Structure and Expression In planta of Botrytis cinerea Ubiquitin Genes

To identify genes of the necrotrophic pathogenic fungus Botrytis cinerea that are expressed during infection of tomato leaves, a differential screening of a genomic library with radioactively labelled cDNA was performed. This resulted in the identification of a B. cinerea gene, denominated Bcubi4, which encodes a precursor protein consisting of four identical head-to-tail repeats of a 76aa ubiquitin unit. Subsequently a gene denominated Bcubi1CEP79, encoding a single ubiquitin unit joined to a Carboxyl Extension Protein of 79 amino acids, was isolated. The expression of the two ubiquitin genes was studied during pathogenesis of B. cinerea on tomato. Bcubi1CEP79, but not Bcubi4, mRNA was transiently induced at 16h after inoculation. The increased expression of the Bcubi1CEP79 gene at this stage of pathogenesis might be required for enhanced ribosomal biogenesis.     

不同饲用燕麦品种生产性能的综合评价

基于熵权赋权法,运用灰色系统理论中的灰色关联度法对26个燕麦品种的生产性能进行综合评价,结果表明,生产性能综合表现较好的是初岛燕麦、青永久409、青永久709及青永久316;青永久18综合评价值最低,生产性能最差,其余品种生产性能表现一般。经逐步回归分析,发现其中的绿叶数、叶长、茎叶比、鲜干比、生长速度、叶面积指数、相对生长速率及生长天数8个指标对饲用燕麦生产性能贡献显著。     

Chitosan Stimulates Defense Reactions in Grapevine Leaves and Inhibits Development of Botrytis Cinerea

Chitosan (β-1,4-linked glucosamine oligomer) derived from crab shells conferred a high protection of grapevine leaves against grey mould caused by Botrytis cinerea. Under controlled conditions, it was shown to be an efficient elicitor of some defense reactions in grapevine leaves and to inhibit directly the in vitro development of B. cinerea. Treatment of grapevine leaves by chitosan led to marked induction of lipoxygenase (LOX), phenylalanine ammonia-lyase (PAL) and chitinase activities, three markers of plant defense responses. Dose-response curves show that maximum defense reactions (PAL and chitinase activities) and strong reduction of B. cinerea infection were achieved with 75–150 mg l⁻¹ chitosan. However, greater concentrations of chitosan did not protect grapevine leaves with the same efficiency, but inhibited mycelial growth in vitro. Present results underlined the potency of chitosan in inducing some defense responses in grapevine leaves which in turn might improve resistance to grey mould.     

冷冲模设计课程的教学改革思考

冷冲模设计课程的教学,既要重视理论知识的教授,又要重视专业实践教学.为培养专业技能强、综合素质高的模具设计人才,必须改革冷冲模设计课程教学模式,重点要对课程的教学内容、教学方法、实践教学及教学考核等进行调整与改造,完善整个课程的教学体系.     

一种驻车制动器固定座切边、冲孔复合模

介绍了驻车制动器固定座切边、冲孔模具的结构,分析了在成型中的受力情况,提出了模具结构设计方法,为驻车制动器固定座的成形问题提供了一种解决方案。     

桔皮提取物抑制霉菌试验

试验选用乙醚，丙酮，乙醇３种溶剂提取桔皮中有效成分，分别制成６０％的乳剂，用灭菌蒸馏水稀释成不同浓度梯度，加入培养其中进行抑制霉菌试验。     

栗果霉烂机理及防治

为探讨栗果贮藏、运输期间的霉烂机理，通过大量调查研究，提出了影响栗果霉烂的主要原因及防治方法，为提高栗果的质量和保鲜度提供科学的理论依据。     

聚氨酯橡胶胀形模设计

在生产中大型制件胀形模,由于材料拉伸变形较大,往往导致制件开裂、起皱等问题.小型制件采用聚氨酯橡胶制造胀形模已得到了应用,以此为基础,对摩托车油箱这种较大零件的胀形模进行改进,用聚氨酯橡胶代替原钢制凸模,很好地解决了零件出现的缺陷,在生产中已获得了成功并投入使用.     

模糊控制在纸浆模塑包装容器生产环节中的实现

纸浆模塑生产过程是一个典型的多变量、强耦舍、多干扰、时变复杂的系统。根据其控制过程中被控对象复杂而难以控制的特点。采用模糊控制策略构建了纸浆模塑包装容器生产环节中的软、硬件系统。     

橡胶木的防变色试验

通过不同浓度的不同防变色剂分别对未处理和经过硼防腐剂和Parachem防腐剂加压处理的橡胶木锯材进行浸沾处理，对其防变色效果进行了研究。结果表明，无论经过硼防腐剂处理与否，用防变色剂进行短时间（30s)的浸沾处理，均可有效地防止橡胶木的蓝变和发霉，保护期可达4周以上。