Effects of three Chinese formulas on BDNF,TrkB in rat contex and hippocampus with chronic immobilization stress

AIM: To investigate the changes of brain-derived neruotrophic factor (BDNF), tyrosine kinase B (TrkB) in rat cortex and hippocampus with chronic immobilization stress and the influence of three Chinese formulas (Xiaoyaosan, Sijunzitang, Jinkuishenqiwan) on them. METHODS: Chronic immobilization stress method (180 min daily, repeated 7 days or 21 days) was taken, and the changes of BDNF, TrkB in rat forehead cortex and hippocampus CA1 were measured by immunohistochemistry integrated image analysis. RESULTS: The contents of BDNF in rat forehead cortex and hippocampus CA1 were obviously lower in the model group of 7 days and 21 days than those in the normal control group (P<0.05, P<0.01), especially the lowest in the model group of 21 days. The contents of TrkB in rat forehead cortex and hippocampus CA1 were higher in the model group of 7 days and 21 days than those in the normal control group (P<0.05, P<0.01). All three Chinese formulas increased the intergral absorbance of BDNF in rat cortex and particle number of BDNF in rat hippocampus CA1. The particle number of TrkB in rat hippocampus CA1 and cortex, and intergral absorbance of TrkB in rat hippocampus CA1 were reduced by Xiaoyaosan. The intergral absorbance of TrkB in rat forehead cortex was reduced by Sijunzitang and Jinkuishenqiwan. The particle number of TrkB in rat forehead cortex was decreased by Jinkuishenqiwan. Compared with the influence of BDNF in response to three Chinese formulas, effect of Xiaoyaosan was more remarkable.CONCLUSION: Decreased BDNF in rat forehead cortex and hippocampus CA1 may participate in changes of chronic immobilization stress, and it can be reversed by the Chinese herbs of soothing liver, strengthening spleen, nourishing kidney, but the effect of Xiaoyaosan is better than that of Sijunzitang and Jinkuishenqiwan.     

鳖病防治中的科学用药

在我国的鳖养殖生产中,用药方面还存在着某些问题。本文根据国内外对鳖用药物的研究、使用状况和我国目前鳖病用药的现状,提出了科学用药的一些见解。     

药品管理系统的设计与实现

本文主要介绍了在Windows 2000下,以JSP Tomcat SQL Server 2000为开发工具开发的药品管理系统。该管理系统主要有药品入库管理、药品库存管理、药品分类管理、厂家信息管理、用户管理、药品查询、药品购买、看药店公告及用户注册系统等几大功能模块。     

Effects of Retinervus luffae fructus on serum lipid level in experimental hyperlipidemia rats

AIM: To investigate effects of Retinervus luffae fructus (RLF) on level of serum lipid and body weight in hyperlipidemia rats. METHODS: Thirty-two male SD rats were randomly divided into four groups: control group (A), hyperlipidemia group (B), hyperlipidemia + RLF group (C), RLF group (D). Both group A and C were fed normal diet every day, while group B and group D fed high fat diet. Meanwhile, group C and D were administered with RLF solution at the dose of 10 mL/kg, respectively for 14 days, while group A and B were administered with drinking water. RESULTS: (1) At the end of experiment, a significant reduction was found in the levels of serum total cholesterol (TC) and triglyceride (TG) of group C animals treated with RLF solution; (2) The levels of serum TC of group D was progressively decreased compared to the level of serum TC at the beginning of experiment; (3) The level of high-density lipoprotein cholesterol (HDL-C) of group C remained unaltered 8d after treatment with RLF solution; (4) The body weight in group C was obviously lower than that in group B. CONCLUSION: RLF had an obvious hypolipidemic effect on hyperlipidemia rats. It can inhibit the decrease in the HDL-C and the increase of body weight in rats.     

Effect of Ershen Zeshu Mixture on the peritoneal lymphatic stomata and the ascites drainage

AIM: To further study the effect of Ershen Zeshu Mixture, a Chinese medicine, on the peritoneal lymphatic stomata and the ascites drainage in experimental mice model of CCl₄-induced fibrosis.By the application of NO supplier (sodium nitroprusside,SNP) and NO suppressor (N^G-mononethyl-L-arginine,L-NMMA),the effect of NO on the peritoneal lymphatic stomata and the ascites drainage was studied to clarify the relationship between Ershen Zeshu Mixture and NO in the regulation of the peritoneal lymphatic stomata.METHODS: The mouse model of fibrosis was established with the application of intragastric installations of carbon tetrachloride one time every three days;Scanning electron microscope and computer image processing were used to detect the girth,area and distribution density of the peritoneal lymphatic stomata;the concentration of urinary ions was measured in the experiment.RESULTS: ①Compared to NS group and model group,Ershen Zeshu Mixture could significantly increase the girth and the area of the peritoneal lymphatic stomata,promote its distribution density (P <0.01) and enhance the drainage of urinary ions such as sodium,potassium and chlorine(P<0.05 or P <0.01).②The effect of Ershen Zeshu Mixture on the peritoneal lymphatic stomata was altered by adding NO supplier or NOS suppressor to the peritoneal cavity,that is,NO supplier can enhance the regulation effect of Ershen Zeshu Mixture on the peritoneal lymphatic stomata,but NO suppressor can antagonize the Ershen Zeshu Mixture's effect.CONCLUSIONS: ①These observations indicated that Ershen Zeshu Mixture might regulate the peritoneal lymphatic stomata to drainage materials in the peritoneal cavity.②The effect of Ershen Zeshu Mixture on lymphatic stomata and ascites drainage maybe have relationship with endogenous NO.     

药物控制释放体系动力学研究进展

针对控制释放系统的不同种类,对近年来药物控制释放体系的动力学研究进行了综述。     

Effects of nuclear factor-kappa B decoy oligodeoxynucleotides on generation of nitric oxide and reactive oxygen species in lipopolysaccharide-induced mouse macrophages

AIM: To study the effects of nuclear factor-kappa B decoy oligodeoxynucleotides on generation of nitric oxide and activated oxygen in lipopolysaccharide-induced mouse macrophages.METHODS: Mouse macrophage cell lines J774.1 cells were cultured with lipopolysaccharide and liposome-mediated oligodeoxynucleotides, and the level of nitric oxide, activated oxygen and inducible nitric oxide synthase were measured. RESULTS: Nuclear factor-kappa B "decoy"oligodeoxynucleotides decreased the activity of inducible nitric oxide synthase in lipopolysaccharide-induced macrophage, inhibited generation of nitric oxide and reactive oxygen species. The level of nitric oxide, activated oxygen and inducible nitric oxide synthase did not change in control group.CONCLUSION: Nuclear factor-kappa B "decoy"oligodeoxynucleotides decrease the generation of nitric oxide and activated oxygen in lipopolysaccharide-induced macrophages, which is probably due to inhibiting the binding sites of activated nuclear factor-kappa B specially.     

Effects of thymopeptide and 4 polysaccharides isolated from Chinese medicine on immune functions of spleen and tumor tissues in U14 cervical cancer-bearing mice

AIM: To identify the enhancement of relatively specific immune responses by thymopeptide and 4 polysaccharides isolated from traditional Chinese medicine in the mice bearing cervical cancer. METHODS: The model of cervical cancer-bearing mice was established by implantation of cervical cancer U14 cells into the armpit of the right forelimb of the mice. Beginning from the 2nd day of tumor implantation, 4 polysaccharides Lycium barbarum polysaccharide (LBP), Angelica sinensis polysaccharide (ASP), Ganodema lucidum polysaccharide (GLP) and Panax ginseng polysaccharide (PGP)], thymopeptide or saline solution were intragastric administered for 21 days according to the experimental design. The animals were then killed, the tumor inhibitory rate and spleen index in treatment groups were compared with those in control group. The splenic T-lymphocytes (TLC) and natural killer (NK) cells, and the content of IFN-γ and IL-10 in the tumor tissues were also determined by flow cytometry and immunohistochemical methods. RESULTS: The order of tumor inhibitory rates was: ASP < PGP < thymopeptide < LBP < GLP. The effects of GLP, LBP and thymopeptide were statistically different from that of control group. No difference of tumor inhibition between PGP/ASP groups and control group was observed. The order of spleen index was: ASP < tumor control group < thymopeptide < GLP < PGP < LBP, that of CD4/CD8 enhancement was: PGP < ASP < GLP < thymopeptide < LBP, and that of CD49b⁺ NK hyperplasia was: ASP < thymopeptide < LBP < GLP < PGP,with statistically significant differences among the groups. The changes of IFN-γ and IL-10 in the tumor tissues showed that Th1/Th2 in thymopeptide group, LBP group and GLP group shifted to Th1. No shift to Th1 in PGP group and ASP group was observed. CONCLUSION: LBP, GLP and thymopeptide show significant inhibitory effects on tumor growth in U14 cervical tumor-bearing mice and can enhance the immune functions in the animals.     

NO isn’t involved in the effect of TMP on vasomotion of arterioles in the rabbit mesentery

AIM: To elucidate the role of NO in the effect of tetramethylpyrazine (TMP) on vasomotion of arterioles induced by noradrenalin(NA).METHODS: Albino rabbits were anesthetized,respiration were maintained,the arterial pressure were measured with a pressure transducer attached to a polyethylene canula in the left common carotid artery,the intestinal loop was mounted on the stage of an inverted microscope and bathed in balanced solution.The inside diameter of the arterial was manually measured on a TV monitor using the TV camera mounted on the microscope.RESULTS: Topical applicated NA to measured field inducd spontaneous vasomotion which has frequency and amplitude.TMP inhibited vasomotion in vasodilation. Treatment with L-NMMA did not affect the effect of TMP.CONCLUSION: L-NMMA can not inhibit action of TMP on vasomotion,TMP may act on smooth muscle directly, not through endogenous NO.     

Effect of Fu-Sheng powder on ischemia-reperfusion-like injury in neural cells

AIM: To further elucidate the mechanism underlying the protective effect of Chinese herb Fu Sheng powder on vascular dementia. METHODS: Primary passage of neural cells of new born rats were subjected to the ischemia-reperfusion-like injury of hypoxia plus glucose deprivation followed by reoxygenation plus glucose, and the effect of "Fu-Sheng powder" on neural cells was examined. RESULTS: Both 5 hours of "ischemia" and 5 hours of "ischemia" plus 5 hours of "reperfusion" led to severe injury to neural cells, the formation of MDA and intracellular calcium concentration increased significantly, however, the activities of SOD and fluidity of neurons decreased significantly. It was also observed that Fu-Sheng powder could significantly alleviate this injury. CONCLUSION: Fu-Sheng powder had direct protective effect on neurons subjected to iscehmia-reperfusion-likeinjury, which might be one of the mechanisms underlying its therapeutic effect on vascular dementia.