湘红菜薹一号的选育

湘红菜薹一号是以自交不亲和系９４１５－２和孤雌生殖系Ｊ－７－２配制的紫菜薹一代杂种,极早熟,从播种到始收４５天左右,早期产量高,菜薹亮紫色,无蜡粉,味甜,品质好,耐热,抗软腐病能力强,适应性广,适用于长江流域夏秋栽培和早熟秋冬栽培。     

大叶芥菜作为植物熏蒸材料与主栽作物番茄间作模式探索

以大叶芥菜为主要的植物熏蒸材料,通过改变其与主栽作物番茄间作的不同种植模式,分析大叶芥菜产量、大叶芥菜硫代葡萄糖苷含量、番茄根结指数、番茄产量等指标,探索生物熏蒸效果佳、作物产量高、可以大面积推广的间作模式。结果表明:间作模式1(栽培大叶芥菜前将番茄吊蔓的2架钢丝距离调整为50~60 cm,第1茬番茄拉秧后将棚内温度调高2~5 ℃)效果最好,大叶芥菜的产量、硫代葡萄糖苷含量最高,根结线虫抑制效果最好,同时每667 m^2番茄产量最高,达12 216.5 kg,可大面积推广。     

Effects of exosomes derived from hypoxia-preconditioned umbilical cord mesenchymal stem cells on function of endothelial cells

AIM To investigate the effect of exosomes derived from hypoxia-preconditioned human umbilical cord mesenchymal stem cells (hUCMSCs) on proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs). METHODS hUCMSCs and HUVECs were isolated, cultured and identified. Exosomes derived from hUCMSCs were extracted by ultracentrifugation. The morphological change of exosomes was observed under transmission electron microscope. The particle size and concentration of exosomes were detected by nanoparticle tracking analysis, and the surface specific marker proteins of exosomes were determined by Western blot. hUCMSCs were divided into normoxia group and hypoxia group. The viability of hUCMSCs was measured by CCK-8 assay. HUVECs were divided into control group, normoxic exosome group and hypoxic exosome group. The proliferation of HUVECs was detected by EdU assay. The migration ability was detected by cell scratch assay and Transwell experiment. Tube formation ability was evaluated by tube formation experiment. RESULTS Compared with normoxia group, hypoxia pretreatment enhanced the viability and exosome release of hUCMSCs. Compared with normoxic exosome group, hypoxic exosomes enhanced the proliferation, migration and tube formation of HUVECs. CONCLUSION Exosomes derived from hUCMSCs under hypoxia enhances the proliferation, migration and tube formation of HUVECs.     

云南苹果产区苹果茎沟病毒(ASGV)的发现及其分子变异

【目的】为了查明云南省苹果产区的苹果茎沟病毒病毒(Apple stem grooving virus,ASGV)的侵染情况和不同来源分离物间的分子变异情况,【方法】以云南昭通、丽江、昆明、曲靖等地采集的325份苹果病毒样品为试材,采用RT-PCR技术对其是否携带ASGV进行检测,运用DNAMAN6.0、MEGA5.0对实验获得的6个分离物和GenBank上已登录的34个分离物进行序列比对分析。【结果】ASGV在云南各大苹果产区的发病率高达48%。序列比对结果显示,这些分离物的核苷酸序列相似性为87.3%～93.8%,氨基酸序列相似性为90.7%～98.7%。系统发育树显示,云南的6个分离物聚为一簇,而陕西省苹果分离物YL06与云南省分离物ZT1、TJX1亲缘关系比较近,聚为一支;ASGV苹果分离物要明显区别于ASGV梨分离物和CTLV柑橘分离物。【结论】云南省苹果产区苹果树的ASGV带毒率高达48%,推测可能是一种发生率比较普遍的病毒病,这是云南省发现的首次报道。系统发育树显示:本研究获得的6个苹果分离物明显区别于其他寄主分离物,并且与其他地区的分离物具有一定差异,推测在我国,ASGV的分子变异与寄主/地域来源存在着一定的相关性。     

鹿茸干细胞体外培养技术的研究

利用消化酶消化法,用DMEM培养基对鹿茸干细胞进行了体外培养,分别从原代培养、传代、冻存、以及复苏等几个环节进行了试验。摸索鹿茸干细胞合适的体外培养条件,为鹿茸的各项相关研究打基础。结果表明,用透明质酸酶和胶原酶对组织样进行消化,然后用10%DMEM培养基进行培养细胞生长效果较好。适宜培养条件是37℃、5%CO2最大饱和湿度。     

乐至黑山羊与营山黑山羊杂交改良效果初探

本试验利用乐至黑山羊(父本)杂交改良营山黑山羊(母本)以提高生产性能。分别测定了两品种羊及乐×营杂交一代羊6月龄的体高、体长、胸围、胸宽和管围以及2月龄、6月龄、12月龄和24月龄的体重,结果表明:杂交一代羊的所有指标明显高于营山黑山羊(P0.05),其中6月龄杂交一代公羊的体高、体长、体重、胸围和胸宽较营山黑山羊分别提高23.3%、25.2%、18.39%、38.9%和27.7%(P0.01);母羊体高、体长、体重、胸围和管围分别提高19.3%、21.7%、22.72%、26.4%和13%(P0.01),胸宽提高6.2%(P0.05)。说明杂交改良效果显著。     

蓝白花肉牛杂交改良本地黄牛的效果

2002--2004年化隆县引进蓝白花肉牛细管冻精1000支对本地黄牛进行改良,共授配本地黄牛416头,生产蓝本F1353头。同时测定了蓝本F1初生、6月龄和12月龄时的体重、体长、胸围和体斜长指标,并与本地黄牛进行了比较。结果表明,蓝本F1表现出了较强的杂种优势,杂交效果显著。     

Expression of receptor tyrosine kinases VEGFR-1 (FLT-1), VEGFR-2 (KDR), EGFR-1, PDGFRa and c-Met in canine primary brain tumours

Inhibition of tumour growth and angiogenesis by targeting key growth factor receptors is a promising therapeutic strategy for central nervous system tumours. Characterization of these growth factor receptors in canine primary brain tumours has not been done. Using quantitative real‐time TaqMan polymerase chain reaction (PCR), we evaluated the expression of messenger RNA (mRNA) for five tyrosine kinase growth factor receptors (vascular endothelial growth factor receptor [VEGFR]‐1, VEGFR‐2, endothelial growth factor receptor [EGFR]‐1, platelet‐derived growth factor receptor a [PDGFRa], and c‐Met) relative to normal cerebral cortex in 66 spontaneous canine primary brain tumours. Increased expression of VEGFR‐1 and VEGFR‐2 mRNA was greatest in grade IV astrocytomas (glioblastoma multiforme) and grade III (anaplastic) oligodendrogliomas. EGFR‐1 mRNA expression was more consistently increased than the other receptors in all tumour types, while increased PDGFRa mRNA expression was mostly restricted to oligodendrogliomas. The similarities in increased expression of these tyrosine kinase growth factor receptors in these canine tumours, as compared to data from their human counterparts, suggest that common molecular mechanisms may be present.     

基于CO I基因序列的11种蛀茎害虫的分子鉴定

本研究克隆了二化螟(Chilo suppressalis)、台湾稻螟(Cauricilius)、芦苞螟(C.luteellus)、甘蔗条螟(C.sac-chariphagus)、三化螟(Tryporyza incertulas)、亚洲玉米螟(Ostrinia furnacalis)、甘蔗红尾白螟(Scirpophaga ex-cerptalis)、黄纹髓草螟(Calamotrophapaludella)、桃蛀螟(Conogethes puncti eralis)、棘禾草螟(Chilo hyrax)和稻蛀茎夜蛾(Sesamia in.erens)11种常见蛀茎害虫102个个体的线粒体DNA细胞色素氧化酶亚基Ⅰ(COⅠ)基因片段.序列分析显示:该COⅠ基因片段长为709 bp,序列都未发生缺失或插入现象,碱基颠换率(55.42％)高于转换率(44.58％);种间遗传距离平均值为0.140(0.088～0.179),种内遗传距离平均值为0.004(0～0.015),两者之间没有重叠区域;聚类分析表明,不同种蛀茎害虫分别形成独立的进化分支,分支自展值均为100％.研究结果表明该COⅠ基因序列具有适宜的变异信息,种内相对保守,种间变异显著,适用于蛀茎害虫的物种识别,并为开发蛀茎害虫DNA条形码技术奠定了基础.     

应用缩节安(DPC)调控棉花株型的定位定量效应研究

缩节安(1,1-dimeyl piperidinium cloride, DPC)是棉花生产中广泛应用的植物生长延缓剂,其调控棉花茎枝生长的定位定量效应尚缺乏系统的量化研究。本研究 2013-2014 年在田间条件下分别于棉花现蕾期、盛蕾期后、盛花期前、盛花期后和打顶后单次应用不同剂量的 DPC,测量了 DPC 作用有效期内的棉花株高和主茎生长速率,探究了所有主茎节间及所有果节对 DPC 的响应。结果表明, DPC 处理对棉花主茎节间的影响范围为 N 节(应用 DPC 时的主茎节)以下 1~4 个和 N 节以上 0~6 个(打顶条件下),对果枝的影响范围为 N 节以下 1~11 个和 N 节以上 0~5 个,其中 N 节以下果枝受影响的果节多于 N 节以上果枝。将盛蕾期后和盛花期前 2 次应用 DPC 的效应叠加,其影响范围几乎可以覆盖全部主茎节间(果枝始节以上)和全部果节。DPC 应用剂量与其作用强度并不总存在较好的线性关系。DPC 的定位定量效应除了与应用时间和剂量有关,还受到温度、降水等环境条件和棉株生物量、源库关系的影响。