柑橘皮功能性物质种类及绿色联产工艺

随着柑橘产量的增加,从1990年代中期开始,我国柑橘已出现严重的相对过剩现象。参照国外柑橘业的发展史,我们不难发现柑橘业根本出路在于加工,只有加工,才能解决柑橘的出路,提高产品的附加值,才能提高国际市场的竞争力。     

cDNA Cloning and Sequence Analysis of Rice Sbel and Sbe3 Genes

Two starch-branching enzyme (SBE) in rice, is known to be a key enzyme in amylopectin biosynthesis. The cDNA of two SBE(starch-branching enzyme) genes SheI and Shed encoding SBE Ⅰ and SBE Ⅲ (two major isoforms in rice) were cloned by an improved RT-PCR technique, from a template cDNA libray, derived from the total mRNAs extracted from the immature seeds of a japonica rice Wuyunjing 7. DNA sequence analysis showed that the size of the cloned SheI and Shed cDNAs were 2490 and 2481 bp long, respectively, including their entire coding sequences. Comparison analysis indicated that the nucleotide sequence of She3 was the same as that of shed (Genbank Accession No. D16201) as reported previously. There were only four base-pairs difference,which resulted in changes of two deduced amino acids between the cloned She1 cDNA and the reported she1 (Genbank Accession No. D11082). The cloned SheI and Shed cDNAs make it possible to improve rice starch quality through genetic engineering.     

木聚糖酶产生菌的筛选及酶学性质研究

从实验室保藏的13株菌株中筛选到l株高产木聚糖酶菌株-黑曲霉2107,酶谱检测发现,该茵株产2种木聚糖酶X-1和X-2.X-1和X-2的最适反应温度都为50℃,并在30～50℃都较稳定.X-1的半失活温度50～55℃,X-2的半失活温度为55～60℃.X-1的最适pH约为5,pH 4～10较稳定;X-2的最适pH约为3和4之间,pH 2～5之间较稳定.各种金属离子对2种酶都有不同程度的抑制作用.     

蚕丝新用途研究进展

随着对蚕丝物理化学性质研究的深入，蚕丝新用途的研究得到较快的发展。本文在介绍了蚕丝新用途利用原理，介绍了蚕丝在化妆品，医药，固定化酶载体材料，食品以及再生丝和涂膜化纤表面等方面的应用和研究进展。     

耐高温酸性蛋白酶的菌株筛选及特性研究

本研究采用高温，超剂量常规诱变筛选方法，获得的耐高温酸性蛋白酶生产菌Ｓ３１５菌株，它所产生的耐高温酸性蛋白酶，最适ＰＨ值依次为２．５，３．０、２．０，最适温度为５０℃。     

Traits of physiological genetics related to photoinhibition of photosynthesis in rice

To elucidate genetic background of photoinhibition of photosynthesis (PS), we studied the changes of D_1-protein content and SOD activities in japonica and indica varieties, and their reciprocal hybrids after photoinhihition-inducing treatment by using isotope tracer. Judging from rate of photoinhibition (RPI) of PS, we found that japonica varieties were more tolerant to photoinhibition than indica varieties. How ever, the value of RPI of F_1 hybrids, though lying between those of their parents, was closer to that of their maternals than to that of paternals. Similar results were obtained in terms of PS Ⅱ activities and D_1-protein contents (Fig.1). To study the effects of SOD on PS in japonica and indica varieties. and their reciprocal hybrids,     

红星苹果和肥城桃果实软化的酶学基础

以红星苹果穴Malusdomesticacv.Starking雪和肥城桃穴Prunuspersicacv.Feicheng雪果实为试材,结合乙烯利和1-甲基环丙烯(1-MCP)处理,分别研究了2者软化的酶学基础,并就其差别作了探讨。结果表明:红星果实软化中起主要作用的酶为内切和外切多聚半乳糖醛酸酶(endo-和exo-PG);肥城桃果实软化的前20d则以纤维素酶(EGase)为主,20d后软化以endo-PG为主。研究同时发现,脂氧合酶(LOX)在这2种果实软化的启动阶段都起了明显的促进作用。1-MCP通过对红星果实中PG、肥城桃果实贮藏前期EGase和后期endo-PG活性的抑制,以及对2者软化启动阶段LOX活性的抑制,延缓了果实硬度的下降。