Effects of histone hypomethylation induced by alcohol during pregnancy on overexpression of cardiomyogenesis genes offspring mice

AIM:To investigate the effects of histone methylation on the abnormal expression of cardiomyogenesis genes caused by alcohol during pregnancy and the regulatory mechanism, and to provide a new idea and intervention targets for preventing and curing congenital heart disease. METHODS:The alcohol (56%, 5 mL/kg) and G9a-histone methyltransferases (HMT) inhibitor BRD4770 (1 mg/kg) were given by gavage in Kunming mice during embryo (E) 0.5~14.5 d, and the hearts of the mice in E14.5, E16.5 and post neonatal 0.5 d (PND0.5) were collected. The mRNA expression of Gata4, Cx43 and β-MHC genes was detected by RT-qPCR. The activity of HMT was measured by colorimetry. Meanwhile, the protein expression of histone H3K9me3, G9a-HMT, Cx43 and β-MHC was determined by Western blot. RESULTS:The results of colorimetry showed that the activity of HMT in the heart of the offspring mice treated with alcohol during pregnancy was decreased significantly compared with normal saline group (P<0.05), and Western blot data showed that the expression of G9a-HMT and histone H3K9me3 were apparently decreased in the same samples (P<0.05). The mRNA expression levels of Gata4, Cx43 and β-MHC in alcohol group were apparently increased compared with normal saline group (P<0.05). Meanwhile, the protein levels of Cx43 and β-MHC were increased significantly in the same samples (P<0.05). However, BRD4770, a G9a-HMT inhibitor, further attenuated the level of histone H3K9me3, and further upregulated the expression of Gata4, Cx43 and β-MHC in the heart of the the mice treated with alcohol (P<0.05). CONCLUSION:Histone methylation modification imbalance induced by G9a-HMT may be involved in the abnormal expression of cardiomyogenesis genes in the heart of offspring mice caused by alcohol during pregnancy.     

辣椒抗番茄斑萎病毒病研究进展及展望

番茄斑萎病毒（Tomato spotted wilt virus,TSWV）自19 世纪发现以来,在全世界各地蔓延,对多种作物造成重大经济损失。本文以辣椒为例,从TSWV 的发生与分布、病害症状检测、传播方式、防治方法、抗TSWV 育种及展望等6 个方面对国内外辣椒TSWV 的研究现状进行综述。     

海南辣椒、樱桃番茄病毒病调查及病原dot-ELISA鉴定

[目的]调查和鉴定海南省辣椒和樱桃番茄病毒病发生情况和病毒种类,明确病毒病发生为害程度及毒源种类.[方法]2018—2019年,通过5点取样法对海南省10个市县的辣椒和樱桃番茄进行病毒症状类型、发病率和病情指数统计.采集辣椒和樱桃番茄主产区病毒病样本593份,通过斑点酶联免疫吸附法(dot-ELISA)对黄瓜花叶病毒(...     

小肽对大黄鱼仔鱼生长和小肠发育的影响及其在低温胁迫下的抗氧化应激效应

本试验分为生长试验和低温胁迫试验2部分.先分别投喂大黄鱼(Larimichthys cro-cea)仔鱼经不同浓度(0、0.5、1.0、2.0和3.0 mL/m3)小肽营养强化后的轮虫和卤虫12 d,以探讨小肽对大黄鱼仔鱼生长和小肠发育的影响;再将大黄鱼仔鱼暴露在温度为12℃的水体中24 h,以探讨小肽对低温胁迫下大黄...     

基于IRT框架的乡村旅游协同发展机制研究 ——以山东省典型村为例

乡村旅游产业发展契合了乡村振兴战略的基本要求,以可持续发展视角关注乡村旅游业的多重价值,为乡村产业融合、经济发展和农民就业增收提供了持久动力。基于一体化乡村旅游(IRT)框架理论,以山东省乡村旅游典型村为研究对象,运用案例分析方法探究乡村旅游协同发展的内在基础与阶段特征,剖析乡村旅游协同发展的驱动机制与实现路径。结果表明,乡村旅游初始发展阶段依赖于资源内生性与利益共享性,市场化运作阶段强调组织化嵌入与规模化经营,快速扩张阶段关注社区赋权与内生性发展,持续发展阶段诉求利益均衡与合作共生。社区参与、多元赋权与价值认同,多利益相关者的参与、联合与协同,持续、有效的制度性供给与规范是乡村旅游协同发展的运行基础。但是,乡村旅游不同发展阶段面临着多元主体利益不均衡、资源内生性不足、乡土文化异化、社区赋权程度低和城乡要素流动不畅等问题与挑战。因此,提出合作网络构建与协作经营机制、多元主体的利益协调机制、乡村社会文化的保护与建构机制、资本嵌入与竞合机制等协同发展路径。     

生计资本视角下农户生计策略变动及影响因素研究 ——基于CFPS四期追踪数据

农户的生计策略在乡村振兴进程中正在和即将发生变化,只有对农户生计策略的变化做出正确的判断,才能够有效引导农户生计行为,破除乡村建设行动的各种阻力。基于可持续生计理论,利用CFPS四期追踪数据,运用转移概率矩阵和Logit回归模型,分析农户生计策略变动及其影响因素,并运用分位数回归方法验证稳健性。结果表明,不同生计策略农户的生计资本结构存在差异,无论何种生计策略的农户,人力资本在5种资本中都最大。短期内多数农户选择维持原有生计策略,动态调整过程较慢,有土地转入的农户更倾向于选择非务工型生计策略。社会资本对农户趋务工型策略变动具有显著的正向影响,农户受教育年限和健康对生计策略变动具有正向影响。与东部地区农户相比,中西部地区农户更倾向于选择务工型生计策略。因此,应加大农村产业多样化的投入力度,促进农户策略转变速度;鼓励农民参与土地流转;建立健全农户参与农村基层社会治理机制,推动农户社会资本积累;扩大对农村教育与医疗的投入力度。     

耕地细碎化、耕地质量认知与化肥施用效率 ——基于湖北省水稻种植户的调查数据

在中国倡导农业农村绿色发展的大背景下,化肥投入的政策导向逐渐由增量增产转变为减量增效,如何提高化肥施用效率成为当前重点关注的问题。本文依据湖北省水稻种植户微观调查数据,运用随机前沿法测算水稻种植户化肥施用效率,并在此基础上运用CLAD模型进一步分析耕地细碎化程度与耕地质量认知对化肥施用效率的影响。结果表明:当前水稻种植户化肥施用效率仅为63.85%,若完全消除效率损失,化肥施用效率仍有36.15%的提升空间;耕地细碎化程度显著降低了化肥施用效率,可能与农户的避险行为有关;耕地质量认知显著促进了化肥施用效率提升,说明农户对耕地质量的科学认识能够改善化肥施用效率。因此,提出加快耕地整合与耕地连片经营法律法规建设,加速耕地细碎化治理经验与创新结合,加强引导农户对化肥施用的正确认知,推行科学种植生产模式以提升化肥施用效率的政策建议。     

Analysis of static structural mechanics of vertical axis wind turbine with lift-drag combined starting structures

In this paper the finite element analysis was carried out for a composite vertical axis wind turbine with lift-drag combined starting structures to ensure the structure safety of a vertical axis wind turbine(VAWT). The static and modal analysis of rotor of a composite vertical axis wind turbine was conducted by using ANSYS software. The relevant contour sketch of stress and deformation was obtained. The analysis was made for static structural mechanics, modal analysis of rotor and the total deformation and vibration profile to evaluate the influence on the working capability of the rotor. The analysis results showed that the various structure parameters lie in the safety range of structural mechanics as given in the relative standards. The analysis showed the design safe to operate the rotor of a vertical axis wind turbine. The methods used in this study can be used as a good reference for the structural mechanics′ analysis of VAWTs.