Therapeutic effects of recombinant human growth hormone on rat sepsis

AIM:To investigate therapeutic effects of recombinant human growth hormone(rhGH) on rat sepsis and its possible mechanisms.METHODS:Mean arterial pressure (MAP), levels of plasma TNFα, IL-1β and endotoxin, leukocyte count and survival rate within 1 week were determined after E. coli injection among control group, sepsis group and sepsis+rhGH group.RESULTS:(1)rhGH diminished the decrease of MAP, reduced plasma endotoxin and TNFα levels and increased neutrophil ratio in total leukocytes in sepsis rat. rhGH increased survival rate within 1 week on sepsis rat. (2)No changes were found in IL-1β level among the three groups.CONCLUSION:rhGH showed desirable beneficial effects on rat sepsis, which may attribute to: improving circulatory function;maintaining intestinal mucosa barrier, attenuating bacteria/endotoxin translocation and inhibiting the production and release of TNFα.     

哈尔滨沙尘天气成因及其对城市大气污染的影响

文章从空气动力学和天气学角度出发探讨了大风、对流层低层的强烈垂直不稳定及高、低空急流对哈尔滨沙尘天气做出的贡献,分析了沙尘天气时城市大气中几种主要污染物变化情况。研究表明,哈尔滨的沙尘天气主要来源是内蒙古中部的沙地,但近年也常出现本地沙源引起的沙尘天气;沙尘天气期间,城市大气中的可吸入颗粒物浓度出现异常,其浓度变化和沙尘天气具有同步性特征;沙尘天气期间,SO2含量呈明显减弱趋势;NO2污染物的浓度变化则表现较平稳。     

氮沉降对湿地松林土壤呼吸的影响

为了阐明湿地松林土壤呼吸对氮沉降响应的变化规律以及氮沉降是否改变土壤呼吸温度敏感性系数,运用LI-8100开路式土壤碳通量测量系统测定模拟氮沉降4种不同处理水平[0、5、15、30 g/(m2?a)]下湿地松林(Pinus elliottii)的土壤呼吸速率及土壤温、湿度。结果表明：湿地松林土壤胡须呈明显季节变化,7月最高,1月最低。各处理土壤呼吸年累积量为：对照(CK)[5.28±1.02 t/(hm2?a)]、低氮[LN 4.51±0.64 t/(hm2?a)]、中氮[LN 4.66±0.51 t/(hm2?a)]和高氮[HN 4.20±0.43 t/(hm2?a)]。全年对照处理土壤呼吸速率平均值为CK[1.44±0.28 μmol/(m2?s)]比各氮沉降处理LN[1.20±0.17μmol/(m2?s)]、MN[1.24±0.13μmol/(m2?s)]、HN[1.11±0.11μmol/(m2?s)]高16.6%、13.8%、22.9%。土壤呼吸速率与10 cm土壤温度呈显著相关正指数关系(P<0.01),相关性指数(R2)在0.6049~0.7557之间,湿地松林在各处理氮沉降下温度敏感性系数分别为LN(1.72)、MN(1.75)、HN(1.79)同对照(2.35)相比有降低趋势。土壤呼吸与5 cm土壤湿度之间呈二次方程关系,但相关性不显著(P>0.05)。研究表明,氮沉降是影响湿地松林CO2通量的一个重要因子。     

烟草PR10蛋白生物活性及赤星病菌Alternaria alternata诱导下的表达分析

为深入研究植物PR10蛋白的生物学功能,以烟草PR10蛋白(NtPR10)为研究对象,采用冷休克蛋白表达载体p Cold II,构建烟草PR10融合蛋白原核表达系统,优化表达及纯化条件,获得高纯度NtPR10融合蛋白;分别采用底物法和滤纸片法体外分析其核酸酶活性及抑菌活性;并利用荧光定量PCR方法分析了烟草赤星病菌Alternaria alternata侵染下,NtPR10基因在抗、感烟草品种内不同时间点的表达差异。结果表明,15℃、0.1 mmol/L IPTG过夜条件下可诱导获得大量可溶性目的蛋白,50、100 mmol/L咪唑缓冲液洗脱能够获得较高纯度的目的蛋白;纯化后的NtPR10蛋白能够降解烟草总RNA,具有核酸酶活性,且1.0、0.5、0.25μg/μL的NtPR10蛋白溶液均对烟草赤星病菌的菌丝生长具有显著的抑制作用,但随着蛋白浓度的降低,抑菌作用减弱;荧光定量PCR结果显示,接种烟草赤星病菌后,NtPR10基因在感病品种和抗病品种中均显著上调表达,但其在抗病品种的响应速度和表达量显著高于感病品种,表明NtPR10应答了烟草赤星病菌的侵染过程。     

Role of nuclear factor κB on the expression of interleukin-6 in mouse mesangial cells induced by interleukin-1β

AIM:To investigate the regulatory role of nuclear factor κB (NF-κB) in the expression of interleukin-6 in mesangial cells (MC) induced by interleukin-1β.METHODS:Activation of NF-κB was measured by electrophoresis mobility shift assay (EMSA). RT/PCR and ELISA were used to detect IL-6 mRNA expression and IL-6 production, respectively.RESULTS:rhIL-1β could rapidly stimulate the activation of NF-κB in MC, and increase the expression of IL-6 mRNA and protein. PDTC, one of the inhibitor of NF-κB, could inhibit the expression of IL-6 in mRNA and protein in MC stimulated by rhIL-1β.CONCLUSION:IL-6 expression induced by IL-1β may be regulated by NF-κB in MC, NF-κB may modulate the immune-inflammatory reaction in glomerular disease.     

结核分枝杆菌CFP10-ESAT6融合蛋白的真核表达及其牛结核病诊断价值评价

本研究旨在利用毕赤酵母(Pichia pastoris)系统融合表达结核分枝杆菌(Mycobacterium tuberculosis,MTB)的培养滤液蛋白10(culture filter protein 10,CFP10)和早期分泌抗原靶6蛋白(earlier secreted antigen target 6,ESAT6),并评价其作为牛结核病外周血γ-干扰素(interferon gamma,IFN-γ)体外释放实验特异性刺激剂来诊断牛结核病的应用潜能.通过PCR扩增cfp1 0-esat6融合基因,构建pPICZα A-(cfp10-esat6)重组质粒,电转化毕赤酵母GSll5,添加甲醇至终浓度1.0％,诱导3d,取培养上清进行SDS-PAGE分析,镍离子金属螯合亲和层析柱纯化目的蛋白,Western blot分析重组蛋白的免疫反应性;取纯化的融合蛋白CFP10-ESAT6作为刺激剂用于牛结核病外周血IFN-γ体外释放实验,评价其牛结核病诊断价值.结果表明,目的蛋白(33kD)被成功分泌表达,该融合蛋白与抗CFP10、ESAT6、组氨酸标签和c-Myc4种单抗均发生特异性反应,具有较好的免疫反应性.165份奶牛外周血样品的IFN-γ检测结果表明,CFP 10-ESAT6融合蛋白与结核菌素纯蛋白衍生物(purified protein derivative,PPD)作为刺激剂,二者阳性符合率为82.3％,阴性符合率为78.8％.本研究利用酵母表达系统成功表达CFP10-ESAT6融合蛋白,并表现出更高的生物学活性,在牛结核病外周血IFN-γ体外释放实验中可作为候选刺激剂,并能克服混合感染导致的PPD漏检问题,从而进一步提高检测的敏感性和特异性.     

基于AOD数据的新疆大型露天煤炭开采区PM2.5和PM10反演

MODIS气溶胶产品AOD与PM2.5、PM10浓度高度相关,已广泛应用在PM2.5、PM10浓度模拟.该研究以新疆维吾尔自治区大型露天煤炭开采区准东矿区为研究对象,结合实测的2014年5月、7月、9月、12月PM2.5、PM10质量浓度数据与经过垂直湿度订正的MODIS气溶胶产品AOD,利用多元回归进行拟合建模,从建立的40个模型中选取最优模型并据此对研究区PM2.5、PM10的质量浓度进行定量估算.结果表明:AOD与PM2.5、PM10呈极显著正相关;4个月AOD与PM2.5、PM10质量浓度估算模型最优模型均为多项式模型;其中7月AOD与PM2.5质量浓度拟合模型较好(R2=0.6258),实测值与预测值拟合趋势线R2为0.8057;9月PM10拟合模型效果理想(R2=0.7329),实测值与预测值拟合趋势线R2为0.8077;将AOD代入最优模型反演PM2.5,从空间层面上反映出各区域PM2.5浓度差异明显.研究结果可为AOD的深度利用与PM2.5、PM10浓度的遥感估算提供参考,在大气污染物空间分布、监测大气环境质量、污染预测等方面都具有重要意义.     

The expression of IFN-γ and IL-4 on T lymphocytes that infiltrate in nasal polyps

AIM:To investigate the expression of Th1-typed cytokine IFN-γ and Th2-typed cytokine IL-4 on T lymphocytes that infiltrate in nasal polyps for searching the pathogenesis of nasal polyps. METHODS:Nasal polyps tissue samples and peripheral blood were obtained from 21 patients. Normal human inferior turbinate mucosa and peripheral blood were obtained as well. Flow cytometry was adopted to detect the expression of IFN-γ and IL-4 of T lymphocytes. RESULTS: Th cytokines were rarely detected in inferior turbinate from normal human. Nasal polyps tissue consisted of abundant T lymphocytes. The expression of IL-4 and IFN-γ increased in peripheral blood from patients compared with normal human (P<0.05). The expression of IL-4 increased but the expression of IFN-γ decreased in nasal polyps compared with that of peripheral blood from the same patient (P＜0.05). CONCLUSION:There were generous of T lymphocytes infiltrating in nasal polyps. There was abnormal immune status in the local nasal mucosa from the patients, and the predomination of Th cytokine secretion changed compared with peripheral blood from the same patients, which resulted in the change of microenvironment of nasal mucosa and possibly close related to the formation of nasal polyps.     

长白山典型阔叶红松林土壤二氧化碳排放通量

随着大气CO2浓度的升高,主要由其引起的温室效应与对生物新陈代谢的影响变得越来越显著。森林生态系统在全球碳循环中扮演着重要的角色。为了评估和理解森林土壤CO2通量及其随空气和土壤温度的季节和昼夜变化规律,我们在长白山北坡典型阔叶红松林内利用静态箱技术进行了原位观测。实验在整个生长季(6月初至9月末)昼夜进行,利用气相色谱进行气体分析。结果表明: 长白山阔叶红松林土壤是大气二氧化碳源,其CO2通量具有明显的季节和昼夜变化规律。通量的变化范围是(0.30-2.42)μmol穖-2穝-1,平均值为0.98μmol穖-2穝-1。土壤CO2排放的季节规律表明,土壤CO2通量的变化与气温和土壤温度的变化有关。CO2平均通量的最大值出现在7月((1.27±23%)μmol穖-2穝-1),最小值出现在9月((0.5±28%)μmol穖-2穝-1)。土壤CO2的昼夜波动与土壤温度变化有关,而在时间上滞后于温度的变化。森林下垫面土壤CO2通量与土壤温度显著相关,与6cm深度土层温度相关系数最大。基于气温和土壤温度计算的Q10值范围为2.09-3.40。图2表3参37。     

Alteration of serum interleukin-6 and tumor necrosis factor-α after ischemic stimulation of coronary artery in PTCA

AIM: Inflammatory responses play an important role in the post- percutaneous transluminal coronary angioplasty (PTCA) restenosis and has been demonstrated occuring immediately after PTCA. Interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) are the main inflammatory cytokines. We try to compare the changes in interleukin-6(IL-6) and TNF-α after PTCA in the patients with and without collateral circulation to probe into the pathogenesis of early inflammatory response. METHODS: The extent of myocardial ischemia induced by balloon inflation was quantified by a scoring system referring to the Leaman coronary score. The IL-6、TNF-α levels of coronary heart disease group and control group before and after PTCA are calculated. RESULTS: The concentrations of IL-6 and TNF-α were (9.592±1.847) ng/L and (26.959±1.967) ng/L, respectively, and were significantly increased 4 hours after PTCA. CONCLUSION: IL-6 and TNF-α are sensitive indicators of the early inflammatory response after PTCA. Ischemia scores reflected the extent of ischemia reperfusion injury during PTCA. Collateral circulation decreased the early inflammatory response after PTCA.