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通过PCR方法自含NDVZF基因的克隆质粒中扩增NDVF基因 ,将其与真核表达载体pcDNA3..1/V5_His_TOPO重组。经核酸内切酶酶切 ,阳性克隆鉴定准确无误 ,核酸序列测定结果与原始基因比较 ,同源性大于 99% ,启始密码和终止密码未出现变异 ,将该重组质粒命名为pcDNANDVZF。将pcDNANDVZF在脂质体作用下转染CEF细胞 ,用间接免疫荧光试验检测 ,结果证明pcDNANDVZF可在CEF细胞中大量表达F蛋白。 相似文献
4.
大豆室内耐冷筛选及其在田间的应用研究 总被引:4,自引:1,他引:3
本试验把生长箱大豆耐低温萌发筛选与田间早春播研究相结合,验证耐低温发芽的可靠性。在实验室,萌发期表现抗冷的大豆,田间出昔期仍表现抗冷或较抗冷。实验表明,大豆出苗的生物学最低温度平均为7.1℃,所需有效积温136℃。地温的高低直接影响大豆早春出苗的多寡,两者呈密切的正相关。黄、青、黑豆中,以黑豆抵抗早春不良气候条件的能力最强。 相似文献
5.
S. Leibovitch B. L. Ma Wanga E. Maloba D. L. Smith 《Journal of Agronomy and Crop Science》1992,169(3):209-215
Aspects of intensive management practices such as high-yielding cultivars, narrow-row spacings and fungicide treatments could potentially increase cereal yields in regions with a short crop-growing season and occasionally dry and hot weather. A field experiment was carried out at McGill University, Canada for three years (1987 to 1989) to test the effects of triadimefon (1-[4-chlorophenoxy]-3,3-dimethyl-1-[1H-1,2,4-triazol-1-yl]-2-butanone, or Bayleton, a trade name) fungicide (0 vs. 140 g a.i. ha−1 ) and row spacing (10 vs. 20 cm) on the yield components, yield and other agronomic traits (spike emergence, days to maturity, leaf disease and plant height) of spring barley ( Hordeum vulgare L.) cv. Cadette, Laurier and Leger. A cultivar by row spacing interaction resulted in a 11 to 13.5 % increase in grain yield due to narrow rows for Laurier in two out of the three years and up to 16 % for Leger in one year. Over the three years grain yields were on average increased 6 to 12 % due to use of the narrower row spacing. Fungicide application to barley at the early heading stage effectively controlled leaf diseases without influence on yield components or grain yield. Our results indicate that some components of intensive management such as narrow row spacing can be applicable in regions with a short crop-growing season. 相似文献
6.
进行了由氯化苄与甲苯合成苄基甲苯的工艺研究,筛选了精制方法.合成和精制后的油品,检测了理化电性能,作成模型电容器进行各种试验,表明该绝缘油具有优异的性能. 相似文献
7.
This paper depicted the physiographic landscape features and natural vegetation situation of study area (the eastern Jilin
Province), and expatiates the definition, basic characters and its development of Ecological Land Classification (ELC). Based
on the combination of relief map, satellite photography for study area and vegetation inventory data of 480 sample sites,
a 5-class and a 15-class ecological land type map was concluded according to 4 important factors including slope, aspect,
vegetation and elevation. Ecological Classification System (ECS) is a method to identify, characterize, and map ecosystems.
The Ecological Land Type (ELT) was examined and applied initially in eastern Jilin Province.
Foundation item: This paper was supported by Chinese Academy of Sciences “100 people” project and the Open Research Station of Changbai Mountain
Forest Ecosystem.
Biography: XIAO Bao-ying (1974-), female, postgraduate in Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016,
P. R. China
Responsible editor: Zhu Hong 相似文献
8.
本文报道了江西三种危害食用菌的绿色霉菌的生物学特性。它们是:绿色木霉(Trichoderma Viride pers exFn)。产黄青霉(Penicillium Chrysoenum Thom)和构巢曲霉(Aspergillus nidulcms(Eidzm)Winter)。 相似文献
9.
宿根花卉的特点及其在园林中的配置 总被引:3,自引:0,他引:3
根据宿根花卉种类繁多、色彩丰富、生长稳定、抗逆性强的特点,其在园林中的配置,宜于建花坛、花境,也可群植,与背景树相结合,还可作地被植物代替草坪,也可建单类花卉园. 相似文献
10.
John W.PATRICK 《浙江大学学报(农业与生命科学版)》1990,(4)
The instablity of the stimulation of photosynthate unloading from seed coats of Phaseolus vulgaris by abscisic acid (ABA ) and benzylamino purine ( BAP ) was studied in terms of experimental conditions. ABA stimulated photosynthate unloading at pH 6 and pH 8 without close dependence upon pH. ABA at a concentration range of 10-5-10-4 mol/L displayed stimulatory effect. However, BAP revealed no effect at a concentration range of 10-6-10-4 mol/L. Experiments designed with different transport time of 14C-photosynthate indicated that ABA might act at the plasma membrane of the unloading cells. Reducing endogenous ABA level by diminishing leaf area did not facilitate the manifestation of exogenous ABA function. Potassium ion stimulated photosynthate unloading from seed coats with highest promotion at 100 mmol/L K . However, no dependence of ABA stimulation of photosynthate unloading on K was found. 相似文献