Comparative study of lipoprotein monosaccharide composition between diabetic patients and healthy participants

AIM:To establish a liquid chromatography method for determining the monosaccharide composition of human lipoproteins, and to investigate the differences between diabetic patients and healthy participants. METHODS:Liquid chromatography with pre-column derivatization was used to determine the neutral and basic monosaccharides, and liquid chromatography tandem mass spectrometry was applied to quantify N-acetylneuraminic acid content. RESULTS:The contents of mannose, glucosamine, N-acetylglucosamine, glucose, galactose and N-acetylneuraminic acid in high-density lipoprotein from healthy participants and diabetic patients were (5.88±0.94),(16.49±4.11),(1.31±0.33), (0.87±0.16), (7.18±1.64), (2.14±0.12) mmol/(g protein) and （8.68±0.39), (24.73±5.50), (1.91±0.54), (1.23±0.35), (9.73±2.85), (3.53±0.27） mmol/(g protein）, respectively. The contents of mannose, glucosamine,N-acetylglucosamine, glucose, galactose and N-acetylneuraminic acid in low-density lipoprotein from healthy participants and diabetic patients were (29.20±3.57), (50.77±4.72), (5.28±0.64), (10.06±1.37), （28.44±3.96）,（6.86±0.11) mmol/(g protein) and (30.08±3.78）, （38.52±6.38）, （3.79±0.78）, （7.63±1.50）, （20.05±2.63）, （6.45±0.18) mmol/(g protein), respectively. The contents of mannose, glucosamine, glucose, galactose and N-acetylneuraminic acid in very-low-density lipoprotein from healthy participants and diabetic patients were (91.21±4.12）, （27.05±2.34）, （4 230.95±15.83）, (43.40±3.75), (2.95±0.24) mmol/(g protein) and (82.40±0.51), (30.16±0.32), (4 722.73±93.27), (34.05±2.81), (4.42±0.15) mmol/（g protein）, respectively. CONCLUSION: Liquid chromatography with pre-column derivatization is suitable for the neutral and basic monosaccharide analysis in human lipoproteins, and the glycosylation of lipoproteins in diabetic patients are significantly changed compared with the healthy controls.