辽东楤木根插育苗实验

经过在辽宁瓦房店和沈阳对辽东楤木进行6年的实验,其抗旱,耐瘠薄能力很强,生长状态良好。为寻求新的育苗前途,进行了高床、低床、大垅与根断平埋、直播和生长素浸根10个组合的实验,结果表明:低床直插成苗率达90%,如再配合磷霉素浸根,扦插成苗率达95%,可用于指导苗木生产。     

猕猴桃果实腐烂病田间药效试验

用不同浓度的70%甲基硫菌灵可湿性粉剂、50%咪鲜胺锰盐可湿性粉剂、75%肟菌·戊唑醇水分散剂、5%己唑醇微乳剂、10%苯醚甲环唑水分散粒剂及施药时间、不同施药次数对猕猴桃腐烂病进行田间药效防治试验。结果表明:己唑醇1 000倍、1 500倍和2 000倍3个处理防治效果分别为81.1%、59.2%和42.5%;用己唑醇1 500倍的施药时间和次数试验结果表明,谢花后一周内施药的处理防治效果均在60%左右,显著高于延后一周施药的处理,施药次数对防治效果影响不大,喷药3次和4次的防治效果均为59%左右,喷药2次防效较差,但与前两者差异不显著;5种杀菌剂药效试验结果表明,75%肟菌·戊唑醇水分散剂防效最好,为71%,显著高于其他处理,其次为咪鲜胺锰盐、己唑醇,防治效果分别为63.5%和52.4%,苯谜甲环唑和甲基硫菌灵防效较差均不足30%。     

降压草的组织培养技术研究

以白城市林业科学研究院选育的新品种降压草2号为试材,利用其带芽茎段进行组织培养,筛选降压草组织培养程序及培养基配方。结果表明:适宜的腋芽诱导培养基为MS+6-BA0.5mg L-1+NAA0.1mg L-1,适宜的增殖培养基为MS+6-BA1.0mg L-1+NAA0.5mg L-1+IAA0.1mg L-1。     

不同性状蜂群群势增殖和蜂蜜产量比较研究

为了弄清不同性状蜂群群势的增殖效果和蜂蜜产量。从2014年2月14日到5月21日用测子框对美意为父本与蜜浆高产为母本F1代蜂群和蜜粉高产F1代蜂群、东北黑蜂F1代蜂群测定群势,比较蜂蜜产量。结果表明:3个不同性状蜂群在卵虫、蛹和成蜂脾数上增殖明显,但三者之间增殖情况差异不显著(p0.05)。东北黑蜂F1代蜂群产量较高,显著高于蜜粉高产F1代蜂群(P=0.039),但美意为父本与蜜浆高产为母本F1代蜂群和蜜粉高产F1代蜂群之间差异不显著(P=0.460)。由此可知3种不同性状的蜂群增殖较明显,但东北黑蜂在生产蜂蜜时有着不对称优势,结果可为蜜蜂育种提供理论依据。     

鮰爱德华菌外膜微孔蛋白ompN1、ompN2和ompN3基因生物信息学分析及截段基因的原核表达与其抗原性检测

根据GenBank中鮰爱德华菌(Edwardsiella ictaluri)外膜微孔蛋白N(outer membrane porin protein,OMP)基因序列(NC_012779.2)设计引物,以鮰爱德华菌Ms12基因组DNA为模板,经PCR扩增后克隆至pMD19-T载体,将阳性克隆菌送公司测序。测序结果通过生物信息学软件对ompN1、ompN2和ompN3序列的氨基酸序列相对分子质量、分子式、信号肽、跨膜区、亲/疏水性、B细胞表位进行预测,并构建系统发育树;根据去信号肽的B细胞表位相对集中区域设计特异性引物,经PCR扩增后,将截段序列克隆至pMD19-T载体,鉴定成功后克隆至原核表达载体pET32a(+)并转化至BL21(DE3)/Rosseta(DE3)进行IPTG诱导表达,最后用兔抗鮰爱德华菌血清对重组蛋白OmpN1、OmpN2和OmpN3进行免疫原性检测。结果显示扩增得到ompN1(1 143bp)、ompN2(1 056bp)和ompN3(1 053bp)的序列,获得的基因登录号分别为KJ831564、KJ831565和KJ831566。经生物信息学分析显示,OmpN1、OmpN2和OmpN3蛋白分子式分别为C1940H2993N543O622S7、C1817H2723N497O579S5和C1896H2843N519O563S7,相对分子质量大小分别为44 100、40 900和41 800,均为含有1个信号肽和跨膜区的疏水性蛋白,具有1个革兰阴性细菌OM_channels超家族结构域,是1种嵌入型的膜外蛋白;SDS-PAGE电泳检测发现,诱导表达的融合重组蛋白均以包涵体的形式出现在沉淀中,大小分别约为61 800、58 700和59 100;Western-blot分析表明,重组蛋白OmpN1、OmpN2和OmpN3均能与兔抗鮰爱德华菌全菌血清结合,表明鮰爱德华菌外膜微孔蛋白N具有亚单位疫苗开发的潜力。     

浅析路桥工程过渡段施工技术的应用

随着社会经济的不断发展,路桥工程已经成为我国基础建设中的一项重要建设项目,过渡段施工作为路桥工程施工中的重要内容,其施工技术水平的高低直接关系着工程的整体质量。施工中应对过渡段施工加以重视,只有这样才能确保路桥工程施工的质量。本文主要对路桥工程过渡段施工中存在的问题、技术应用及注意事项进行了分析与探究。     

城市轨道交通段场大库内火灾探测器设置方案研究

本文根据城市轨道交通有上盖物业开发的段场库内的环境特点,提出线型光束感烟火灾探测器（传统线性光束感烟火灾探测器以及新一代双鉴成像式线性光束感烟火灾探测器）、管路采样吸气式感烟火灾探测器、图像型火焰探测器三种探测器设置方案,并对三种方案的技术性能进行了剖析,为城市轨道交通段场库内的火灾探测方案提供了参考。     

浅究路桥施工过渡段中施工技术问题

在路桥施工中整体流程中,过渡段中的施工是非常重要的,是保证路桥整体施工顺利进行的重要内容,并且,路桥施工中过渡段施工质量也会影响到路桥施工的综合质量。对于路桥施工过渡段中的施工技术问题的科学探讨,可以在一定程度上提升目前路桥施工中过渡段施工的质量,有助于我国路桥施工能力的发展和路桥施工质量的保证。本文从当前路桥过渡段施工的实际现状出发,对路桥施工中过渡段的施工技术相关问题进行了分析探讨,希望对相关的路桥施工单位有所启示。     

Effect of Nutrient Restriction and Compensation on Weight,Serum Indexes and Gene Expression of Chest Fat Adipokines in Mongolian Lamb

To study the effect of nutrient restriction and compensation on weight,serum indexes and gene expression of chest fat adipokine in Mongolian lamb,thirty male lambs were randomly divided into control group (20.26 kg± 3.63 kg) and feed restricted group (21.29 kg±3.00 kg).The experiment lasted for 120 days,including 60 days for feed restriction and 60 days for compensatory feed.At the end of each stage,five lambs from each group were slaughtered and chest fat was collected.Serum concentration of triglycerides,non-esterified fatty acids (NEFA),total cholesterol were measured.Quantitative Real-time PCR were used to determine Adiponectin,Visfatin,PPAR-γ and ACC genes expression.The results showed that:①The lamb body weight of restricted group kept maintain level and that of control group showed a increase tendency.During the compensatory period,the average daily gain (ADG) of restricted group was significantly higher than control group (P< 0.05).②During the restriction period,serum triglycerides concentration of lambs in restricted group was significantly decreased at day 30 and 60 (P< 0.05),and NEFA concentration was significantly increased at day 30 (P< 0.05).After 60 days of nutritional compensation,all serum parameters we tested were not significantly different between restrict group and control group (P >0.05).③During the restriction period,PPAR-γ and ACC gene expression in restricted group were significantly lower than that of control group (P< 0.05),while no significant differences were found during the compensation period (P>0.05).Adiponectin and Visfatin genes expression in chest fat was not significantly changed throughout the experiment (P >0.05).We could know that nutritional restriction reduced fatty acid synthesis and adipocyte differentiation ability,adipose tissue was mobilized energy metabolism,and total cholesterol was recoveried growth on the compensation.However,the ACC and PPAR-γ genes in restricted group were significantly decreased with the body weight increased rapidly during the compensatory stage.Adiponectin and Visfatin were not significantly different between restricted and compensatory stages.     

沙漠玫瑰

沙漠玫瑰（Adenium obesum）又名天宝花、小夹竹桃,原产非洲,为夹竹桃科天宝花属多年生肉质植物。株高可达2米,肉质根,茎粗壮,分枝多,叶色翠绿。如养护管理得当,春夏秋三季均可见花,是目前时尚的室内观赏化卉.