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采取常规检查诊断、生化诊断、病理诊断及水质分析等手段,对101例草鱼肝胆综合症病例的发病原因、症状进行了归纳分析,将患病草鱼病症划分为3个类型。认为长期大量投喂单一精饲料、养殖水体亚硝酸盐含量过高是引起草鱼肝胆综合症的主要原因。提出对草鱼肝胆综合症的诊断应以肝胆形态、颜色及鳃丝炎症作为主要诊断依据,肠道炎症、尾鳍发白及鳃盖充血作为辅助诊断依据。 相似文献
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LI Ning ZHAO Zhi-Hui LIU Zhao-Liang ZHAO Xing-bo LIAN Zhen-xing WU Chang-xin 《中国农业科学(英文版)》2002,1(9):1050-1053
In order to study the expression of function gene and its effect on metabolic control and other physiological function in liver, 438 expressed sequence tags (ESTs) were determined, which were from a cDNA library of porcine liver tissue. The results showed that the nucleotide sequences of 186 ESTs have already presented in GenBank database, and 37 ESTs could be found the homology with human and other species,while the others were not identified. 45 full length insertion of the clones randomly isolated from cDNA library were also completely sequenced with different size, and the results showed that 19 of them were functionknown genes, 11 had no open reading frame ( ORF )at all and 15 had ORF but the function were not elucidated yet. 相似文献
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Dietrich Pizzigatti Fernando Arévalo Batista Charles Ferreira Martins Olímpio Crisóstomo Ribeiro Marcelo Monteiro Nunes Thiago Rinaldi Müller 《Journal of Equine Veterinary Science》2011,31(1):3-7
Both cholangiocarcinoma and squamous cell carcinoma of the stomach stratified squamous epithelial portion are rarely found in equine medicine despite being more common in human beings, dogs, and cats. The objective of the present article was to report the simultaneous occurrences of these two types of neoplasias in an 11-year-old mare. Numerous firm, whitish nodules were distributed throughout the liver parenchyma and those protruding over its surface were umbilicated in their appearance. It was verified that the nodules adhered to the peritoneum and omentum, diaphragm, spleen, and stomach serosa compressed the adjacent structures. The stomach stratified squamous epithelial portion, particularly originating in the margo plicatus toward the cardia, was covered by numerous smooth, whitish spherules. Microscopic examination allowed the liver, tumor, and the abdominal implants to be identified as a cholangiocarcinoma, and the stomach neoplasia as a carcinoma of its stratified squamous epithelial portion. Considering this as an uncommon finding, although when considered individually, the presence of a cholangiocarcinoma and a squamous cell carcinoma of the stratified squamous epithelial portion of the equine stomach in the same specimen is worthy of reporting. 相似文献
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Miao Zhang Lingxiang Xin Endong Bao Jrg Hartung Zhenhua Yue 《Research in veterinary science》2011,90(3):432-438
Twenty pigs were randomly divided into four groups of five pigs each (not transported – control, 1, 2 and 4 h of transportation). A significant increase of ALT, AST and CK in the blood serum and acute parenchyma cell lesions were observed and those were characterized by acute degenerations in the heart and liver. Hsp27 expression levels increased significantly in the heart after 2 h and in the liver after 4 h of transportation, accompanying with the hsp27 mRNA increasing significantly in the heart and liver after 1 h of transportation. αB-crystallin expression levels were fluctuant (not significantly) in the heart and liver during transporting, however, αB-crystallin mRNA increase notably in the heart after 1 h and decrease significantly in the liver at 1 and 2 h of transportation, respectively. In conclusion, the cellular damage to the heart and liver is highest after 1 h of transportation, Hsp27 and αB-crystallin play dissimilar roles and show tissue-specific response in different tissues during transportation. 相似文献
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AIM: To observe the changes of endoplasmic reticulum and the biomarker of endoplasmic retidum stress (ERS), glucose-regulated protein 78(GRP78),in the process of liver fibrosis induced by carbon tetrachloride (CCl4). METHODS: Male Wistar rats weighing 180 g to 220 g were divided into control group and liver fibrosis group. The rats in liver fibrosis group were induced by hypodermic injection of CCl4 for 4 weeks or 8 weeks. The liver index and the serumactivity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were analyzed. The liver fibrosis and the morphological changes of endoplasmic reticulum were observed under light and electronic microscopes, respectively. Additionally, the expression of GRP78 at mRNA and protein levels was determined by real-time PCR and the method of immunohistochemistry, respectively. RESULTS: The liver index, serum ALT and AST activity in liver fibrosis group were obviously higher than those in control group. Swelling and reduced number of endoplasmic reticulum were observed in the hepatocytes of fibrotic rats compare to the controls. The levels of GRP78 protein and GRP78 mRNA in the liver of hepatic fibrotic rats were obviously higher than those in the control rats. CONCLUSION: In the process of liver fibrosis induced by CCl4, the obvious morphological changes of endoplasmic reticulum and increased expression of ERS protein indicate that ERS plays an important role in the liver fibrosis. 相似文献
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AIM: To investigate the expression and prognostic significance of cyclin D1, retinoblastoma-like protein 2(RBL2/p130) and minichromosome maintenance protein 7(MCM7) in hepatocellular carcinoma(HCC). METHODS: The expression of cyclin D1, RBL2/p130 and MCM7 in 44 HCC specimens, 26 adjacent noncancerous cirrhotic liver specimens and 18 normal liver specimens were detected by the method of immunohistochemistry. The correlations of cyclin D1, RBL2/p130 and MCM7 with the clinical parameters of HCC patients were analyzed. RESULTS: The positive expression rates of cyclin D1 and MCM7 in HCC were 68.2% and 72.7%, higher than those in normal livers and adjacent noncancerous cirrhotic livers(P<0.01). The positive expression rate of RBL2/p130 in HCC was 34.1%, lower than that in normal livers and adjacent noncancerous cirrhotic livers(P<0.01). The expression of MCM7 in HCC was positively correlated with the expression of cyclin D1(r=0.349, P<0.05), and negatively correlated with the expression of RBL2/p130(r=-0.421, P<0.01). The expression of cyclin D1 in HCC was negatively correlated with the expression of RBL2/p130(r=-0.435, P<0.01). The expression of MCM7 and cyclin D1 was associated with the integrity of tumor capsule, differentiation degree and TNM stage(P<0.05). The expression of MCM7 was also associated with tumor size and the value of alpha fetoprotein(AFP). The tumor size, the expression levels of MCM7 and cyclin D1 were correlated with the prognosis determined by multivariable analysis. The patients with positive expression of MCM7 and cyclin D1 had lower survival rate than those with negative expression(P<0.05). The patients with positive expression of RBL2/p130 had higher survival rate than those with negative expression(P<0.05). CONCLUSION: The abnormal expression of cyclin D1, RBL2/ p130 and MCM7 plays an important role in the development of HCC, indicating that monitoring their expression in HCC patients may be helpful to the judgment of prognosis. 相似文献