基于Proteus的高精度存储式温室温湿度测量仪

为了降低高精度存储式温湿度测量仪开发成本和周期,采用Proteus软件辅助,进行高精度存储式温室温湿度测量仪的开发。此测量系统利用SHT75传感器进行温湿度数据采集,采用AT24C1024、DS1302保存数据和提供时钟,同时采用单片机的串行口把存储的数据发送到计算机,计算机程序采用VB编写。     

75%苯磺隆·双氟磺草胺WDG防治小麦田阔叶杂草效果及其安全性评价

筛选出高效除草剂是有效防治麦田主要杂草播娘蒿与荠菜的前提。采用随机区组试验设计方法,在田间测定75%苯磺隆·双氟磺草胺水分散粒剂(water dispersible granule,简称WDG)对小麦田播娘蒿与荠菜的防治效果和对小麦的安全性。结果表明,施药后45 d,75%苯磺隆·双氟磺草胺WDG 33.75、39.38、45.00、78.75 g a.i./hm~2剂量下对2种杂草总株数防效为66.6%、77.6%、78.1%、80.5%,总鲜质量防效分别为95.8%、96.5%、97.4%、97.7%,均高于对照药剂75%苯磺隆可湿性粉剂(wettable powder,简称WP)22.50 g a.i./hm~2和50 g/L双氟磺草胺悬浮剂(suspension concentrate,简称SC)4.50 g a.i./hm~2对杂草的防效。75%苯磺隆·双氟磺草胺WDG 4个处理区小麦的穗数、穗粒数、千粒质量和产量均显著高于空白对照区,除33.75 g a.i./hm~2处理外,其他处理区小麦增产5%以上。综上所述,苯磺隆·双氟磺草胺WDG推荐剂量为33.75 g a.i./hm~2,对小麦田播娘蒿与荠菜均有较好的防效,对小麦安全。     

大豆蚜Aghsp75基因对吡虫啉及温度胁迫的应激表达

热激蛋白在昆虫抵御温度胁迫和药剂胁迫反应中具有重要作用。本试验通过高通量测序获得一条大豆蚜热激蛋白基因的cDNA序列，该序列全长2982 bp，含1个长度为2052 bp的开放阅读框，编码683个氨基酸组成的多肽，多肽等电点约为6.30，分子质量约为77.8 kDa；推导的氨基酸序列与棉蚜Aphis gossypii HSP75同源性高达99.12%，属于hsp 75家族。我们把该基因定名为Aghsp75，已提交至GenBank（登录号为MN068810）。Aghsp75通过不同浓度吡虫啉药剂和不同温度胁迫成蚜后发现，经LC₅₀吡虫啉浓度胁迫3、6和24 h时以及在LC₃₀浓度吡虫啉胁迫12 h时该基因表达量显著升高；经6℃处理6 h时以及36℃处理3 h和6 h时该基因表达量显著升高。本研究表明该基因可能参与大豆蚜的抗逆过程，为利用分子生物技术手段防治大豆蚜提供理论保障。     

家蚕核型多角体病毒ORF 75基因的克隆及表达

根据GenBank发表的序列(L33180)设计引物,利用PCR技术扩增了编码家蚕核型多角体病毒(Bombyxmori Nucleopolyhedrovirus,BmNPV)ORF75基因的DNA片段,将其连接至pMD18-T载体上,获得了该基因成熟蛋白阅读框序列.利用设计的酶切位点将目的基因从克隆载体上切下.表达质粒pGEX-4T-2经BamHI/XhoI双酶切,与目的片断连接,得到重组表达质粒pGEX/Bm75.将重组表达质粒pGEX/Bm75转化入大肠杆菌BL 21中,经IPTG诱导表达后,12%SDS-PAGE分析表明产生57 kDa左右的特异蛋白质条带.对表达产物进行Westernblotting分析,结果表明,pGEX/Bm75经IPTG诱导产生的57 kDa蛋白条带与抗体发生了很强的交叉反应,融合蛋白得到了表达.     

Genetic Characteristics of Frizzled and Incomplete Frizzled Feather and SNP Detection of KRT75 Gene

The study was mainly focused on the genetic characteristics of frizzled feather trait,the phenotype difference between frizzled feather（FF）and incomplete frizzled feather（IFF),and SNP analysis of the candidate gene KRT75 in the frizzle and incomplete frizzle chickens of different feather colors,which could provide scientific gist for the frizzle gene mapping,development and utilization of the two kinds of chicken.The resource population（homozygous Yellow feather Kirin chicken×Huaixiang chicken）of hybrid F2 was established.The back,neck,wing primary and wing-bow feather were observed and the scatter diagram of rachis bending degree was drawn.The blood of 10 white frizzle feather Kirin（WFF）chicken,10 Yellow frizzle feather Kirin（YFF）chicken,10 Royal,25 Yellow feather incomplete frizzle（YFIF）chicken and 15 Black feather incomplete frizzle（BFIF）chicken from homozygous WFF chicken×Royal chicken of hybrid F1 were extracted,then the DNA of those samples were extracted.The primer of KRT75 was designed,the PCR products were also sequenced after purification,Chromas 2.22 and DNAMAN software were used to analysis of sequence peaks photos and sequence alignment,respectively.The amount of orthogonal and reciprocals cross in hybrid F1 was 127 and 139, respectively, which all were the slight（incomplete）frizzled feather phenotype,with no different penetrance.The resource population of hybrid F2 were consisted of 55 frizzle chicks including some hens and cocks,106 incomplete frizzled and 68 contour feather chicken,the proportion of which was coincided with Mendelian segregation ratio of 1:2:1 by χ²-test（P>0.05),therefore it preliminary verified that the frizzle gene F was autosomal incomplete dominant inheritance.FF and IFF curved upward deviating from the skin,and the line slope of trend line of FF was 3.5 times than that of IFF.It found that there was no 69 bp deletion mutation in exon 5 region of KRT75 gene in WFF and YFF chicken,but 3 SNPs（T71C,T83C,C95T）were deleted in the 69 bp,which were homologous CC in WFF and YFF chicken,of two were heterozygous in YFIF chicken,while all were heterozygous in BFIF chicken.2 SNPs（T662C and T770C）were also deleted in exon 6,heterozygous of which were only deleted in BFIF chicken.The haplotype analysis indicated that 9 haplotypes were detected in 60 individuals,hap1/hap1 was specific genotype of WFF and YFF chicken.The haplotypes of two incomplete frizzle feather chicken were apparently different,hap4 was specific haplotype of YFIF chicken,while hap6,hap7,hap8 and hap9 were specific haplotypes of BFIF chicken.The frizzle gene F was autosomal incomplete dominant inheritance,there were obvious differences between FF and IFF traits,5 SNPs in exon 5 and 6 of KRT75 gene probably were the reference of molecular markers as distinguishing between frizzle and incomplete frizzle chicken.     

果蔬膨化干燥监测系统的设计

采用单片机、SHT75温湿度传感器和压强传感器,对果蔬膨化干燥过程所需的温度、压强与水分含量进行监测,可降低果蔬干燥的成本,简化膨化干燥的过程。为此,设计了由SHT75温湿度传感器、压强传感器、DS1302时钟芯片、存储芯片AT24C512、按键及显示电路组成的果蔬膨化干燥监测系统。     

肟菌酯·戊唑醇75%水分散粒剂防治稻瘟病试验初报

田间药效试验结果表明,肟菌酯·戊唑醇75%水分散粒剂对水稻稻瘟病有较好的防治效果,其药效随用药量加大而显著上升,其中第2次药后15 d 17.5 g/667m2的防效达79.41%,与对照药剂拿敌稳15.0 g/667m2和43%戊唑醇SC 15.0 mL/667m2的防效相当,且安全性好,因此可以在生产上加以推广应用。     

基于SHT75温湿度传感器的红薯育苗监测系统研究

采用C8051F120单片机设计并实现了一个温湿度监测系统。该系统采用SHT75温湿度传感器实现育苗室中温湿度的检测,满足温湿度测量精度的要求;同时在SHT75温湿度传感器数据传输过程中进行CRC数据校验,从而保证了测量数据的可靠性;并实现了相对湿度、温度与露点的实时数显功能。该系统控制温度范围广泛、可靠性强、使用灵活,达到了预期效果。