诠释修订后《兽药GMP检查验收评定标准》（生物制品）

研究了新旧两版《兽药GMP检查验收评定标准》（生物制品）内容,从条款数量、条款内容和结果判定等方面对新标准的主要变化进行了阐述。     

基于兽药电子追溯的兽药大数据平台建设研究

从国家实施兽药产品电子追溯着手,分析了兽药大数据平台建设的必要性,介绍了大数据的关键技术和分析应用,重点阐述了兽药大数据平台的建设原则和技术架构,对兽药大数据平台建设的关键因素进行了思考,以期为兽药行业发展提供数据支撑。     

一个水稻P450基因的cDNA扩增多态性分析

在盐胁迫下,经不同药物处理的水稻R6幼苗,提取RNA并反转录为cDNA后,对水稻P450基因cDNA序列扩增多态性进行分析.结果表明,不同药物处理的水稻R6幼苗形态特征明显;药物环孢素(CsA)处理的水稻细胞色素P450基因CYP709(NM-001066006)缺失430 bp条带,可能是NM-001066006基因与NM-001066009基因的外显子选择性剪接所致,从而表现出功能基因扩增多态性.     

Effect of Jinan injection on ultrastructure of lung cancer cell lines

AIM: To investigate the effects of Chinese medicine, Jinan injection, on ultrastructure and mitochondria in cultured lung cancer cell lines. METHODS: The cultured lung cancer cell lines PG and PAa were used and divided into 4 groups: control (C), cisplatin (DDP), Jinan (JA) and Jinan in combination with cisplatin (DJ), respectively. The changes of morphology and mitochondria membrane potential, intracellular Ca²⁺ and pH in every group were observed by inverted microscope and electronic microscope as well as by using flow cytometry, staining by rhodamine, Fluo-3 and BCECF, respectively. RESULTS: Degeneration cells showed chromatin condensation and peripheral congregation. In cytoplasm autophage lysosome increased and myelinoid body was seen easily. In mitochondria structure, where the space between the inner and outer membranes of these organelles expanded as the matrix was compressed. The electron-dense or swelled was observed as vacuole degeneration and its matrix showed electron-lucent. Compared to control, mitochondria membrane potential increased in every group after 24 h and 48 h treatment. DDP increased intracellular calcium ion in PG cells, however, in PAa cells, JA and DJ decreased it. Intracellular pH got lower at 24 h and higher at 48 h in PG and PAa cells. There were significance in every group vs control in PG and PAa by statistic t-test (P＜0.01). CONCLUSION: Apoptosis was induced in PG and PAa cell lines by Jinan injection and DJ. Mitochondria matrix displayed electron-dense, mitochondrial potential, intracellular calcium ion and pH showed an increasing trend. Mitochondria damages may play an important role in apoptosis induced by Jinan and DJ.     

Effect of zhiganning on the expressions of HSP60 and HSP70 in the hepatocytes of rats undergoing steatohepatitis

AIM: To observe the expressions of HPS60 and HPS70 in hepatocytes in rats under treatment with zhiganning on steatohepatitis. METHODS: Male SD rats were randomly divided into large dose zhiganning group, small dose zhiganning group, ursodeoxycholic acid (UDCA) group, model group, normal control group. Except the normal control group, all the other rats were fed with high fat (88% standard diet, 10% lard, 2% cholesterol) and 35% alcohol 10 mL/kg twice a day. Prophylactic drugs were used at the same time. All rats were sacrificed at the 9th week. Routine histologic features of hepatic sections were observed by HE staining and penetrated electron microscope. The expressions of HSP60 and HSP70 in the liver were detected by immunohistochemistry, respectively. RESULTS: ⑴ The degree of steatohepatitis in the large dose zhiganning group and ursodeoxycholic acid (UDCA) group were significantly decreased compared with that in model group (P<0.05). ⑵ The expression of HSP70 in the large dose zhiganning group and ursodeoxycholic acid (UDCA) group were significantly higher than that in either model group or normal control group (P<0.05, P<0.01, respectively). The expression of HSP60 in the large dose zhiganning group was significantly higher than that in either model group or normal control group (P<0.05, P<0.01, respectively). ⑶ In the large dose zhiganning group and ursodeoxycholic acid group, ultramicroscopic structure of liver was nearly normal, which was significantly improved compared with model group. CONCLUSION: The results indicate that zhiganning and UDCA effectively prevente the steatohepatitis in rats induced by high fat diet and alcohol. The enhanced expressions of HSP60 and HSP70 may play an important role in the prevention of liver from injury.     

辣蓼的应用及其功效

综述了中草药辣蓼在植物源农药、医药保健以及兽药等方面的应用。辣蓼在医药保健中的应用主要表现在它具有抗微生物活性、抗氧化作用以及抗病毒作用中;在植物源农药上主要运用辣蓼具有杀虫、拒食、驱避等活性作用;而在兽药方面主要运用其根或全草治疗多种疾病,均取得了很好的疗效。最后就辣蓼作为植物源农药的独特优越性作了简单扼要的介绍。     

中药提取液对食品中细菌代谢作用的微量热法研究

采用微量热法研究中药提取液对食品中细菌的代谢作用,对于探讨中药提取液的药理药效和对人体的保健作用具有重要意义。本研究采用微量热法测定了不同浓度的银杏叶提取液、芦荟提取液对食品中大肠杆菌代谢作用的热功率——时间曲线,按指数生长模型计算出大肠杆菌在不同浓度的银杏叶提取液、芦荟提取液中的生长速率常数,确定了最适用银杏叶提取液和芦荟提取液的质量浓度。结果表明,银杏叶提取液、芦荟提取液对大肠杆菌具有促进代谢作用。     

金银花优化生产技术规范化操作规程

根据GAP要求和豫金银花的生产实际,参考大量文献资料,集成金银花生态、种质、育苗、栽培、加工等方面的现代成熟技术,优化了金银花生产技术规范化操作规程,以达到安全、有效、稳定、可控的目的.