两种梭梭出苗对生境土壤基质互换与沙埋深度的响应

以梭梭（Haloxylon ammodendron）、白梭梭（Haloxylon persicum）两种植物为研究对象,采用生境土壤基质互换种植和盆栽控制沙埋深度方法,对其萌发出苗和幼苗建成进行对比研究。结果表明：丘间低地土壤pH、电导率和养分含量均显著高于沙丘顶部,其中电导率（可溶盐含量）相差达5倍;土壤含水量差别不...     

仙客来无土栽培营养液氮磷钾最佳组合和适宜浓度的研究

采用三因素二次D-饱和最优设计方法,对仙客来无土栽培营养液中氮磷钾元素进行最佳浓度组合和适宜浓度范围的试验。结果表明:营养生长期氮磷钾最佳浓度组合为300mg·L-1、60mg·L-1、325mg·L-1,其叶面积高达1924·75cm2,适宜的浓度使用范围分别为263·4～300mg·L-1、20～60mg·L-1、250～325mg·L-1。生殖生长期氮磷钾最佳浓度组合为175mg·L-1、60mg·L-1、400mg·L-1,初花期一次性开放花朵数达34朵,氮磷钾适宜的浓度范围分别为50～175mg·L-1、20～60mg·L-1、100～400mg·L-1。     

复合激素促进仙客来和龙爪菊开花的研究

使用含赤霉素、细胞分裂素、生长素的复合激素处理仙客来、龙爪菊,可促进花芽发育、提高开花率、调节花期。喷施GA150mg/L+NAA20mg/L+V10mg/L能显著提高仙客来花朵数量及整齐度;GA40mg/L+6-BA10mg/L+V5mg/L能促进龙爪菊花瓣、花径增长及整体开放形态改善,延长花期10d左右。     

仙客来分子育种研究进展

在花色、抗病、花型、香味等方面对仙客来分子育种进展及其育种的主要技术策略进行了综述,为仙客来分子育种提供了参考。     

仙客来夏季休眠期叶片光合速率和植株氮磷钾质量分数的变化

仙客来Cyclamen persicum在夏季高温强光的休眠期间蒸腾速率上升,造成水分代谢失调,气孔阻力上升,光照强度和温度上升,光合速率下降.同时整个植株的全氮质量分数缓慢增加,全磷质量分数逐渐下降,全钾质量分数先增加后下降,源叶和根系中氮磷钾质量分数基本呈下降趋势,而球茎中的氮和钾有所积累,磷质量分数基本不变.这些变化趋势与仙客来遇高温强光停止生长而进入休眠的表现相一致.表4参17     

农杆菌介导的黄瓜开花整合子CFL基因转化仙客来

以农杆菌介导法将黄瓜开花整合子CFL基因分别对仙客来幼嫩叶片、叶柄及愈伤组织进行遗传转化,比较了三种转化受体的遗传转化效率.应用超声波辅助的农杆菌介导法,对以叶片为转化受体的仙客来遗传转化体系进行了优化.结果表明:幼嫩叶片是最佳的转化受体,再生植株GUS阳性率达27.25％.叶片在农杆菌浸染时辅以60 s的超声波处理,...     

仙客来标准化栽培技术

通过在内蒙古呼和浩特市地区室内和室外的栽培试验,研究总结出了适应本地区栽值仙客来的湿度、温度,水肥条件,定植、光照、土质要求以及病虫害等其它相关技术要求。为培养高质量、多品种的仙客来提供了基础资料。     

仙客来体细胞胚发生和发育过程中淀粉粒的动态变化

以仙客来(Cyclamen persicum Mill.)开花植株的新生叶片为外植体,诱导筛选胚性愈伤组织,并使其进一步发育成体细胞胚.组织切片观察结果表明:胚性愈伤组织由胚性和非胚性细胞组成,胚性细胞多以胚性细胞团的形式存在,胚性细胞团起源于诱导的胚性决定细胞.体细胞胚起源于单个胚性细胞,经多细胞原胚、球形胚、心形胚和鱼雷胚等时期发育成完整植株.在体细胞胚的发生发育过程中,淀粉粒出现4次积累高峰,分别为胚性细胞、球形胚、早期鱼雷胚和成熟胚发育成完整的小植株时期,淀粉代谢与体细胞胚发生、发育及小植株的形态建成密切相关.     

真盐生植物梭梭和囊果碱蓬幼苗耐干旱能力的研究

本文以沙生植物白梭梭幼苗为对照,研究了盐生植物梭梭和囊果碱蓬幼苗的耐干旱脱水能力,通过比较干旱时间对三者幼苗干旱后存活率的影响,评价其耐脱水能力,并对复水后根部细胞的受损情况进行了研究。结果表明:根长为 12 cm白梭梭幼苗经过7,14,21和28 d干旱后,存活率没有显著性差异,但是梭梭和囊果碱蓬随着干旱时间的延长,存活率发生显著性地降低,囊果碱蓬幼苗干旱28 d后全部死亡并发生降解;白梭梭幼苗的耐干旱能力比较强,干旱28 d后复水根部死亡细胞和受伤的细胞比较少,而梭梭死亡和受损程度明显比白梭梭高;300 mmol /L NaCl溶液处理,梭梭的根尖发生弯曲,根尖大部分死亡。研究表明,白梭梭、梭梭和囊果碱蓬属于“同型叶绿素型”植物,在光照下干旱叶绿体不会被降解,其幼苗在干旱和复水过程中能够适应干旱环境。     

Comparative Analysis of the Role of Substrate Specificity in Biological Control of Botrytis Elliptica in Lily and B. Cinerea in Cyclamen with Ulocladium Atrum

Biological control of Botrytis spp. by the fungal antagonist Ulocladium atrum is based on their interaction in plant tissue. U. atrum is effective against B. cinerea in commercial cyclamen crops but not effective against B. elliptica in lily crops. Based on the necrotrophic nature of the Botrytis spp. and the saprophytic nature of U. atrum it is hypothesised, and experimentally confirmed, that the interaction between Botrytis spp. and U. atrum, resulting in a biocontrol effect, only takes place in necrotic plant tissue. The role of necrotic tissue in the epidemiology of B. cinerea in cyclamen and B. elliptica in lily was found to be different. Removal of symptomless senescing leaves resulted in a significant reduction of the area under the disease severity progress curve (AUDPC) for B. cinerea in cyclamen but had no effect on the disease severity in lily. U. atrum applications significantly reduced B. cinerea AUDPC values in cyclamen but were less efficient than the removal of senescing leaves. In lily, disease severity was not affected by applications of U. atrum. It is concluded that necrotic cyclamen tissue, not killed by B. cinerea, plays an important role in the onset of disease. Colonisation of this tissue by U. atrum prevents saprophytic colonisation of those leaves by B. cinerea. In contrast, conidia of B. elliptica directly infect healthy lily leaf tissue. U. atrum applications aimed at blocking the infection pathway from a saprophytic base are therefore not effective against B. elliptica. Control options based on competitive interactions in and around B. elliptica lesions resulted in a reduced production of conidia by B. elliptica but proved ineffective against disease development. The potential of U. atrum as a biocontrol agent against Botrytis spp. and possibly against other necrotrophs appears to be determined by the competitive saprophytic ability of the antagonist in mutual substrates of pathogen and antagonist and by the role of these substrates in disease epidemiology.