Effects of naringenin on PI3K/AKT signaling pathway and endoplasmic reticulum stress and its related apoptotic pathways in rats with myocardial ischemia/reperfusion injury

AIM To observe the effect of naringenin on cardiac injury in ischemia/reperfusion （I/R） rats, and to explore whether the role of naringenin is involved in PI3K/AKT signaling pathway and endoplasmic reticulum stress and its related apoptotic pathways. METHODS SD rats （n=48） were randomly divided into sham operation （sham） group, model （I/R） group, naringenin treatment （NAR） group and naringenin+LY294002 （NL） group. Myocardial I/R injury model was prepared by ligation of left anterior descending coronary artery of rats for 30 min followed by reperfusion for 120 min. After reperfusion, the serum levels of cardiac troponin I （cTnI） was measured by ELISA. HE staining, TTC staining and TUNEL staining were used to detect the myocardial histopathological changes, myocardial infarction area and myocardial cell apoptotic rate. The mRNA levels of endoplasmic reticulum stress-related indicators glucose-regulated protein 78 （GRP78）, C/EBP homologous protein （CHOP） and caspase-12 were detected by RT-qPCR. The protein levels of cleaved caspase-3, GRP78, CHOP, caspase-12, p-PI3K and p-AKT were determined by Western blot. RESULTS Compared with I/R group, the serum content of cTnI, myocardial pathological damage, myocardial infarction area and myocardial cell apoptotic rate were significantly reduced （P<0.05）, the protein levels of cleaved caspase-3, GRP78, CHOP and caspase-12 were decreased （P<0.05）, and the protein levels of p-PI3K and p-AKT were increased in NAR group （P<0.05）. LY294002 attenuated the protective effect of naringenin to some extent. CONCLUSION Naringenin reduces myocardial I/R injury in rats possibly by activating PI3K/AKT signaling pathway and subsequently regulating endoplasmic reticulum stress and its related apoptotic pathways.     

茶叶提取物对抗肺癌细胞(A549)体外试验研究

观察表没食子儿茶素没食子酸酯(EGCG)、茶黄素-3、3'-双没食子酸酯(TFDG)、茶黄素(TF)、茶黄素复合物(TFs)、葡萄籽提取物(GrapeSeedExtract,GSE)、松树皮提取物(PineBarkExtract,PBE)、咖啡因(Caf)、槲寄生(VCE)和茶氨酸(The)的体外抗癌活性,通过人肺癌细胞(A549)进行体外试验,结果表明除咖啡因、槲寄生、茶氨酸的作用较小以外其他几种均有很强的促进人肺癌细胞(A549)凋亡的作用,并且EGCG的作用最强,顺序为EGCG、TFs>GSE>PBE、TFDG>TF。用作图法求得EGCG、GSE、PBE、TFDG和TF的IC50值分别为1.01μM、21.91μM、31.01μM、32.87μM和279.67μM。     

宜昌市柑橘冻害发生的气象特点及柑橘不同品种在冻害中表现和冻后恢复比较

【研究目的】全球升温趋势之下,极端温度变幅加大和低温造成的灾害性天气频率不减反增,2008、2011和2012宜昌柑橘产区部分或大面积发生不同程度柑橘冻害。本研究旨在增强人们对于冻害发生天气情况的了解,为柑橘产区品种规划及防寒减灾提供参考资料【方法】通过对宜昌市夷陵区历次柑橘冻害发生的气象资料统计和冻后实际调研情况分析,对最近几年冻害发生过程中不同柑橘种类和品种的表现及冻后恢复情况进行比较。【结果】发现历次大冻均发生于1月5日至1月31日之间,冻害程度与低温强度及低温持续时间呈现正相关。比较了柚、杂柑、椪柑、温州蜜柑和甜橙等不同柑橘种类在冻害中的表现,结果表明,受冻程度由重到轻依次为：杂柑→柚类→橙类→椪柑→温州蜜柑,离大水体远的果园受冻严重;坡向、坡位对冻害程度有影响。冻后恢复情况与树体冻害程度和冻后恢复能力有关,甜橙和柚冻害较重,但恢复能力较强,椪柑受冻虽然较甜橙和柚为轻,但冻后恢复较慢,而温州蜜柑冻害发生轻、冻后恢复也最快。【结论】在柑橘栽培北缘地区,因地制宜选择适宜品种、合理进行规划布局对柑橘产业健康发展极为重要。     

UV-C处理对水蜜桃果实冷害及贮藏品质的影响

为了缓解水蜜桃果实采后冷藏保鲜对果实品质的负面影响,达到更好的保鲜效果,以江苏省张家港市的凤凰水蜜桃（Prunus persica)为实验材料,研究了不同剂量（0.5kJ/m2、1kJ/m2、1.5kJ/m2）UV-C对低温（3℃?℃）条件下水蜜桃果实冷害和品质的影响。实验结果表明：三种剂量的UV-C处理对水蜜桃均能有效减少果实的褐变,保存果实的可溶性固形物（TSS）,并明显延缓了丙二醛（MDA）、多酚氧化酶（PPO酶）的上升趋势;处理之间没有明显的效果差异,但1.5 kJ/m2处理的硬度值在5-20d之间均显著大于CK组（P<0.05）,表现出更好的抗软化能力;另外,UV-C处理易使果实相对电导率加速升高,该趋势随照射剂量的增大而增强。     

漯河地区小麦冻害发生原因及对策

小麦生长发育受冻害影响,而不良的环境条件严重危害小麦成长。冻害主要是指因温差骤降且时间过长影响小麦成长的情况。漯河地区冬季小麦冻害情况极为严重,而二类、三类麦田屡见不鲜,冬季冻害会造成极为严重的经济损失,只有针对其实际情况进行深入分析,并采取有效举措予以应对,即能够保证漯河地区小麦的稳定产量。     

热带果蔬采后冷害研究进展

果蔬采后冷害机制及其防控是当前采后研究领域中倍受关注的科技问题。果蔬冷害发生是由内在因子和外部环境因素共同作用的结果。近年来,随着现代分子生物学技术的应用,并借鉴模式植物拟南芥的研究成果,果蔬采后冷害发生调控机理及防控技术研究取得一系列进展。本文综述了热带果蔬采后冷害发生的生理与分子机制和冷害防控技术的最新研究成果,关注能量代谢调控果蔬采后冷害作用,重点介绍了生物技术在防控果蔬冷害上的应用,并对今后研究方向作了展望。     

山西南部苹果花期冻害时空分布特征及综合防御技术

利用1997—2016年山西南部苹果主产县(市、区)苹果花期逐日最低气温及日平均气温观测资料,根据苹果花期冻害等级划分指标,采用最小二乘法对花期冻害日数进行线性倾向估计,并用Mann-Kendall非参数检验方法对花期冻害日数的变化趋势进行显著性检验,分析山西南部苹果花期冻害的时空分布特征。结果表明: 山西省南部苹果产区发生花期冻害日数年均为3.1 d,各县花期均温介于13.0℃~13.8℃之间。运城地区4月中上旬易发生冻害,临汾地区吉县与隰县4月下旬易发生冻害;山西南部苹果花期冻害日数近20 a气候倾向率为-0.666 d·（10 a）^-1（P≤0.01）,花期极端最低气温气候倾向率为0.165 d·（10 a）^-1（P≤0.01）,花期极端最低气温与冻害日数具有极显著负相关关系。山西南部苹果花期冻害日数突变点在2008年,且在2015年之后苹果花期冻害日数突破α=0.05显著性水平下限。对于苹果花期冻害的综合防御措施,可采用“以防为主,抗补结合”策略。     

Expression and function of circular RNA_0000231 in non-small-cell lung cancer

AIM: To investigate the expression and function of circular RNA_0000231 (circ_0000231) in non-small-cell lung cancer (NSCLC). METHODS: RT-qPCR was used to detect the expression of circ_0000231 in the NSCLC tissues and cell lines. circ_0000231 small interfering RNA (si-circ_0000231) or negative control siRNA of circ_0000231 (NC) was transfected into the NSCLC cells. The proliferation and apoptosis of the NSCLC cells were detected by CCK-8 assay, colony formation assay and flow cytometry, respectively. The expression of cyclin D1 (CCND1) and anti-apoptotic protein Bcl-2 were determined by RT-qPCR and Western blot. RESULTS: The expression of circ_0000231 in the NSCLC tissues and cell lines was significantly up-regulated compared with precancerous tissues and lung epithelial cells BEAS-2B (P<0.05). After transfection of NSCLC cells with si-circ_0000231, the cell viability, colony formation numbers were significantly decreased, and the apoptotic rate in si-circ_0000231 group was significantly increased as compared with NC group (P<0.01). In addition, the results of RT-qPCR and Western blot showed that transfection of si-circ_0000231 inhibited the expression of CCND1 and Bcl-2 (P<0.01). CONCLUSION: The expression of circ_0000231 is significantly increased in the NSCLC tissues and cells. Knock-down of circ_0000231 expression significantly inhibits the proliferation of NSCLC cells.     

1958—2018年广西早稻高温热害时空变化规律

为了掌握广西水稻高温热害的变化规律特征,为水稻生产制定防灾减灾对策提供决策依据,本研究利用广西89个气象观测站1958—2018年观测资料及地理信息数据,采用GIS技术、M-K突变分析和Morlet小波周期分析等方法,探究广西早稻高温热害发生的时间变化规律和空间分布等特征。结果表明：（1）1958—2018年广西早稻高温热害呈增长趋势,20世纪90年代为发生低谷期,21世纪10年代为高发期;（2）高温热害发生次数在2009年发生增多突变,除重度高温热害外,轻、中度高温热害次数变化均有突变发生;（3）高温热害发生频次存在18、32年左右的显著周期变化,预计2018年之后广西早稻高温热害仍处于高发期;（4）高温热害高发区位于左、右江河谷,桂中盆地和西江—浔江—郁江等流域区域,沿海地区发生频次少于内陆。以上研究结论对实现水稻丰产稳产优产具有重要的现实意义。