"十二五"期间凤城市5种主要林业有害生物灾害损失评估

根据凤城市"十二五"期间林业有害生物发生情况,筛选出美国白蛾、日本蚣干蚧、栗山天牛、杨干象、杨树烂皮病5种主要林业有害生物作为灾害损失评估对象,进行直接和间接经济损失评估计算。结果表明,凤城地区"十二五"期间5种主要林业有害生物造成的年均损失为61 376.777万元,其中,直接经济损失87.218万元,间接经济损失为61 289.559万元。     

Hepatic AA amyloidosis in a cat: cytologic and histologic identification of AA amyloid in macrophages

A 3‐year‐old, spayed female, Domestic Shorthair cat presented with anorexia, lethargy, vomiting, probable hemoabdomen, and multiple masses on the right lateral liver lobe. Clinicopathologic and imaging abnormalities included anemia, azotemia, icterus, and hepatomegaly with hypoechoic masses. On cytologic evaluation of a fine‐needle aspiration of a liver mass there was abundant extracellular pink‐ to purple‐colored material between hepatocytes. The amorphous material was stained with direct fast scarlet (DFS), and green birefringent areas were observed under polarized light, confirming the presence of amyloid. A unique finding on the cytologic smear were macrophages containing amorphous and fibrillar amyloid‐like protein. Histopathologic examination using H&E and Congo red staining confirmed amyloid deposits within the space of Disse, along the sinusoids, portal tracts, blood vessel walls, and within the cytoplasm of macrophages. Immunohistochemical staining with anti‐AA amyloid antibodies further confirmed the presence of AA amyloid. To the author's knowledge, this is the first report of the cytologic finding of AA amyloid protein within macrophages and DFS stain detection of amyloid on a cytologic smear.     

茉莉酸及其甲酯与植物抗虫作用

综述了茉莉酸及茉莉酸甲酯在植物直接抗虫和间接抗虫中的作用。     

基于株型的水稻冠层光合生产模拟

利用基于冠层光分布的光合作用模型,以紧凑型和松散型2个不同株型水稻冠层为对象,通过设计不同的模型输入参数组合,定量分析了不同株型水稻冠层直接辐射消光系数的日变化特征、正午时刻直接辐射在冠层内的垂直分布特征、冠层内光合作用速率的垂直分布特征、不同辐射强度下冠层光合作用速率随叶面积指数的变化特征及其日变化等。结果表明,紧凑型的增产潜力依赖于较大的叶面积指数、叶片光合效能、太阳高度角和太阳辐射强度等。研究结果为水稻高产栽培及理想株型的优化设计提供了支撑。     

棉花穴盘育苗移栽高产示范试验

采用对比设计,研究了棉花穴盘育苗移栽栽培方式与地膜直播栽培方式（CK）对棉花生长和产量的影响。结果表明：棉花穴盘育苗移栽栽培方式的棉花生育期较CK长12 d,虽然全生育期整齐度和苗期生长势较CK差,但花期和絮期的生长势好于CK,单株成铃数明显增多,增产显著。棉花穴盘育苗移栽栽培方式可以在生产上大面积推广应用。     

A Review on Regeneration in Cowpea （Vigna unguiculata L. Walp）

Vigna unguiculata L. Walp is a recalcitrant plant in terms of in vitro cell, tissue and organ differentiation, which makes it difficult to apply tissue-culture dependant approaches for obtaining stable genetic transformation in cowpea. Despite this, sporadic efforts have been made to develop regeneration systems in cowpea during the past 40 years. This review presents the considerable progress on cowpea regeneration （organogenesis and embryogenesis） and especially focuses on the regeneration mode of organogenesis, including highlights of the effect of genotypes, explants, medium and plant hormones used in tissue culture. The existing problems and the future research directions were also discussed.     

植物基因的遗传转化方法

对现有主要植物基因的遗传转化方法进行分析,拟为植物转基因方法的选择提供借鉴。     

浅析油稻轮作双免栽培模式下直播稻田杂草群落演替及综合治理策略

随着油稻轮作双免栽培模式的长期推广与应用,稻田耕作制度、栽培方式、水分管理、田间温室效应发生了相应的变化,稻田杂草害日益突出,草相更加复杂,稻田杂草的优势种群和群落演替较快,现已成为免耕直播稻生产上的一大灾害。针对油稻轮作双免栽培模式下直播稻田杂草种群的变化特点、发生演替的原因及杂草发生的态势作了具体分析,并提出了合理的治理策略。     

Effect of Planting Method on Grain Quality and Nutrient Utilization for No-Tillage Rice

Field experiments were conducted over 2 years using six rice (Oryza Sativa L.) cultivars to investigate the effect of two planting methods, transplanting (TP) and direct sowing (DS), on grain quality and nutrient utilization. Brown and head rice percentages, grain width, amylase, and protein content were greater in TP than in DS, whereas the opposite effect was found for chalkiness rate, gel consistency, and alkali spread value (ASV). Dramatic differences were also found between the two planting methods for nutrient harvest index and utilization efficiency, with TP having greater phosphorus (P) and potassium (K) harvest indexes, nitrogen (N) and K utilization efficiencies, and lower N harvest index and P utilization efficiency than DS. Moreover, significant differences between these genotypes could be found for both nutrient harvest index and utilization efficiency. The correlation analysis showed that rice chalkiness, transparency, gel consistency, amylose, and protein contents were all significantly correlated with N, P, and K nutrient traits. In addition, significant interactions between rice cultivar and planting methods for both rice quality and nutrient traits were also found.     

PCR-ELISA法对大豆品种的转基因定性检测研究

以共价交联在PCR管壁上的寡核苷酸作为固相引物进行PCR扩增,对PCR扩增的固相和液相产物分别进行杂交和凝胶电泳检测的PCR-ELISA法,对黑龙江省常规选育品种合丰35和黑农37、外源DNA直接导入大豆的分子育种所获品种黑生101、及大连进口的美国2号等大豆,进行转基因定性检测.结果表明:该方法高效可靠,可作为一种快速定性检测转基因产品的方法;检测结果:美国2号为转基因大豆,黑生101、合丰35和黑农37为非转基因大豆.上述结果对分子育种、生态保护、安全监测及对建立黑龙江省非转基因大豆生产保护区等具有重要意义.