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51.
铁和维生素A及互作对肉仔鸡生产性能和钙磷表观存留率的影响 总被引:2,自引:0,他引:2
选用504只一日龄艾维因肉仔鸡为研究对象,采用完全随机试验设计,随机分成9组,每组4个重复,试验分成0~4周龄和5~7周龄两个阶段。日粮中维生素A有3个添加水平,分别是750、1500、2700I U/kg,铁的添加水平为0、30、60 mg/kg,。结果表明,常规日粮中添加铁对内仔鸡的生产性能影响不显著(P>0.05),添加vA对前后期体增重及日采食量影响均不显著(P>0.05),但对料重比影响接近显著(P≈0.05),Fe和vA交互作用对体增重影响显著(P<0.05)。铁添加水平对钙磷表观存留率影响均不显著(P>0.05),维生素A在前期显著影响钙磷表观存留率(P<0.05),后期仅二者的互作效应对钙磷表观存留率影响显著(P<0.05)。 相似文献
52.
以氯化钙为主要原料,添加磷酸钙和羟乙基纤维素,制备了钙磷胶体复合剂,经检测含钙量为115±5mg/mL、含磷量为16.4±2mg/mL.试验小鼠n=20以0.8mL/20g(体重)的最大剂量一日灌服2次,7d后观察无中毒表现,剖检后组织器管无肉眼可见异常.试验组小鼠n=20以0.4mL/20g(体重)的剂量一日2次连续灌服7d后,无中毒及死亡现象,小鼠平均体重由20.24±1.211g增加到23.35±1.130g,体重增加情况与对照组小鼠无差异.以100mL/只灌服奶山羊(n=5)后,血钙、血磷浓度在2~3h达到峰值(P<0.05),而且可维持6h左右. 相似文献
53.
外源钙处理对马铃薯块茎重量和数量的影响 总被引:2,自引:1,他引:1
以马铃薯品种费乌瑞它(Favorita)为试材,采用营养液无土栽培法,设5个外源钙浓度水平(25,75,150,300和500 mg/L),研究外源钙浓度对马铃薯单株块茎平均重量、数量和块茎内钙浓度的影响。结果表明,随外源钙浓度的升高,马铃薯单株块茎的数量减少。单株块茎的平均重量,以钙水平150,300和500 mg/L增幅较大,显著高于钙水平为25和75 mg/L的处理。单株产量以钙水平25、75、150和300 mg/L的处理显著高于钙水平500 mg/L处理。然而随外源钙浓度的升高,马铃薯商品率明显提高,块茎内钙浓度也相应升高,但块茎内钙浓度与块茎数量呈负相关关系。 相似文献
54.
AIM: The research was to investigate the effects of the Tian ma gou teng decoction on the electric physiology feature of L-type calcium channels in the vascular smooth muscle cells in spontaneously hypertensive rats (SHR), and to further explain the mechanism of the Tian ma gou teng decoction in the intervention of blood pressure.METHODS: 12-week-old SHRs were assigned randomly into five groups:group A (treated with Tian ma gou teng decoction), group B (treated with Tian ma gou teng decoction with subtraction concha haliotidis), group C (treated with nifedipine), group D (treated with concha haliotidis), group E (treated with normal saline as control), each group consisted of 9 rats. After treatments were conducted for 4 weeks, the free calcium concentration in serum was measured. The electric physiology feature of L-type calcium channels in the vascular smooth muscle cells was analyzed by patch clamp technique (PCT).RESULTS: No significant difference between group A and group C was observed in the serum free calcium concentration (P>0.05). There were significant differences among group B, group D and group E (P<0.05), compared to before treatment, the change in group E was the most obvious. A decrease in the L-type calcium channel current of vascular smooth muscle cells was observed in group A and group C. The function of group D was feeble, no decrease in the L-type calcium channels current of vascular smooth muscle cells was observed in group B and group E.CONCLUSION: Tian ma gou teng decoction can increase the serum free calcium concentration and block the L-type calcium channel current in vascular smooth muscle cells, indicating one of the mechanism of intervention of blood pressure. 相似文献
55.
针对甘肃庆阳市某舍饲绒山羊场羊只存在的膘情差、肋骨开张不良、四肢不端正、成年羊早期掉牙、青年羊牙齿发育迟缓和角尖破损严重等现象,对该场羊只的日粮组成和管理方法进行详细调查,并随机采取20只5~6岁母羊血样,测定了血清中的钙、磷、三碘甲腺原氨酸(T3)、甲状腺素(T4)水平以及碱性磷酸酶活性(ALP).结果表明:该场绒山羊(Ⅰ组)饲料单一、品质差、霉变现象较严重,日粮钙磷比例严重失调,而且饲养管理方法较落后.羊只血清磷水平为1.79 mmol/L,而来自另一羊场的健康羊(Ⅱ组)血清磷水平为1.95 mmol/L,二者差异显著(P<0.05).Ⅰ组羊血清ALP活性达到(457.75±606.86) U/L , 是Ⅱ组(95.80±60.09) U/L的4.78倍,其差异极显著(P<0.01).虽然两组血清T3水平差异不显著,但Ⅰ组血清T4水平明显高于Ⅱ组,分别为8.72±1.61和(8.08±1.31)μg/dL,(P<0.05).由此可见,该场羊只患有严重的骨质疏松症,日粮钙磷比例失调是导致骨质受损的主要因素. 相似文献
56.
研究了钙- 钙调素(Ca2+-CaM) 对水杨酸( SA) 诱导葡萄幼苗耐热性的影响, 以及抗氧化酶、MDA、CaM和Pro在这一过程中的变化。结果表明: (1) 外源SA可提高葡萄幼苗的耐热性, 而Ca2+可促进SA对耐热性的诱导。但是, Ca2+螯合剂EGTA、Ca2+通道抑制剂La3+以及CaM拮抗剂W7对SA诱导的耐热性产生抑制作用。高温热激后, SA或SA加Ca2+处理促进叶片内CaM的积累, EGTA、La3+或W7则抑制CaM的积累。(2) 高温下, SA通过维持高水平的SOD和CAT的活性, 降低MDA含量来抵抗高温造成的氧化胁迫; 外源Ca2+可促进SA对SOD和CAT的诱导, 而EGTA、La3+或W7则产生相反的作用。高温前后, 各处理叶片内的POD和APX的活性并没有明显的变化。(3) SA或SA加Ca2+处理可增加叶片中的Pro含量, 并在高温下维持较高的水平。高温后, 各抑制剂处理叶片中的Pro含量与对照无明显差异。结果表明, Ca2+可调控SA诱导的耐热性, 而且在此过程中, 要求细胞外的Ca2+穿过质膜进入胞内, 并有抗氧化酶和Pro的参与。 相似文献
57.
AIM:To investigate the effect of the heat shock response on the reperfusion arrhythmias(RAs) and the possible mechanism involved. METHODS:Fifty-five Sprague Dawley rats were randomly divided into 2 groups: the heat shock group (group H,n=29) and the control group (group C,n=26). The rats in group H were preconditioned with heat shock 24 hours before, and that in group C were not. The hearts of 16 rats in group H and 16 in group C were exercised and mounted on a non-circulating Langendorff perfusion apparatus and perfused retrogradely with modified K-H buffer and mimic ischemia/reperfusion was applied. Additionally, conventional intracellular microelectrode techniques were used for recording such electrophysiological parameters as resting potential(RP), action potential amplitude(APA), over shot(OS), maximum depolarization velocity(Vmax) of the hearts of other 13 rats in group H and 10 in group C. RESULTS:①Prior heat stress significantly decreased reperfusion arrhythmia. ②The amount of CK release in the effluent in group H was much less than that in group C. ③Myocardial HSP70 content was elevated significantly in group H. ④Heat stress significantly increased myocardial anti-oxydases activity and decreased lipid peroxydative products. Additionally, heat stress significantly reduced the Vmax of action potential. It indicated that rapid Na+ channel of papillary muscles may be inhibited by heat shock. The degree of change of Vmax after ischemia in H group was significantly less than that in group C. And the time of reperfusoin with Tyrode's solution till the action potential appeared as large as that before perfusion with mimic ischemic solution is shorter in group H than in group C. CONCLUSION:Heat shock pretreatment markedly reduces ischemia/reperfusion-induced injury of heart and ventricular arrhythmias in rats and this effect may be associated with the inhibition of rapid Na+ channel of papillary muscles by heat shock and the increase in myocardial HSP70 and anti-oxydase activity. 相似文献
58.
59.
AIM:To investigate the effect of high concentration of chlorine gas(Cl2) exposure on lung haemodynamic and respiratory function in intact and isolated perfused rabbit lungs (IPLs). METHODS:8 intact and 10 IPLs were exposed to Cl2 at high concentration(50×10-4)for 20 min, as measured group, 8 additional intact and 9 IPLs, which were similarly treated but not exposed to Cl2, served as controls. The changes of lung weigh of IPL(△W)、pressure of pulmonary artery(Pa)and venous pressure(Pv)、airway pressure and tidal volume(TV) were continuously measured and recorded simultaneously. RESULTS: In IPL group: While the perfusing blood flow was kept constant (133.3 mL/min), and Pv did not change, following the exposure, the Pa increased slightly, then the lung weight were increased significantly and the TV decreased . Hematocrit of perfusate of EIPL and parameters of lung water increased also. In intact group: Pa increased slightly, respiratory rate accelerated immediately, and TV decreased. CONCLUSION:Although mean Pa increased continuously and slightly in both intact and IPL group following the exposure to high concentration of Cl2, the primary cause of edema was most likely to alter pulmonary capillary permeability. The respiratory rate accelerated and TV decreased due to exposure to Cl2 enhanced hypoxia of intact rabbits. 相似文献
60.
AIM:To explore the changes of L-selectin expression on peripheral blood polymorphonuclear leukocytes (PMNs) and their significance in rats with acute lung injury (ALI). METHODS:ALI model in rat was established by intravonous injection of E. coli endotoxin (ET). The expression of L-selectin on peripheral blood PMNs was measured by immunofluorescence and flow cytometry.RESULTS:The contribution of L-selectin fluorescence signal was on the surface of PMNs membrane. The expression of L-selectin on poeripheral blood PMN was significantly lower at 5 min after injection of ET and the lowest during 15 min to 30 min, then gradually increased, but the expression of L-selectin on PMN was lower at 60 min after injection of ET than that of control animal.CONCLUSION:In physiological state, L-selectin were expressed on the surface of PMN membrane. The protein expression of L-selectin on PMNs reduced rapidly after injection of ET and the lowest at 15 min, then gradually increased. L-selectin may play a role in the development of ALI. 相似文献