Fat feeding increases equine heparin-released lipoprotein lipase activity

The aim of this study was to establish the dose-response relationship between fat intake and heparin-released plasma lipoprotein lipase (LPL) activity in horses. Eight mature trotters were fed 4 rations with different fat levels (3.0, 5.0, 7.7, or 10.8% fat in the dry matter) according to a 4 X 4 Latin square design. The experimental rations consisted of hay and different concentrates; the concentrates and hay were given in a 3:1 ratio on an energy basis. Soybean oil was added to the concentrates at the expense of isoenergetic amounts of glucose. Blood samples were taken at the end of each dietary period, which lasted 3 weeks. Fat feeding was found to increase heparin-released plasma LPL activity in a dose-dependent fashion. When the data from this study and previous studies were combined it was calculated that an increase in fat intake by 1 g/kg dry matter is associated with an increase in LPL activity by 0.98 micromol fatty acid released-mL(-1) x h(-1). Fat feeding raised the plasma concentrations of total cholesterol, high-density lipoprotein cholesterol, and phospholipids. Diet did not have a statistically significant effect on plasma triacylglycerol concentrations. The results are discussed in the light of the possible enhancing effect of fat feeding on the oxidative capacity of skeletal muscle.     

日粮类型对猪肉营养和品质的影响

在日粮粗蛋白、能量、钙、磷水平相近的情况下，蛋白饲料种类及微量元素Ｆｅ、Ｃｕ添加水平不同，所饲喂猪只肉质与肉食营养不相一致。根据本试验结果，“豆饼＋鱼粉”型日粮在高剂量Ｆｅ、Ｃｕ的协同作用下有利于促进肌肉的生长和饲料氨基酸向肌肉氨基酸的转化，而“棉籽饼＋菜籽饼”型日粮可期望获得较好的肉质。肌肉Ｆｅ、Ｃｕ、Ｚｎ、Ｍｎ沉积受饲料影响不明显。     

Effect of dietary moisture and sodium content on urine composition and calcium oxalate relative supersaturation in healthy miniature schnauzers and labrador retrievers

The aim of this series of studies was to evaluate two possible feeding strategies as methods for reducing the risk of calcium oxalate (CaOx) formation in two breeds of healthy dog. The studies compared the effect of dietary moisture (Study 1) and dietary sodium (Na), (Study 2) on urine composition of labrador retrievers (LR) and miniature schnauzers (MS). A nutritionally complete dry dog food was fed to 16 dogs (eight LR, eight MS; Study 1) and 15 dogs (seven LR, eight MS; Study 2) for 24 days (Study 1), or 36 days (Study 2). The dogs were fed the diet alone (7% moisture, 0.06 g Na/100 kcal), or supplemented with deionised water to 73% moisture (Study 1), or dietary Na, to deliver 0.20 or 0.30 g Na per 100 kcal (Study 2). Urine pH, volume, specific gravity, and concentrations of 12 analytes were measured for each dog. Urinary relative supersaturations (RSS) with CaOx were calculated from these values. The effects of supplemental Na or water were established using t tests (Study 1) or analysis of variance, and multiple range tests (least significant difference) (Study 2); P<0.05 was considered significant. Increasing dietary moisture significantly increased total moisture intake (P=0.001), and reduced urine specific gravity (P=0.003), urinary oxalate concentration (P=0.04), and CaOx relative supersaturation (P=0.04) in the MS. Urinary parameters remained unchanged in the LR, indicating that feeding a high moisture diet may reduce the risk of CaOx formation in high-risk breeds. Increasing dietary Na led to production of urine with a significantly lower CaOx RSS in both breeds, indicating that sodium supplementation to dry diet formats may reduce the risk of CaOx formation. These feeding strategies should be considered when evaluating methods for preventing CaOx formation within high-risk groups.     

驱除瘤胃真菌对绵羊瘤胃内纤维物质降解的影响

选取3只体况良好,带有永久性瘤胃瘘管的蒙古羊与小尾寒羊的杂交一代公羊,运用尼龙袋法研究全部驱除瘤胃真菌对绵羊瘤胃内稻草纤维物质降解的影响.结果表明：全部驱除瘤胃厌氧真菌后,改变了瘤胃内纤维物质的降解动力学参数,显著降低了瘤胃内稻草DM、NDF、ADF的降解速率（P＜0.05）,显著提高了三者的消化延滞时间（P＜0.05）,显著降低了稻草NDF、ADF的降解率,而DM的降解率有所降低,但差异不显著（P＞0.05）.说明瘤胃真菌在纤维物质降解中发挥了重要的作用.     

笼养蛋鸭日粮电解质和蛋白质水平对体内酸碱平衡的影响

本试验旨在研究日粮电解质和蛋白质水平对家禽体内酸碱平衡的影响。选取5周龄金定鸭240只,随机分为10个处理,每个处理6个重复,每个重复4只,分别饲喂相同代谢能水平(11.7 MJ/kg),不同粗蛋白质水平(16%或19%)和电解质水平(-50、150、250、350和500 mmol/kg)的10种饲粮。血液生化分析试验结果显示:日粮蛋白质和电解质水平互作对pH、CO2浓度、渗透压、HCO-3浓度的影响极显著(P<0.01),对阴离子间隙的影响不显著(P>0.05)。CP为16%,日粮电解质平衡(dietary electrolyte balance,DEB)为-50 mmol/kg组血液pH显著低于其他各组(P<0.05),其他组间无显著差异。CO2、HCO-3浓度和渗透压各组无显著差异(P>0.05)。日粮蛋白质和电解质水平互作对钠、钾、氯离子和肌酐的浓度有极显著影响(P<0.01),对钙、磷、镁离子、血清尿素氮、葡萄糖、甘油三酯和总胆固醇含量的影响不显著(P>0.05)。代谢试验结果显示:粗蛋白质的利用率各组差异显著(P<0.05),粗蛋白质为16%、DEB为500 mmol/kg组蛋白质利用率最高;粗蛋白质为19%、DEB为250 mmol/kg组最低。钙、磷、钾、铜和锰的利用率粗蛋白质为16%、DEB为500 mmol/kg组最高。两因素方差分析结果表明:蛋白质为19%组粗蛋白质及各种矿物元素的利用率都低于粗蛋白质为16%组。日粮电解质和蛋白质水平对家禽体内酸碱平衡有显著交互作用。     

Dietary fibre and indigestible protein increase ileal glycoprotein output without impacting colonic crypt goblet cells in weaned piglets

Mucus plays an important role in gut health by favouring colonisation resistance. The aim of this study was to quantify the impact of fibre and protein on mucin recovery in ileal digesta and on goblet cell histochemistry in the proximal colon. A control diet with highly digestible protein and low fibre and three complex diets containing indigestible protein associated with low, soluble or insoluble fibre sources were tested for 14 days in piglets weaned at 28 days of age. Mucin concentration was determined by ethanol precipitation. Goblet cell subtypes in colonic crypts were analysed by histochemistry. The ileal mucin output was higher with the complex diets than with the control diet (31.6 on average vs 21.7 g/kg DM intake). Increases observed with the low and soluble fibre diets were similar (34 g/kg DM intake). This suggests a limited effect of soluble fibre in presence of indigestible protein. Surprisingly, the observed increase was lower with insoluble fibre (27.2 g/kg DM intake). DM and N output and digestibility were not affected by the diets, but a linear relationship was found between DM and mucin output. In proximal colon, crypt depth, goblet cell numbers per crypt and glycosylation subtypes were not affected by the diet suggesting no change in the capacity of mucus to protect the gut. To conclude, introducing highly indigestible protein in the diet increased ileal mucin output, without effects of these factors on crypt goblet cell patterns in the proximal colon. Introducing fibre in a diet containing highly indigestible protein had a marginal effect.     

Nitrogen balance in barrows fed low-protein diets supplemented with essential amino acids

This study was conducted to determine the effect of addition of essential amino acids (EAA) to low-protein diets on N balance in barrows. Thirty barrows (Duroc × Yorkshire) with an initial BW of 36 kg were fed 5 corn- and soybean meal-based diets containing 13.6 to 18.2% crude protein (CP) for 10 d in a randomized complete block design. The 18.2% CP diet was formulated without addition of EAA (Lys, Met or Thr). The other diets contained 13.6 to 16.5% CP and were supplemented with EAA to provide 0.83% true digestible lysine equal to that in the 18.2% CP diet. Fecal, urinary, and total N excretion as well as N retention (g/d) decreased with decreasing dietary CP level (P < 0.01). The apparent digestibility of N was almost the same for the 18.2 and 16.5 CP diets, but the value was 2% higher (P = 0.07) than that for the 13.6% CP diet. These findings indicate that, compared with the 18.2% CP diets, supplementation with three EAA to the lowest-protein diets (13.6 CP) is inadequate for maximum intestinal protein digestion, amino acid absorption, or tissue protein deposition in growing barrows.     

光照对棉花胚珠纤维离体发育的影响

分析了光照和黑暗条件下，对培养棉花胚珠纤维离体发育的影响。表明在其它条件相同时，纤维离体发育在基因型之间有很大的差异，光照对纤维的发育起了明显的抑制作用。无论受精胚珠还是未受精胚珠，在光照条件下其胚珠成活率、纤维诱导率、纤维生长量、胚珠鲜重均低于在黑暗条件。尤其是未受精胚珠，在光照条件下珂３１２ 与ＴＭ－ １虽能诱导产生纤维，但纤维诱导率很低，仅有８．１９％ 和４．９６％ ，纤维生长量为０．１６３７和０．１１３２，而浙５０６、罗甸铁子和徐州１４２无絮突变体均不能产生纤维。本试验表明：只有在黑暗条件下才适合纤维离体发育。     

预氧丝原位炭化无粘结剂C/C复合材料氧化动力学研究

采用恒温热重法测定了在空气中，０．１ＭＰａ，６７３Ｋ到９７３Ｋ下预氧丝（即预氧化聚丙烯腈纤维）原位炭化无粘结剂Ｃ／Ｃ复合的氧化失重率；并利用扫描电镜分析了这种复合材料氧化前后的结构，氧化动力学测定结果表明：该材料在氧化开始时，其氧化速率随时间的增长而增大，然后随时间的延长而减小，并趋向恒定，研究表明：该材料中预氧丝与基体炭界面结合处是空气中氧的扩散通道，杂质为空气中氧的吸附起到了有附活性中心的催化