The mechanism of apoptosis induced by homoharringtonine in HL-60 cells

AIM: To study the signal transduction pathway of apoptosis initiation induced by homoharringtonine in HL-60 cells. METHODS: After establishing the model of apoptosis initiation induced by homoharringtonine in HL-60 cells, at the point of apoptosis initiation, molecular caspase-3, Bcl-2, Bax and Fas/FasL were measured with flow cytometry and transmission electron microscope. ERK2 and P38 expression in HL-60 cells were detected by using immunohistochemistry. RESULTS: The model of apoptosis initiation induced by homoharringtonine was established in HL-60 cells. At the point of apoptosis initiation, upregulation of caspase-3 and decrease in Bcl-2/Bax were observed. However, the expression of Fas/FasL did not significantly change. ERK2 expression decreased and P38 expression increased. CONCLUSIONS: Caspase-3, Bcl-2, Bax and mitogen activated protein kinase pathways were involved in signal transduction of apoptosis initiation induced by homoharringtonine in HL-60 cells.     

植物Ca2+信号的研究进展

在植物的生长发育过程中,为了适应环境,调节自身代谢和生长,需要对各种外界环境刺激以及植物内部生理信息做出反应,因此,植物产生了自己的信号系统。Ca2 作为一种信号分子在植物细胞信号系统中起着举足轻重的作用。针对国内外对植物Ca2 信号的研究情况,综述了Ca2 信号的产生、Ca2 信号参与的各种植物生理过程、Ca2 信号的检测以及其研究的最新进展。     

德南牛全基因组变异解析和功能基因挖掘

[目的]研究德南牛的群体遗传结构和解析影响其重要性状的关键基因。[方法]通过主成分分析、群体进化树分析、群体遗传结构分析等方法,研究德南牛的遗传结构和与其他品种的亲缘关系;同时,利用全基因组SNP数据,计算德南牛与德国黄牛、南阳牛群体固定指数(Fst)和核苷酸多样性比值(π ratio)、复合似然比(CLR)、XP-EHH等多种选择信号指标,以筛选出德南牛基因组上受到选择的信号,鉴定出重要的候选基因。[结果] 德南牛具有欧洲普通牛(0.789)、东亚牛(0.176)、中国瘤牛(0.035)的混合血统。在θπ和CLR方法中鉴定出93个共有基因,使用FST和XP-EHH方法鉴定出17个共有基因。进行富集以及基因功能注释后,发现这些基因可能与生殖性能(TUBB3、NSD2、GRM1、ERBB4)、生长性状(RFX3、FGFR3、GABRB1、PDE4D)、环境适应性(PGR、GRIA4)和免疫系统反应(P2RY12、P2RY13、GPR87、P2RY14、GPR171)有关。[结论]本项研究发现德南牛血统受德国黄牛影响较多,且挖掘了德南牛基因组内可能具有经济意义的性状相关的选择特征,为德南牛种群的选育提高提供理论支撑。     

机械故障诊断技术研究综述

随着国民经济的飞跃发展和人们日益增长的生活需求,机械系统越来越复杂,功能越来越完善。同时,鉴于各行业对机械使用的密切性和广泛性,机械故障的发生和检测诊断技术也引起了各国的关注和研究。在机械中,旋转机械是使用最广泛的,几乎每个机械系统中都有旋转部件的存在,因此,对旋转机械故障的检测和诊断技术的研究也备受关注。基于此,从振动信号处理技术和图像处理技术对国内外故障检测和诊断的研究进行分析,以及对故障检测诊断领域的发展方向进行展望。     

CYP450 epoxygenase/EET pathway attenuates adipose inflammation of obese mice through HIF-1α

AIM To investigate the effects of cytochrome P450 （CYP450） epoxygenase/epoxyeicosatrienoic acid （EET） pathway on insulin resistance in obese mice, and to explore the possible mechanisms. METHODS High-fat diet-induced obesity model was established in C57BL/6Cnc mice, and the obese mice were randomly divided into 3 groups, including obesity group （treated with saline; n=10）, EET group （treated with 11,12-EET; n=10） and EET inhibitor 14,15-epoxyeicosa-5（Z）-enoic acid （EEZE） group （n=10）. Normal C57BL/6Cnc mice （n=10） treated with saline served as control. Protein expression of CYP2J2 （one of CYP450 epoxygenases） and hypoxia-inducible factor-1α （HIF-1α） was measured by Western blot. Vessel-like structure was detected by immunofluorescence staining. The serum levels of insulin, tumor necrosis factor-α （TNF-α）, interleukin （IL）-1β, IL-6 and monocyte chemoattractant protein-1 （MCP-1） were measured by ELISA. RESULTS In obese mice, homeostasis model assessment of insulin resistance （HOMA-IR） values were increased, the protein level of CYP2J2 was reduced, and the protein level of HIF-1α was increased in adipose tissues as compared with the controls （P<0.05）. The serum levels of MCP-1, IL-1β, IL-6 and TNF-α were also significantly increased in obese mice （P<0.05）. After treatment with 11, 12-EET, the HOMA-IR values were decreased compared with vehicle-treated obese mice, HIF-1α expression levels were decreased in the adipose tissue, and the serum levels of MCP-1, IL-1β, IL-6 and TNF-α were reduced （P<0.05）. Immunohistochemical results of adipose tissue from vehicle-treated obese mice showed a marked decrease in vessel-like structures （CD31-positive） compared with normal control mice （P<0.05）. EET treatment significantly increased the newly formed vessel-like structures in the visceral adipose tissues of obese mice as compared with vehicle-treated obese mice （P<0.05）. CONCLUSION High-fat diet-induced obesity and insulin resistance are closely related to the CYP450 pathway. Exogenous EETs effectively decrease obesity-induced insulin resistance possibly through pro-angiogenesis and attenuation of hypoxia and inflammation.     

茉莉酸甲酯(MJ)对植物抗性信号转导的诱导

茉莉酸甲酯(MJ)可以通过多种途径诱导植物对病虫害产生抗性,综述了近年来MJ诱导植物防御反应的信号转导途径的研究进展。     

基于混沌的超声物位检测系统的研究

物位检测技术在现代化工业控制中占有重要地位。由于现场具体的安装环境不同,被测介质种类和状态不同,情况异常复杂,因此必须根据具体的现场工况,合理地选择物位检测的方法和产品。为此,提出了一种基于混沌理论的超声波物位检测系统,并论述了信号处理方法,从而能准确识别噪声干扰与超声波回波信号,提高了物位检测精度。     

铣削刀具不同磨损期振动信号的分维特征

提出了应用铣削刀具磨损工作状态下的振动加速度信号的关联维数来描述刀具不同磨损状态下的特征,进而实现对状态的监测、识别。试验证明,刀具磨损在相同状态下,振动信号具有相近的关联维数,在不同状态下则有明显的关联维数。因此,关联维数不仅可以作为状态监测与识别的重要依据,而且可以作为其他特征提取方法的补充。     

GPX4失活通过JNK通路缓解LPS诱导巨噬细胞炎症反应

【目的】研究硒蛋白谷胱甘肽过氧化物酶4(glutathione peroxidases 4,GPX4)失活如何参与调控脂多糖(lipopolysaccharide, LPS)诱导的RAW264.7巨噬细胞炎症反应及其潜在的分子机制。【方法】体外培养RAW264.7巨噬细胞,以DMSO为对照,使用0.1～5.0μmol/L GPX4抑制剂FIN56处理,通过CCK-8法检测细胞活力和Western blotting检测GPX4蛋白表达水平,确定抑制剂最适浓度。将RAW264.7巨噬细胞分为4组：对照组,添加DMSO培养24 h; FIN56(GPX4抑制剂)组,添加0.5μmol/L FIN56培养24 h; DMSO-LPS组,DMSO培养24 h后使用LPS(100 ng/mL)刺激3 h; FIN56-LPS组,FIN56培养24 h后使用LPS刺激3 h。各组细胞经培养后,利用荧光探针2′,7′-二氯二氢荧光素二乙酸酯(2′,7′-dichlorodi-hydrofluorescein diacetate, H₂DCFDA)检测细胞内活性氧(reactive...     

新疆黑蜂基因组选择信号分析

【目的】分析新疆黑蜂(Xinjiang Black bee)与4个引进西方蜜蜂品种间的遗传进化关系,并通过检测基因组选择信号,发掘新疆黑蜂重要种质特性相关的候选基因。【方法】对新疆黑蜂、意大利蜜蜂(Apis mellifera ligustica)、高加索蜂(Apis mellifera caucasica)、卡尼鄂拉蜂(Apis mellifera carnica)、欧洲黑蜂(Apis mellifera mellifera)共5个蜂种50个蜂王个体和1个长白山中华蜜蜂蜂王(Apis cerana cerana)样本进行全基因组重测序数据分析,鉴定新疆黑蜂的单核苷酸多态性(single nucleotide polymorphism, SNP)标记,以长白山中华蜜蜂为外群利用邻接法构建系统进化树以阐明新疆黑蜂的进化关系,利用SNP信息进行新疆黑蜂的主成分分析、群体遗传结构分析及连锁不平衡分析;采用群体遗传分化指数(Fst)和核苷酸多样性比值(θπ)方法检测新疆黑蜂与其他西方蜜蜂群体间的选择信号,并对提取到的受选择区域候选基因进行GO功能和KEGG通路富集分析。【结果】在新疆黑蜂中共鉴...