Fas/FasL系统调控公牛精子发生的研究进展

牛的精子发生是一个特殊的雄性生殖细胞的分化、增殖和凋亡的过程。在这一过程中,生殖细胞总是不断地处于分化和凋亡的动态平衡之中。凋亡信号转导分子Fas和FasL组成的Fas/FasL系统不但在细胞自稳和疾病发生中具有重要作用,而且参与调控牛的精子发生。当Fas和FasL表达异常时,直接影响生殖细胞的发育和增殖。作者通过阐明Fas/FasL系统在牛精子发生过程中的调控机理,为提高种公牛的繁殖性能开创一条新的途径。     

Changes in the expression of Bcl-2 and Fas in HL-60 cells treated with cyclosporine A

AIM: To investigate the changes of the expression of Bcl-2 and Fas protein and the apoptosis in HL-60 cells induced by cyclosporine A. METHODS: The expression of Bcl-2 and Fas protein and apoptosis in HL-60 cells were measured by immunohistochemistry analysis and flow cytometric analysis. RESULTS: There was strong expression of Bcl-2 in HL-60 cells, treatment with cyclosporine A (CsA) for 8-10 h down-regulated the expression of Bcl-2. Fas protein expression in HL-60 cells was very low, CsA induced apoptosis of HL-60 cells, but didn't induce Fas protein expression. CONCLUSION: CsA induces apoptosis in HL-60 cells by down-regulating Bcl-2 expression.     

猪Fas基因的克隆及序列分析

从PK-15细胞中提取总RNA,用RT-PCR方法扩增出猪F as基因,将其克隆到PM D 18-T载体上,进行序列分析。结果表明,克隆的猪F as基因序列与G enB ank上登录的猪F as基因同源性为100%,与人、牛、羊的F as基因核苷酸及推导的氨基酸序列同源性分别为73.4%、79.2%、76.4%和56.2%、67.0%、64.6%。F as蛋白胞内区的死亡域,其氨基酸序列在猪、人、牛和羊的F as基因中呈现较高的同源性。     

Involvement of the Fas and Fas ligand in testicular germ cell apoptosis by zearalenone in rat

Zearalenone (ZEA), a nonsteroidal estrogenic mycotoxin, is known to cause testicular toxicity in animals. In the present study, the effects of ZEA on spermatogenesis and possible mechanisms involved in germ cell injury were examined in rats. Ten-week-old Sprague-Dawley rats were treated with 5 mg/kg i.p. of ZEA and euthanized 3, 6, 12, 24 or 48 h after treatment. Histopathologically, spermatogonia and spermatocytes were found to be affected selectively. They were TUNEL-positive and found to be primarily in spermatogenic stages I-VI tubules from 6 h after dosing, increasing gradually until 12 h and then gradually decreasing. Western blot analysis revealed an increase in Fas and Fas ligand (Fas-L) protein levels in the ZEA-treated rats. However, the estrogen receptor (ER)α expression was not changed during the study. Collectively, our data suggest that acute exposure of ZEA induces apoptosis in germ cells of male rats and that this toxicity of ZEA is partially mediated through modulation of Fas and Fas-L systems, though ERα may not play a significant role.     

Apoptosis in Ichthyophthirius multifiliis is associated with expression of the Fas receptor of theronts

The expression of type I membrane Fas receptors on the surface of Ichthyophthirius multifiliis (Ich) theronts and the possible association between Fas expression and theront apoptosis induced by the immune antibody was examined. Fas receptors were detected on the theront surface using fluorescein isothiocyanate-conjugated mouse monoclonal antibody against Fas. Fas-positive theronts significantly increased with time during in vitro incubation and with increasing theront concentration. Furthermore, the immune cutaneous antibody induced theront apoptosis; however, Fas ligand did not. A highly significant correlation was noted between theront Fas expression and immune cutaneous antibody-induced theront apoptosis. Numbers of apoptotic theronts increased with increasing number of Fas-positive theronts. The data indicated that theront apoptosis induced by immune cutaneous antibody appears to be positively correlated with the expression of Fas on the surface of Ich theronts.     

Effects of Tertram ethylpyrazine on expression of Fas/FasL mRNA in lung tissue with ischemia-reperfusion injury in rabbits

AIM: To study the expression of Fas/FasL mRNA in lung tissue with ischemia-reperfusion lung injury in rabbits and the relationship with the apoptosis,and to observe the effects of Tertram ethylpyrazine on them.METHODS: The pulmonary ischemia-reperfusion models in rabbits with occlusion of left pulmonary hilum for 1 h and then reperfusion 1,3,5 h respectively were used in this experiment.In TMP group,Tertram ethylpyrazine was intravenously dropped at dose of 60 mg/kg at 1 h before ischemia.The TUNEL technique was used to explore apoptotsis of pulmonary cells.In situ hybridization was performed on the rabbit lung tissue to assay the expression of Fas/FasL mRNA.RESULTS: Apoptosis of pulmonary cells was found in both IR group and TMP group.Compared with group IR,the apoptosis index (AI) was decreased obviously in group TMP (P<0.01).There was a significant positive correlation between the expression of Fas/FasL mRNA and the apoptosis of pulmonary cells (r₁=0.900,r₂=0.869,P<0.01).CONCLUSION: The activation of Fas/Fas-L system may contribute significantly to induce pneumocyte apoptosis in pulmonary ischemic injury.Tertram ethylpyrazine inhibits the activation of Fas/FasL system to decrease apoptosis in pulmonary tissue,which may protect the pulmonary tissues in ischemia injury.     

大肠杆菌O111:B4内毒素对大鼠肝脏Fas蛋白表达的影响

为探讨大肠杆菌O111:B4内毒素(ET)对大鼠肝脏Fas蛋白表达的影响,本研究将48只SD大鼠随机分为试验组和对照组,试验组尾静脉注射ET,对照组尾静脉注射等体积无热源生理盐水;两组大鼠分别于注射后3h、4h、8h、12 h各迫杀6只,采集肝脏分别用于Fas蛋白表达的western blot检测和流式细胞术(FCM)检测.结果显示,试验组大鼠Fas蛋白的表达明显高于对照组(p＜0.01),并且呈上升趋势.该结果表明,ET能够有效上调大鼠肝细胞Fas蛋白的表达.     

圆弧青霉菌毒素—青霉酸对小鼠脾脏组织细胞凋亡和Bcl-2、Bax及Fas/FasL基因mRNA表达的影响

为探讨圆弧青霉菌毒素—青霉酸对小鼠脾脏组织的毒性作用,采用TUNEL法和RT-PCR对染毒后脾脏细胞凋亡和Bcl-2、Bax及Fas/FasL等凋亡相关基因mRNA表达的影响进行研究。结果表明,随着青霉酸染毒剂量的增加,脾脏组织中细胞凋亡数量逐渐增多。染青霉酸低剂量组脾脏组织中Bcl-2、Fas和FasL的表达增加,Bax表达下降,高剂量组和中剂量组中Bax、Fas和FasL表达增加,Bcl-2表达下降,说明青霉酸可能通过促使Bax、Fas/FasL表达增加和Bcl-2表达降低来诱导小鼠脾脏细胞的凋亡。     

尖锐湿疣角质形成细胞凋亡与Fas、Bcl-2及PCNA的表达

目的 :检测尖锐湿疣 (CA)角质形成细胞凋亡和Fas、Bcl 2及PCNA的表达 ,探讨它们在CA发病中的作用及关系。方法 :对 5 1例CA上皮和 18例正常上皮分别采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记技术检测细胞凋亡 ,采用免疫组化ABC法检测Fas、Bcl 2及PCNA的表达。结果 :CA上皮凋亡指数与正常上皮无明显差异 (P >0 .0 5 ) ,CA上皮Fas、Bcl 2及PCNA指数显著高于正常上皮 (P <0 .0 1) ,Fas表达与Bcl 2表达呈中度正相关 (r=0 .318,P <0 .0 5 )。结论 :CA上皮细胞凋亡与Fas、Bcl 2及PCNA的表达可能与CA的发病有关。     

新疆卡拉库尔羊Fas基因的克隆及核苷酸序列分析

为了分析新疆卡拉库尔羊的Fas基因,根据普通绵羊的碱基序列和相关文献设计引物,用RT-PCR扩增并测定了卡拉库尔羊Fas基因的cDNA序列。结果表明,Fas基因cDNA序列包含了一个750 bp的开放阅读框,通过GenBank中的Blastn序列比较分析,卡拉库尔羊的Fas基因与普通绵羊的同源性高达99.69%。这为将来制备卡拉库尔羊Fas基因探针及卡拉库尔羊分子免疫学的发展提供了理论依据。