用MTT法分析了白鲢骨骼肌丝氨酸蛋白酶抑制剂对急性T细胞白血病细胞Jarkat、人骨髓瘤细胞Sp20和人骨瘤细胞MG63生长的影响。结果表明:白鲢骨骼肌丝氨酸蛋白酶抑制剂对以上3种肿瘤细胞的增殖都有抑制作用,而且抑制作用呈剂量依赖性;该丝氨酸蛋白酶抑制剂在0.1 g/L浓度下对Jarkat细胞的增殖有明显的抑制作用。     

东北春大豆熟期组的划分与地理分布

东北春大豆区是我国大豆主产区,大豆品种生育期特性是最主要的生态特性。美国建立的大豆熟期组制度已为全世界采纳。熟期组归类表述了品种最主要的地理生态特性,有利于全世界不同地区间的引种、交流和育种方案设计。本研究搜集了我国东北地区数十年来育种或生产上广泛使用的地方和育成的共计361份品种,于2012-2014年在东北大豆不同气候生态区的9个试验点,以美国和国内已明确MG000~MGⅢ熟期组的品种为标准,进行东北地区品种熟期划分的研究。鉴定方法为先根据标准品种相邻熟期组生育日数平均值的1/2为界,划定不同熟期组在该环境的范围,初步划定各品种的熟期组归属,然后统计各品种在不同环境的熟期组归属次数,结合考虑该品种适应的生态条件,最终确定其熟期组归属。本研究认为东北地区早春土壤墒情较好,播种后种子开始吸水萌动,而完全成熟则在完熟期(R8时期),大豆的全生育期应为从播种到R8时期的生育日数。获得结果如下:(1)确定了不同试验点/生态亚区各熟期组划分的生育期天数范围和各熟期组鉴定的最佳试验点/生态亚区,具体的说以北安和扎兰屯作为MG000和MG00熟期租适宜的鉴定地点,克山和牡丹江作为MG0和MGⅠ适宜的鉴定地点,铁岭作为MGⅡ和MGⅢ适宜的鉴定地点;(2)361份东北春大豆归入MG000~MGⅢ共6个熟期组;(3)揭示了不同熟期组在东北地区的地域分布,大致上MG000和MG00主要分布在黑龙江北部及内蒙古北部,MG 0和MG I主要分布在黑龙江中南部,MGⅡ主要分布在吉林省,MGⅢ主要分布在辽宁省;(4)提出了一批东北地区各熟期组鉴定的本地区标准品种;(5)提出我国东北地区熟期组鉴定的方法,即先在当地将待鉴定的品种生育期天数与标准品种的表现或者本文所给出的各熟期组在当地的表现进行初步划分,然后按照其熟期组划分结果安排在适宜的鉴定点进行统一鉴定,经比对后确定其熟期组的归属。     

Effect of MG132 on Aβ generation in SH-SY5Y cells

AIM: To observe the influences of different concentrations of MG132 on apoptosis and beta-amyloid protein(Aβ) generation in SH-SY5Y cells, and to explore the underlying mechanism.METHODS: SHSY-5Y cells were incubated with MG132 for 24 h. The final concentrations of MG132 were 2.5, 5 and 10 μmol/L. The cell viability was determined by MTT assay. The cell apoptosis was assessed by flow cytometry. The levels of Aβ were measured by ELISA. The relative protein levels were detected by Western blot.RESULTS: In the SH-SY5Y cells, MG132 reduced the cell viability, induced the cell apoptosis, increased the level of Aβ, and increased the expression of the related proteins for Aβ generation in a concentration-dependent manner.CONCLUSION: MG132 induces apoptosis and increases the levels of Aβ_1-42 and Aβ_1-40 by regulating the proteins related to Aβ generation in the SH-SY5Y cells.     

Effects of proteasome inhibitor MG132 on proliferation of neural stem cells in subventricular zone

AIM: To investigate the role of proteasome in the degeneration of neuron in age-related diseases such as Parkinson disease (PD) by observing the effects of proteasome inhibitor MG132 on the proliferation of neural stem cells (NSCs) in the subventricular zone (SVZ).METHODS: Stereotaxic microinjection of proteasome inhibitor MG132 at a dose of 10 μg into the left lateral ventricle of 90-day-old mice was performed.The control mice were received the same volume of DMSO into the left lateral ventricle.Three days, 7 days and 14 days after injection, the total proteins isolat from SVZ were subject to proteasome activity assay using the fluorescence microplate reader.Meanwhile, the mice were administered with 5-bromo-2'-deoxyuridine (BrdU) by intraperitoneal injection.The numbers of BrdU-positive cells were counted to examine the effect of MG132 on the proliferation of NSCs.RESULTS: After microinjection of MG132 into the left lateral ventricle, the proteasome activity in SVZ was significantly decreased on day 3 and day 7 post-injection (P<0.05) as compared with the control animals.Furthermore, the numbers of BrdU⁺ cells in SVZ were significantly reduced to 21±4 on day 3 and to 22±3 on day 7 post-injection (P<0.05).After long periods of treatment with MG132, the proteasome activity in SVZ was restored to normal level on day 14 post-injection.At the same time, no statistical difference of BrdU⁺ cells between the mice treated with MG132 (82±4) and DMSO (67±6) was observed (P>0.05).CONCLUSION: Short-term injection of reversible proteasome inhibitor MG132 attenuates the proteasome activity in SVZ, which in turn inhibits the proliferation of NSCs, suggesting that the proteasome activity may be closely related to the proliferation potential of NSCs.     

锗-132对高氟低硒地区奶牛自由基代谢的影响

为了探讨锗 －１３２对奶牛抗氧化系统功能的影响以及锗－１３２与元素硒、锌、铜在抗氧过程中的相互关系，选用高氟低硒环境下饲养的奶牛２５头，在基础日粮相同的条件下进行３个月的。将实验牛随机分组，每组５头，共５组，Ⅰ组为对照组；Ⅱ组加锗 －１３２；Ⅲ组加锗－１３２，硒：Ⅳ组加锗－１３２、硒、锌；Ⅴ组加锗－１３２、硒、锌、铜。定期采集血样，测定清中谷胱甘肽过氧化物酸疼（ＧＳＨ－ＰＸ）、超氧化物歧化酶的活性     

Considerations on the Use of Malachite Green in Aquaculture and Analytical Aspects of Determining the Residues in Fish: A Review

Aquaculture in Brazil has shown expressive development since the 1990s with growth rates superior to those of cattle and poultry. In order to achieve greater productivity, intensive fish cultivation systems are employed, which can cause greater susceptibility to diseases caused by viruses, bacteria, fungi, and parasites. The reduced availability of veterinary medications approved for use in aquaculture in Brazil has lead fish farmers to the indiscriminate use of several chemical substances with antimicrobial activity, such as the dye malachite green (MG). As a result of this use, residues of MG and its main biotransformation product, leucomalachite green (LMG), may be present in fish available for consumption. The presence of residues of these compounds represents a risk to human health due to their toxicity, as well as a potential impact on the environment, and could also raise barriers for commercialization in the country and for exportation. The objective of this review is to provide the context and evidence of the use of MG in aquaculture and of its toxicological and legislative aspects. A review of the analytical methods used to determine MG residues in fish, with emphasis on mass spectrometry, is also presented.     

锗—132对肉用雏鸡免疫功能的影响及其临床应用初探 Ⅲ Ge—132对健康及人工感染vMDV雏鸡某些免疫器官自由基代谢的影响

实验用1日龄肉用雏鸡300只,随机分为四组:Ⅰ组为健康对照;Ⅱ组加喂100PPmGe—132;Ⅲ、Ⅳ组人工感染vMDV,同时Ⅲ组也加喂100PPmGe—132,经60日间临床观察并每隔10日用ESR法测定胸腺、法氏囊及脾组织中自由基含量。测得结果,健康雏龄胸腺、法氏囊及脾组织中自由基平均含量分别为:0.27±0.04、0.34±0.08、0.28±0.06相对浓度(峰/峰),且10～60日龄间无明显变化。通过与健康对照组比较,人工感染vMDV雏鸡各组织中自由基含量均显著增多。Ⅲ组与Ⅳ组对比结果证实,Ge—132能使人工感染vMDV雏鸡免疫器官中自由基含量明显降低。实验结果为阐明自由基与肿瘤病变的发生机理,提供了某些理论依据。