摘花序、打顶对商洛桔梗生长的影响

研究了摘花序、打顶对2年生桔梗的生长性状及根部有效成分的影响,结果表明:摘花序、打顶后的植株生长健壮、干物质积累多、根部产量高、有效成分积累量大,明显优于2种处理方法的单独使用。     

HPLC法测定不同产地桔梗的桔梗皂苷D含量

采用HPLC法测定了不同产地、不同生长年限的桔梗样品中桔梗皂苷D的含量。结果显示:不同产地、不同生长年限的桔梗样品中桔梗皂苷D含量差别较大;桔梗样品中桔梗皂苷D的含量与其生长年限呈正相关;以山东淄博3年生桔梗中的桔梗皂苷D含量最高。     

福州地区洋桔梗引种栽培研究

洋桔梗是一种优秀切花,为了探讨洋桔梗在福州地区的种植可行性,2008年引种了6个洋桔梗,进行夏季栽培技术试验。结果表明:4月定植的这些洋桔梗品种在福州地区生长发育正常,植株高度60.5 cm以上,花蕾数19个以上,品质指标可以达到花卉市场要求。     

Chilling Tolerance, Cold Hardening and Freezing Tolerance of Low-temperature-tolerant Mutants of Dendranthema × grandiflorum (Ramat.) Kitamura

Two low-temperature-tolerant mutants of the Dendranthema × grandiflorum (Ramat.) Kitamura cultivar ‘Puck’ derived from an x-radiated tissue culture system were compared, as vegetatively growing plants, to ‘Puck’ ssfor several physiological properties under low-temperature conditions. So far, the low-temperature tolerance of the mutants has been defined as the capacity to flower earlier under low temperature conditions (8—15°C) the wild type. All three lines were not chilling sensitive in the vegetative state and were able to acclimatize and to develop freezing tolerance. Differences between ‘Puck’ and the mutants were found but could not be related to the low-temperature tolerance of the mutants in the generative phase. Levels of the amino acid proline increased during the hardening process, reaching the highest level in the cultivar ‘Puck’. No evidence could be obtained that proline is causally involved in the cold-hardening process. Freezing-induced membrane injury was found to decrease with cold hardening (0—28 days) but the two mutants did not show a greater capacity to acclimatize and cold harden than the original cultivar ‘Puck’.     

Population means and correlation analyses of growth parameters in lisianthus (Eustoma grandiflorum Shinn.)

Summary The variation in growth characteristics among lisianthus (Eustoma grandiflorum Shinn.) genotypes was investigated. The genetic control of several growth parameters was studied by analysing parental, F1, BC1 and F2 populations. The parameters analysed were stem elongation rate (SE), stem diameter (SD), leaf width (LW), number of nodes (NN), node length (NL) and days to flowering (DF). The genotypes tested exhibited great variation for all parameters. A particularly wide variation was noted for SE, ranging from 3 to 56 mm/week. This variation was partially attributed to a differential sensitivity to rosette formation. There was a marked positive heterosis for SE, SD, LW and NN; these parameters proved to be genetically correlated within segregating populations. DF was slightly negatively correlated with SE, NN and NL. It was concluded that SE and DF were independently controlled.Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel. No. 1196-E, 1993 series.     

‘小黄’菊遗传转化再生体系的建立

以‘小黄’菊茎段、叶盘为外植体 ,选用MS培养基为基本培养基 ,附加激素 6 BA和NAA ,通过研究不同激素浓度组合对外植体愈伤诱导及不定芽分化的影响 ,建立了较好的遗传转化再生体系 .试验结果表明‘小黄’菊茎段、叶盘最适分化培养基分别为MS +6 BA 2mg L +NAA 1mg L和MS +6 BA 1mg L +NAA 0 1mg L ;小苗的最佳生根培养基为 1 2MS +NAA 0 1mg L ,生根率可达 10 0 % ;4 0mg L卡那霉素可以抑制茎段的分化 ,10mg L卡那霉素可以抑制叶片的分化 ,2 0mg L卡那霉素可以抑制分化小苗的生根 .     

桔梗等3种中药材有机氯农药残留研究

采用丙酮水提取体系、二氯甲烷液液分配、浓硫酸磺化净化技术、气相色谱OV－1701毛细管柱内标法对桔梗、牛蒡子和两头尖中有机氯农药残留进行了研究，结果表明：这3种中药材中均有不同程度的有机氯农药残留，但基本上未超过国家标准中人参有机氯农药最大允许残留限量。     

不同浓度KT预处理液对干藏洋桔梗切花保鲜的影响

为分析KT预处理液对洋桔梗(Eustoma russellianum)切花的保鲜效果,采用不同浓度的KT预处理液处理洋桔梗,干藏后检测鲜切花的瓶插寿命、可溶性糖、花青素、SOD、MDA等生理生化指标。结果表明,干藏洋桔梗切花使用KT预处理液处理后SOD活性增强,MDA含量降低,可溶性糖含量升高,可延缓花青素降解和延长切花瓶插寿命,且提高了洋桔梗切花的观赏品质。其中,2%蔗糖+40 mg/L KT+80 mg/L柠檬酸+200 mg/L 8-HQ配方对洋桔梗切花的保鲜效果最佳。     

不同处理对洋桔梗切花的保鲜效应

研究了赤霉素(GA3)预处理结合瓶插液(蔗糖+8-羟基喹啉)使用对洋桔梗(Eustoma grandiflorum)切花的保鲜效应.结果表明,25 mg/L GA3预处理配合30g/L蔗糖+100 mg/L 8-羟基喹啉瓶插液处理能延长洋桔梗切花的瓶插寿命,增加花枝鲜重,改善水分平衡值,延缓可溶性蛋白质含量的下降,还可以提高保护酶过氧化物酶(POD)和超氧化物歧化酶(SOD)的活性.     

国内洋桔梗组培快繁技术的研究进展

通过查阅现有洋桔梗组织培养资料,对洋桔梗组培快繁技术体系的研究进展进行了综述,主要介绍了洋桔梗组织培养中的外植体的选择、初代培养、增殖培养、生根培养及练苗移栽等程序的研究进展.