全文获取类型
收费全文 | 539篇 |
免费 | 20篇 |
国内免费 | 20篇 |
专业分类
林业 | 36篇 |
农学 | 14篇 |
基础科学 | 36篇 |
36篇 | |
综合类 | 252篇 |
农作物 | 11篇 |
水产渔业 | 15篇 |
畜牧兽医 | 150篇 |
园艺 | 26篇 |
植物保护 | 3篇 |
出版年
2024年 | 3篇 |
2023年 | 5篇 |
2022年 | 8篇 |
2021年 | 5篇 |
2020年 | 22篇 |
2019年 | 11篇 |
2018年 | 8篇 |
2017年 | 12篇 |
2016年 | 13篇 |
2015年 | 27篇 |
2014年 | 32篇 |
2013年 | 36篇 |
2012年 | 44篇 |
2011年 | 61篇 |
2010年 | 31篇 |
2009年 | 53篇 |
2008年 | 30篇 |
2007年 | 27篇 |
2006年 | 26篇 |
2005年 | 17篇 |
2004年 | 13篇 |
2003年 | 15篇 |
2002年 | 13篇 |
2001年 | 3篇 |
2000年 | 8篇 |
1999年 | 6篇 |
1998年 | 4篇 |
1997年 | 9篇 |
1996年 | 2篇 |
1995年 | 8篇 |
1994年 | 5篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1987年 | 2篇 |
1983年 | 2篇 |
1981年 | 2篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
排序方式: 共有579条查询结果,搜索用时 15 毫秒
31.
论草业科研院所野外基地建设 总被引:2,自引:0,他引:2
论述了草业科研院所野外试验基地的功能,指出了基地建设中存在的问题,并提出了加强野外基地建设的五条对策:加强基地硬件建设,搭建科研项目实施的良好平台;不断提升基地的软件建设,加快试验进程和降低试验费用;大力发展支柱产业,促进基地综合发展;重视基地发展,配备高素质管理人才;改革创新,变项目型基地为经营型基地. 相似文献
32.
AIM: To explore the effects of peroxisome proliferator-activated receptor γ (PPARγ) agonist on calcium-activated neutral proteinase, calpain, expression in the brain of rats with acute experimental autoimmune encephalomyelitis (EAE). METHODS: EAE model was established in rats by inoculating the homogenate contained spinal cord of guinea pig and complete Freunds adjuvant. Respectively, the PPARγ agonist rosiglitazone and non-steroid anti-inflammatory drug (NSAID) ibuprofen were used to treat the EAE rats. Outcome measures (Kohs scale) were applied at baseline and after treatment to assess the improvement of clinical symptoms. Calpain expression levels were detected by RT-PCR and Western blotting. RESULTS: All the groups showed significant improvements of scales scores after treatment with rosiglitazone and ibuprofen. No significant difference of the expression of calpain mRNA was found among EAE group, rosiglitazone and ibuprofen groups (P>0.05), but the expression of calpain reduced markedly in rosiglitazone and ibuprofen groups compared with that in EAE group (P<0.05). CONCLUSION: Rosiglitazone and ibuprofen inhibit the expression of calpain and improve the clinical symptoms of EAE rats. PPARγ agonist plays a neuroprotective role in EAE rats. 相似文献
33.
利用染色体替换法研制出具有标志基因、又可在子代明确区分的二种精子活力相同的材料——临皓普、临皓素.以这两个材料进行二雄交一雌的混精杂交试验,研究不同交配时间、交配次序对受精选择性的影响.结果,第一雄蛾交配25或30分钟的子代中,素蚕和普斑蚕开差很大,交配50或60分钟,两种类型比例接近,x~2检验理论值与实际值相符.交配次序中,属后交雄蛾的类型略多,交配时间和次序的不同,对选择受精的影响较大,本文对上述问题进行了讨论. 相似文献
34.
根据有关机械原理设计出了一种新型的粉状物质定量取料给料装置,该装置结构简单,定量准确、快速,并且可以重复地取料给料。实验证明,该装置稳定可靠,具有一定的实际应用价值和推广价值。 相似文献
35.
用绵羊进行肺炎病毒接种4只山羊3-4个月后,可观察到接毒山羊都出现了发育迟缓和消瘦现象,并有1只山羊山现了明显的临床病症。而未接毒的对照山羊则发育正常。接毒28d后,可以从接毒山羊的外周血单核细胞中分离到病毒。病理剖检发现4只接毒山羊中有1只山羊的多种器官出现了较为严重的病变,组织学检查则可看到典型的间质性肺炎、中度的脑炎和较为严重的脾炎的症变。以上的结果表明OPPV可以感染山羊,并对山羊有较强的 相似文献
36.
《生物技术原理及应用》实验教学改革的探索 总被引:1,自引:0,他引:1
针对现行生物技术实验教学的薄弱之处,对《生物技术原理及应用》实验教学的改革进行了一些探索,提出了改进和加强实验技能培养的具体建议。 相似文献
37.
38.
Our study focuses on predicting the ultimate short-term load carrying capacity of timber-to-timber connections with dowel-type fasteners. The wide range of possible configurations in practice makes the resolution of these values by tests unrealistic. Moreover, different current regulations do not consider some specific failure mechanisms. In many countries, the reduction of resistance involved by this phenomenon is taken into account by considering an effective number of dowels (nef) smaller than the actual number of dowels (n) in the connection. However, these different regulations disagree on the values of nef and on other points (spacing, partial coefficient of security, formulas). These discrepancies in design rules invite the fundamental research on this topic and, therefore, new methods are sought in order to estimate the load carrying capacities of the connections. In the light of these, our approach consists of predicting the load carrying capacity with the neural network numerical tool. The results obtained by this analysis tool are satisfactory, although the model remains complex. Subsequently, we focus on the simplification of this numerical model with classical regression techniques in order to implement it in a design code. 相似文献
39.
Jong-Hyun Moon Hae-Won Jung Hee-Chun Lee Joon-Hyeok Jeon Na-Hyun Kim Jung-Hyang Sur Jeongim Ha Dong-In Jung 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(2):203-211
In the present study, the use of dogs with experimental autoimmune encephalomyelitis (EAE) as a disease model for necrotizing encephalitis (NE) was assessed. Twelve healthy dogs were included in this study. Canine forebrain tissues (8 g), including white and grey matter, were homogenized with 4 mL of phosphate-buffered saline for 5 min in an ice bath. The suspension was emulsified with the same volume of Freund''s complete adjuvant containing 1 mg/mL of killed Mycobacterium tuberculosis H37Ra. Under sedation, each dog was injected subcutaneously with canine brain homogenate at four sites: two in the inguinal and two in the axillary regions. A second injection (booster) was administered to all the dogs using the same procedure 7 days after the first injection. Clinical assessment, magnetic resonance imaging, cerebrospinal fluid analyses, necropsies, and histopathological and immunohistochemical examinations were performed for the dogs with EAE. Out of the 12 animals, seven (58%) developed clinically manifest EAE at various times after immunization. Characteristics of canine EAE models were very similar to canine NE, suggesting that canine EAE can be a disease model for NE in dogs. 相似文献
40.
Wang?Gui-zhongEmail author Li?Shao-jing Zeng?Chao-shu Lin?Shu-jun Kong?Xiang-hui Ai?Chun-xiang Lin?Qiong-wu 《Aquaculture International》2005,13(5):459-468
This is an initial paper in a series of overviews of biological research and aquaculture development of the mud crab, Scylla serrata, in China. Results of experimental ecological studies on mud crabs are reported here. As a result of these experimental studies, results that are important for mud crab culture were also discovered and these include, methods to condition and manage broodstock, determination of ecological conditions that are suitable for embryonic development, and the influence of temperature, salinity, diet and larval density on development and survival of larvae. Results of this work will be useful in establishing a good method for artificial mass culture of larvae. 相似文献