香石竹组培变态苗的酯酶和过氧化物酶的研究

本文对香石竹正常组培苗和变态苗的过氧化物酶和酯酶活性及其同工酶进行比较研究。结果表明变态苗的这两种酶活性变化不大 ,但茎叶两器官均有过氧化物酶和酯酶同工酶丢失现象。外部形态上的变态现象同分子水平上酶的变化密切相关。     

芝麻苗期干旱生理生化指标响应特征及其基因表达分析

为建立芝麻抗旱性快速鉴定体系及筛选芝麻抗旱品种，采用盆栽反复干旱法，设置正常水分（CK）和干旱胁迫（DS）两种处理，对31份芝麻材料进行生理生化指标测定和综合评价，同时利用qRT-PCR检测SOD合成相关基因的表达量。结果表明：芝麻苗期O^₂、可溶性糖（SS）、脯氨酸（Pro）含量和超氧化物歧化酶（SOD）、过氧化物酶（POD）活性与CK相比均显著上升，各指标综合抗旱系数和抗旱指数的变异系数最高的分别是SOD（98.64%）和O^₂（154.01%）；抗旱指数与O^₂含量、SOD活性呈极显著正相关关系，这两个指标可作为芝麻苗期抗旱性鉴定的重要指标。聚类分析将31份芝麻材料划分为5类抗旱类型，分别为高抗型、中抗型、低抗型、低感型和高感型。利用综合评价方法，筛选出高抗材料2份（‘汾芝10号’和‘豫-11-1’），中抗旱材料4份，低抗旱材料9份，敏感材料10份，高感材料5份；筛选出O^₂含量和SOD活性可作为芝麻种质资源苗期抗旱特性快速鉴定的指标。     

In vitro metabolism of ovarian steroids by bovine whole blood and plasma

Experiments were conducted to evaluate the effects of time and temperature on the potential of bovine whole blood (WB) or plasma (PL) to metabolize the ovarian steroids progesterone, estradiol-17β and testosterone. During a radioimmunoassay study (Experiment 1), we observed a temperature and time-dependent reduction (P<0.001) of plasma progesterone concentrations in samples incubated as WB at 5, 15, 25, or 35C for up to 48 hr. Most notable was the observation that 27% of progesterone present in controls was lost when WB was incubated at 5C for 48 hr and a 17% reduction was observed when PL samples were incubated at 35C for 48 hr. Immunoreactive estradiol-17β concentrations (Experiment 2) in PL and WB incubates were not affected by time or temperature. However, immunoreactive testosterone concentrations increased more than 3-fold by 48 hr in WB incubates held at 35C. To examine the latter observation further, ³H-progesteone was incubated with WB at 35C, followed by extraction and thin-layer chromatography (Experiment 3). Results generally supported RIA findings and revealed the presence of significant 17α-hydroxylase, 17–20 lyase and aromatase activity. Heretofore this has not been considered to occur outside major steroid metabolizing organs.     

辽宁省不同地区的反枝苋对氯嘧磺隆抗药性研究

在辽宁省不同地区的大豆田中采集反枝苋种子,在室内通过琼脂法和茎叶处理法,测定辽宁不同地区反枝苋对氯嘧磺隆的抗性水平差异以及对反枝苋幼苗的影响。试验结果表明:铁岭开源地区的反枝苋,相对抗性比为751;锦州北镇和丹东东港的反枝苋,其相对抗性比分别为294.5、167;不同地区反枝苋敏感性种群和抗性种群幼苗的苗高、根长、植株鲜重的相对毒力倍数和抗性指数均存在一定的差异。     

水生美人蕉组培快繁技术研究

以水生美人蕉带有芽苞的根茎为外植体进行组培快繁技术研究,结果表明:MS BA1.0～4.0mg/L NAA0.2mg/L培养基能较好地诱导分化出不定芽;MS BA6.0～8.0mg/L NAA0.2mg/L培养基增殖效果最好;在MS不加激素和MS NAA0.2mg/L培养基上进行生根培养都有不同程度的根分化。株高6～8cm出瓶用苔藓炼苗,成活率达95%以上。     

大叶速生槐种根育苗技术研究

[目的]提高大叶速生槐种根的繁殖系数和苗木的质量。[方法]对不同直径不同长度的大叶速生槐种根的繁殖方法进行研究。[结果]直径5 mm以上的种苗种根采用直接栽种的方法,直径3～5 mm的种根采用先育苗再移栽的方法,育苗主要采用沙坑密植、营养袋育苗和塑管育苗等方法。[结论]此法不仅提高了大叶速生槐种根的繁殖系数,而且提高了苗木的质量。     

家蚕品种茧层生产效率的调查及相关分析

251个家蚕品种的茧层生产效率平均为10．11％，最高12．19％，最低6．71％。二化性品种较高，一化性和多化性较低。中系二化种最高，平均10．54％，日二化种次之，平均10．39％，欧一化平均9.08％，日一化8．90％，多化平均8．72％。茧层生产效率与茧层量、茧层率、5龄一日茧层量成正相关，达显著或极显著水平；与5龄经过、全龄经过有正相关倾向，但不显著；与虫蛹率、死笼率、全茧量、消化率无一致倾向的相关。还调查了消化率和茧层转换率。     

酸角挥发油香味成分分析及在卷烟中的应用

利用超临界CO2流体萃取酸角挥发油，平均出油率为2．46％。并利用气相色谱仪对酸角挥发油的20种主要香味成分进行了定量分析。通过卷烟加香试验发现，酸角挥发油能有效地掩盖卷烟杂气，使烟气柔和，香气细腻，对改进烟气质量作用明显。     

家蚕品种最适环境片区的划分

对家蚕品种最适环境片区划分进行了研究，初步得到如下结果：参试的8个地区可以划分为两个环境片区。第一个环境片区包括四川、重庆和浙江；第二个环境片区包括陕西、山东、安徽、湖北和镇江；第一个环境片区的最适基因型是品种B；第二个环境片区的最适基因型是品种C。如果在各环境片区推广相应最适基因型比在所有地区只推广品种C，则全区平均万蚕收茧量可提高2．1％。     

Preparation of Cathepsin L1 Monoclonal Antibody Against Fasclala gigantica and Establishment of Double Antibody Sandwich ELISA

【Objective】 This study was intend to obtain cathepsin L1(rFgCat L1) specific monoclonal antibody and construct the double antibody sandwich ELISA.【Method】 Five BALB/c mice were immunized with 1 mg/mL rFgCat L1 protein for four times.Mouse splenocytes were isolated and fused with SP2/0 cells to construct hybridoma cells.Strong positive hybridoma cell lines were screened, 1×10⁶ cells were injected intraperitoneally per mouse to prepare monoclonal antibodies.Antibody titer and antigenic epitope were detected using ELISA method, antibody subtype and specificity were identified using Western blotting method.The double antibody sandwich ELISA was constructed by combining the anti-rFgCat L1 polyclonal antibody, and its sensitivity and specificity were tested.The positive and negative critical value was screened by 20 negative sera with positive control, and the constructed double antibody sandwich ELISA was verified by 47 goat positive sera and 47 dairy cow positive sera.【Result】 After immunization, the antibody titers in serum of 4 mice were all more than 10⁴.After isolated mouse with the highest immune response spleen cells were fused with SP2/0 cells total of 8 of them were positive cell lines were obtained after selective culture.5D5 and 7G6 were identified as strong positive strains with stable antibody secretion.After multiple subcloning screens and subcultures, the antibodies secreted in the cell supernatant were stable, with titers of 2⁹ and 2¹⁰ respectively, with ascites titers of 10⁷ and 10⁸.Western blotting and antibody subtype identification kits identified that the two antibodies were IgG1 type and the light chain was kappa type, both of which could specifically bind FgESP.According to the same antigen site was recognized by the two kinds of antibodies, the antigen titer of the two monoclonal antibodies were comparied, 7G6 was used as the coating antibody, and anti-rFgCat L1 was used as the enzyme-labeled secondary antibody.The optimized condition of method was that 7G6 was coated at a concentration of 2 μg/mL, the dilution concentration of anti-rFgCat L1 polyclonal antibody was 25 μg/mL, the dilution of Don-HRP-conjugated was 1∶4 000, 5% skimmed milk powder was selected as the blocking solution and the color development time was 25 min.The method was proved that could recognize the lowest antigen concentration of 0.625 μg/mL, also could specifically recognize antigen of Fasciola fasciatus.The constructed sandwich ELISA method was used for antigen detection of 47 dairy cow positive serum and 47 goat positive serum infective samples kept in the laboratory and the positive antigen rate were 72.3% and 78.7%, respectively.【Conclusion】 Anti-rFgCat L1 monoclonal antibody was successfully prepared and the double-sheet sandwich ELISA method for fascioliasis was constructed, which provided a good theoretical basis and material basis for the development of low-cost and rapid diagnostic kits.