The Use of Antigen-capture Enzyme-linked Immunosorbent Assay (ELISA) for the Diagnosis of Rinderpest and Peste des Petits Ruminants in Ethiopia

Rinderpest had been reported in most parts of Ethiopia when the Pan African Rinderpest Campaign (PARC) was launched. As a result of intensive disease investigation and strategic vaccination, most parts of the country are now considered provisionally free, and widespread vaccination has been replaced by clinical and serological surveillance. Details of any episodes of disease are recorded and followed up after laboratory confirmation of suspected cass using antigen-capture ELISA. This paper is based on observations on the performance of the antigen detection ELISA compared to the agar gel immunodiffusion (AGID) test, which also differentiates rinderpest from peste des petits ruminants (PPR). The stability of the specific viral antigen was monitored for 4 days, and rinderpest and PPR antigens were still detected, depending on the type of specimen. Antigen capture ELISA is more rapid, sensitive and virus specific than the AGID. Even if the cold chain of the specimen is compromised for a day or two during sample collection and submission, the specimen may still be suitable for testing by ELISA.     

Plasma membrane of trout spermatozoa: I. Isolation and partial characterization

The plasma membrane from spermatozoa of rainbow trout was isolated by four techniques: sonication, hypotonic shock, mechanical homogenization after freeze-thawing, and nitrogen cavitation, in combination with continuous sucrose gradient centrifugation. Nitrogen cavitation (900 PSI, 20 min equilibration at 4°C) was the most effective technique.Following nitrogen cavitation, four bands were recovered in the sucrose gradient at densities 1.03, 1.05, 1.09 and 1.15 g/ml. Electron microscopy revealed membrane vesicles of various sizes in bands 1 to 3, while enzyme analysis revealed a 3.9 to 5.5-fold enrichment in 5'-nucleotidase and little contamination by lactate dehydrogenase (cytosol) and succinic dehydrogenase (mitochondria). Lipid analysis of bands 1 and 2 indicated a 6 to 7-fold enrichment in cholesterol and a cholesterol: phospholipid ratio of 0.59–0.70. Seven classes of phospholipids were present in bands 1–3 with no significant differences observed among bands. These data indicate that the vesicles (in bands 1 and 2) obtained after nitrogen cavitation are primarily plasma membranes. Membranes in band 3 appear to be slightly contaminated with nuclear membranes.Most of the plasma membrane proteins were acidic to neutral. The 2 main membrane proteins were 42 and 30 Kilodaltons.     

苜蓿干草调制过程中群体特征对粗蛋白质含量的通径分析

通径分析表明,苜蓿干草在调制过程中含水量与粗蛋白质之间的相关关系主要是通过中性洗涤纤维(NDF)和叶片损失率的间接影响产生的;NDF与粗蛋白质的相关关系,直接作用和通过叶片损失率的间接作用各占50%,并且通过含水量所起的作用为正效应;叶片损失率与粗蛋白质含量的相关关系,主要是直接作用(占56.0%)和通过NDF的间接作用(占54.5%)所引起的.     

双条杉天牛OBP与其嗅觉相关化学物质的分子对接及分子动力学分析

为探究双条杉天牛Semanotus bifasciatus气味结合蛋白（odorant binding protein,OBP）与信息化合物的识别结合机理,筛选出与OBP结合效果最佳的化学配体,选择在双条杉天牛触角中高表达的OBP2、OBP6、OBP7序列,通过Swiss-model在线软件对其进行同源模建,使用ERRAT程序、Procheck和Verify＿3D进行建模质量评估;采用Autodock软件对OBP模型和相关化学物质进行分子对接分析,通过GROMACS 2019.6软件包进行目的OBP与结合效果最佳配体复合体的分子动力学模拟。结果显示,OBP2、OBP6和OBP7模型所得到的ERRAT值分别为97.03%、94.06%和97.39%,三维结构与一级结构的兼容性所得评分大于0.2的氨基酸百分比均大于80.00%,且拉氏构象图中氨基酸位于最佳合理区的数量均大于90.00%,说明所建模型合理;在11个化合物中,柏木醇与OBP2、OBP6和OBP7的结合性能最好,结合能分别为-7.04、-6.86和-6.62 kJ/mol,在结合过程中,疏水作用力和氢键作用力共同作用使蛋白与配体...     

降解大豆分离蛋白AMPS接枝共聚物的合成与表征

利用酸性蛋白酶对大豆分离蛋白(SPI)进行降解,得到一系列降解大豆分离蛋白(HSPI).以过硫酸铵为引发剂、2-巯基乙醇为蛋白质变性剂,在浓度为8 mol/L尿素溶液中进行HSPI与水性单体2-丙烯酰胺基-2-甲基丙磺酸(AMPS)的接枝共聚,得到接枝产物(HSPI-g-PAMPS).研究了水解度、引发剂用量、反应时间和单体用量对接枝率和接枝效率的影响,并对HSPI和HSPI-g-PAMPS的溶液性能进行了研究.结果表明:HSPI-g-PAMPS的Zeta电位明显降低、在低剪切速率下HSPI-g-PAMPS的粘度最大.     

Cloning of vacuolar H<Superscript>+</Superscript>-ATPase subunit c genes from Japanese iris,and functional characterization in yeast

Five different isoforms (IrlVHA-c1-c5) of V-ATPase subunit c (VHA-c) were cloned from a Japanese iris (Iris lactea Pall. var. chinensis Fisch. Koidz) cDNA library using degenerate primers PCR and the 5'-RACE technique. The sequence analysis showed the open reading frame (ORF) of the IrlVHA-c1 c5 to be 495 bp, corresponding to a protein of 164 amino acids. Among the five isoforms, IrlVHA-c1 and IrlVHA-c2 are completely homologous. The IrlVHA-c protein is localized at the vacuolar membrane as indicated by a g...     

四川不同地区3种丛生竹叶片可溶性蛋白分析

以四川省不同地区慈竹、梁山慈竹及硬头黄竹的叶片为材料,测定叶片可溶性蛋白质含量,采用SDS-PAGE技术研究其可溶性蛋白谱带的差异。结果表明,不同地区、不同竹种叶片可溶性蛋白含量及其谱带均有差异,可溶性蛋白含量以雅安地区的梁山慈竹最高,其次为雅安荥经地区的慈竹,宜宾江安地区的硬头黄竹最低;可溶性蛋白谱带慈竹以邛崃地区的为最多,梁山慈竹以雅安地区的为最多,硬头黄竹以宜宾竹海地区的为最多。     

安徽休宁倭竹竹叶主要营养成分的研究

休宁倭竹(Shibataea hispida McClure)是安徽特有竹种.由于其竿形矮小,竹材、竹笋等利用价值较低,长期以来处于自生自灭状态.调查发现休宁倭竹叶片一直被牛、羊所喜食,经对休宁倭竹新生叶片的营养成分的测定,倭竹叶含水率、粗蛋白质、粗脂肪、粗纤维、总灰分、多糖的含量分别为56.88%、2.2%、4.54%、24.64%、5.99%、0.411%;每100 g竹叶内含维生素C 58.67 mg、Zn 4.901 mg、Cr 1.947mg、Fe 23.237 mg、Cu 2.062 mg.结果表明:与常见的饲用作物比,休宁倭竹叶的粗脂肪、维生素C、Zn、Cr、Fe、Cu的含量比较高,粗纤维含量处于中等水平,灰分和粗蛋白质含量比较低,适宜作青饲料或作为牲畜的主饲料的配料.该研究旨在为休宁倭竹合理的开发、科学的保护提供理论依据.     

香蕉束顶病毒基因组和编码蛋白的研究进展

香蕉束顶病毒是一直径约为18～20nm的等轴病毒粒子，基因组至少由6个组分组成。它们有3个共同区域：开放阅读框架、主要共同区和颈环共同区。这些组分编码的蛋白为：BBTV DNA—1编码了复制起始蛋白；BBTV DNA—2和BBTV DNA—6编码的蛋白功能未定；BBTV DNA—3编码病毒的外壳蛋白；BBTV DNA—4编码运动蛋白；BBTV DNA—5编码类成视网膜细胞瘤结合蛋白。