木霉L24菌株的农用生物活性初步研究

抑制菌丝生长速率法生测结果表明:木霉L24菌株水溶性代谢物对供试菌株均有较好的抑制作用,抑制率均在50%以上;菌丝互作试验结果表明,木霉L24菌株菌丝对茄丝核菌菌丝有重寄生作用;盆栽试验结果表明,木霉L24菌株对小白菜和萝卜幼苗生长均有很好的促进作用,促进率分别为215.1%和166.3%;大田试验结果表明,木霉L24菌株对油菜苗期的生长也具有良好的促进作用。     

陇春24小麦品种的丰产性和稳定性分析

[目的]为了全面了解陇春24小麦品种的丰产性能。[方法]依据2004～2005年甘肃省水地春小麦区域试验资料,运用联合方差分析法和高稳系数法(HSC),对陇春24和其他21个品种的丰产性和稳产性进行了分析,同时利用灰色关联度分析法对2004￣2005年小麦区域试验中相同地点的4个品种进行了综合评价。[结果]联合方差分析结果表明,2004年区域试验中,陇春24平均产量为8624.40 kg/hm2,较对照高原602增产6.65%;2005年区域试验平均产量为7 627.35 kg/hm2,较对照增产11.65%。HSC分析表明,陇春24、91043的高稳系数与产量间的相关系数为-0.8515,高稳系数与变异系数的相关系数为0.7845,高稳系数与标准差间的相关系数为0.4806。[结论]陇春24小麦品种丰产稳产性好,具有较大的增产潜力,在甘肃河西及沿黄灌区适应性较好。     

不同栽培方式对大豆丰收24产量的影响

为了探寻适合于大豆丰收24的最佳栽培方式,连续2 a以不同垄距、不同密度为试材,进行不同栽培方式对大豆丰收24产量影响的研究.试验结果表明:22.5 cm平作37万株/hm2和45 cm垄距40万株/hm2两个处理组合为本试验中丰收24的最佳栽培方式.     

表油菜素内酯对冬小麦开花后光合特性和物质生产的影响

为明确表油菜素内酯对小麦生育后期光合特性的影响和最适应用时期和浓度,以冬小麦品种‘石麦18’为材料,设置起身期、起身期+孕穗期、孕穗期3个喷施时期为主区,0~0.15mg/L 4个表油菜素内酯喷施浓度(每次喷施量673.8kg/hm2)为副区的裂区试验,测定了开花后旗叶的净光合速率(Pn)、气孔导度(Gs)、胞间CO2浓度(Ci)、蒸腾速率(Tr)、叶绿素SPAD值,以及成熟期植株和籽粒干物质量。结果表明:各处理小麦旗叶的Pn、Gs、Ci、Tr和叶绿素SPAD值从开花期开始均逐渐提高,叶绿素SPAD值开花后10d达到最高值,Pn等4项光合参数在开花后15d达到最高值,然后开始下降。不同处理比较,起身期1次喷施、起身期+孕穗期2次喷施的,4种浓度之间光合参数、叶绿素SPAD值在各测定日期基本上都是0.1mg/L0.05mg/L0.15mg/L对照,且0.1mg/L与其他浓度的差异显著;孕穗期喷施的则是0.05mg/L0.1mg/L0.15mg/L对照,且0.05mg/L与其他浓度的差异显著。各测定日期的光合作用参数和叶绿素SPAD值均以起身期+孕穗期2次喷施0.1mg/L的最高。处理之间光合作用参数的差异,比处理之间叶绿素SPAD值的差异更大。各处理之间总干物质和籽粒干物质量的差异,与光合参数和叶绿素SPAD值的差异一致。表油菜素内酯喷施浓度对干物质量的影响大于喷施时期的影响,对总干物质量的影响大于对籽粒干物质量的影响。综合以上结果,应用表油菜素内酯调控小麦光合性能和物质生产,以起身期+孕穗期2次各喷施0.1 mg/L水溶液670kg/hm2左右为宜。如喷施1次,起身期以0.1mg/L、孕穗期以0.05mg/L为宜。     

Development and Validation of SCAR Markers Co-Segregating with an Agropyron Elongatum Derived Leaf Rust Resistance Gene Lr24 in Wheat

Summary An Agropyron elongatum-derived leaf rust resistance gene Lr24 located on chromosome 3DL of wheat was tagged with six random amplified polymorphic DNA (RAPD) markers which co-segregated with the gene. The markers were identified in homozygous resistant F₂ plants taken from a population segregating for leaf rust resistance generated from a cross between two near-isogenic lines (NILs) differing only for Lr24. Phenotyping was done by inoculating the plants with pathotype 77-5 of Puccinia triticina. To enable gene-specific selection, three RAPD markers (S1302₆₀₉, S1326₆₁₅ and OPAB-1₃₈₈) were successfully converted to polymorphic sequence characterized amplified region (SCAR) markers, amplifying only the critical DNA fragments co-segregating with Lr24. The SCAR markers were validated for specificity to the gene Lr24 in wheat NILs possessing Lr24 in 10 additional genetic backgrounds including the Thatcher NIL, but not to 43 Thatcher NILs possessing designated leaf rust resistance genes other than Lr24. This indicated the potential usefulness of these SCAR markers in marker assisted selection (MAS) and for pyramiding leaf rust resistance genes in wheat.     

基于Wi—Fi的自动灌溉控制器设计与实现

针对传统的灌溉控制器布线多、施工难,且采用RS485与计算机通信时距离受限等问题,设计和实现了一个基于wi—Fi的自动灌溉控制器,能在远程计算机上以Web网页的方式实时控制灌溉、显示监测信息。该控制器可同时测量8路模拟量、3路数字量,并可以控制7路交流电磁阀。该灌溉控制器具有友好的监测操作界面,并且实现了广泛的信息共享。因此,在农业领域具有广阔的应用前景。     

春秋兼用蚕品种57A·57B、24·46的育成及其四元杂交种的选配

采用杂交育种方法,在春、夏、秋不同环境下定向培育,经严格的后代选择,育成二个中系限性品种57A、57B和二个日系品种24、46,然后运用杂种强势的原理,选配成四元杂交种57A·57B×24·46.经多次鉴定结果表明,该四元杂交种的产量、产值比对照种提高3—4%;且体质强健,对叶质要求不高,制种较容易,丝质优良,是春秋兼用的优良蚕品种.     

铁皮石斛DoAGL6基因及启动子克隆与分析

AGL6基因是MADS-box转录因子家族中的一员,是植物特有的转录调控因子,参与花器官形成,对唇瓣的形成起到关键作用。获得铁皮石斛DoAGL6基因及其启动子,并进行生物信息学分析,对DoAGL6基因启动子进行瞬时表达活性验证,可为进一步研究DoAGL6基因的功能提供参考。本研究克隆DoAGL6基因及其上游的启动子序列,利用在线软件对克隆得到的目的基因序列、启动子序列进行预测,分别构建由全长启动子和5'端缺失启动子驱动GUS的重组表达载体并瞬时转化拟南芥及铁皮石斛原球茎,探究其表达活性。结果表明,成功克隆了DoAGL6基因及其启动子,DoAGL6基因编码区长729 bp,编码243个氨基酸,编码蛋白分子式为C₁₂₁₃H₁₉₅₅N₃₅₉O₃₇₅S₁₂,预测亚细胞定位于细胞核中;保守结构域分析表明,DoAGL6具有保守的MADS-box和K-box两个结构域,属于MADS基因家族MIKC亚家族。启动子序列长度为1891 bp,顺式作用元件分析结果显示,DoAGL6基因启动子中除了核心启动元件TATA-box、CAAT-box外,还有许多其他顺式作用元件,如脱落酸响应元件（ABRE）、光反应顺式调节元件（G-Box）、茉莉酸甲酯响应元件（TGACG-motif）、MADS-box蛋白结合位点（GArG-Box）等。成功构建融合表达载体pCAMBIA1300-A1-promoter::GUS、pCAMBIA1300-A2-promoter::GUS、pCAMBIA1300-A3-promoter::GUS,拟南芥和铁皮石斛原球茎瞬时转化及GUS组织化学染色结果表明,随着启动子5'端缺失片段的增长,GUS活性逐渐降低,启动子活性逐渐减弱,即全长启动子A1（-1891~1）的活性最强,5'端缺失片段A2（-1488~1）次之,A3（-784~1）活性最弱。瞬时转化后的拟南芥和铁皮石斛原球茎GUS染色均呈现蓝色,但与对照相比均较浅,说明全长启动子和2个5'端缺失启动子都具有驱动GUS的活性,但启动活性都比CaMV35S启动子的启动活性弱。     

Dendritic cells transfected with recombinant adenovirus carrying IL-24 gene induces CTLs to promote apoptosis of cervical cancer cell line CaSki

AIM：To investigate the therapeutic potential of interleukin-24 (IL-24) in cancer treatment, we observed the inductive effect of cytotoxic T-lymphocytes (CTLs) against human cervical cancer cell line CaSki using dendritic cells (DCs) transfected with recombinant adenovirus carrying IL-24 gene (Ad-IL-24-DCs). METHODS：Immature mouse DCs were isolated and cultured. The DCs were infected with recombinant adenovirus carrying IL-24 gene. The CaSki cell lysate-loaded autologous DCs vaccine was also prepared. The cell proliferation was analyzed by MTT assay. The cell apoptosis was determined by flow cytometry with PE-Annexin V staining. The cleaved caspase-3 in the Ad-IL-24-DCs was measured by Western blotting. Colony-forming assay was used to detect the colony number of Ad-IL-24-DCs. The tumorigenic capacity of CaSki cells in vivo in the presence of Ad-IL-24-DCs was observed by tumor-burdened model. RESULTS：IL-24 was highly expressed in the Ad-IL-24-DCs. In the presence of Ad-IL-24-DCs, the apoptosis of CaSki cells was significant increased. Additionally, the protein level of cleaved caspase-3 was increased in CaSki cells in the presence of Ad-IL-24-DCs, suggesting that the CaSki cells with Ad-IL-24-DCs induced apoptosis. CTLs induced by Ad-IL-24-DCs inhibited the tumorigenic capacity of CaSKi cells in vivo. CONCLUSION：Genetically modified DCs are successfully prepared by infection with Ad-IL-24 and show a significant effect on triggering specific CTLs against CaSki cells.     

Role of miRNA-24 in regulation of endothelial nitric oxide synthase expression and vascular endothelial cell proliferation

AIM：To investigate whether miRNA-24 is involved in the regulation of endothelial nitric oxide synthase (eNOS) expression and vascular endothelial cell proliferation. METHODS：A plasmid that highly expressed miRNA-24 was constructed, and was transfected into the human umbilical vein endothelial cells (HUVECs) by liposome. The cell proliferation was detected by MTT assay. The expression of eNOS and Sp1 at mRNA and protein levels was exa-mined by real-time PCR, immunohistochemistry and Western blotting.RESULTS：Compared with control group, the proliferation of endothelial cells in miRNA-24 group was significantly decreased by 41.97 % (0.47±0.04 vs 0.81±0.03, P<0.01), and the expression of eNOS at mRNA and protein levels was decreased by 44.8% (0.48±0.01 vs 0.87±0.03, P<0.05) and 71.92% (0.16±0.06 vs 0.57±0.08, P<0.05), respectively. Meanwhile, the mRNA and protein levels of Sp1 were significantly decreased by 53.00% (0.45±0.02 vs 0.93±0.01, P<0.05) and by 62.31% (0.13±0.07 vs 0.31±0.09, P<0.05), respectively. In miRNA-24 inhibitor group, the above indexes were decreased compared with control group, but significantly increased compared with miRNA-24 group. CONCLUSION：miRNA-24 significantly inhibits the proliferation of HUVECs and the eNOS expression. Sp1 possibly acts as one of the important factors in the regulation of eNOS expression by miRNA-24.