家鸽疟原虫一新种，蛛形疟原虫的记述（孢子虫纲：疟原虫科）

本文描述了广州地区家鸽之间流行的一新种疟原虫。因该虫形成蜘蛛形（Ｓｐｉｄｅｒｆｏｒｍ）滋养体阶段，故命名为蛛形疟原虫（Ｐｌａｓｍｏｄｉｕｍａｒａｃｈｎｉｄｉ，ｓｐ．ｎｏｖ．）。红细胞内发育成熟裂殖体最终产生６～１２个裂殖子及其包含丰富细胞质和长形配子体，无疑是隶属于齐粤虫亚属（Ｃｉｏ－ｖａｎｎｏｌａｉａ）。它的主要特征容易与近似种，即Ｇｕｉｎｄｙｅｔａｌ（１９６５）[３]在埃及戴胜鸟（Ｈｏｏｐｏｅ）记载的甘汉氏疟原虫（Ｐ．ｇａｒｎｈａｍｉ）相鉴别     

Preparation of Cathepsin L1 Monoclonal Antibody Against Fasclala gigantica and Establishment of Double Antibody Sandwich ELISA

【Objective】 This study was intend to obtain cathepsin L1(rFgCat L1) specific monoclonal antibody and construct the double antibody sandwich ELISA.【Method】 Five BALB/c mice were immunized with 1 mg/mL rFgCat L1 protein for four times.Mouse splenocytes were isolated and fused with SP2/0 cells to construct hybridoma cells.Strong positive hybridoma cell lines were screened, 1×10⁶ cells were injected intraperitoneally per mouse to prepare monoclonal antibodies.Antibody titer and antigenic epitope were detected using ELISA method, antibody subtype and specificity were identified using Western blotting method.The double antibody sandwich ELISA was constructed by combining the anti-rFgCat L1 polyclonal antibody, and its sensitivity and specificity were tested.The positive and negative critical value was screened by 20 negative sera with positive control, and the constructed double antibody sandwich ELISA was verified by 47 goat positive sera and 47 dairy cow positive sera.【Result】 After immunization, the antibody titers in serum of 4 mice were all more than 10⁴.After isolated mouse with the highest immune response spleen cells were fused with SP2/0 cells total of 8 of them were positive cell lines were obtained after selective culture.5D5 and 7G6 were identified as strong positive strains with stable antibody secretion.After multiple subcloning screens and subcultures, the antibodies secreted in the cell supernatant were stable, with titers of 2⁹ and 2¹⁰ respectively, with ascites titers of 10⁷ and 10⁸.Western blotting and antibody subtype identification kits identified that the two antibodies were IgG1 type and the light chain was kappa type, both of which could specifically bind FgESP.According to the same antigen site was recognized by the two kinds of antibodies, the antigen titer of the two monoclonal antibodies were comparied, 7G6 was used as the coating antibody, and anti-rFgCat L1 was used as the enzyme-labeled secondary antibody.The optimized condition of method was that 7G6 was coated at a concentration of 2 μg/mL, the dilution concentration of anti-rFgCat L1 polyclonal antibody was 25 μg/mL, the dilution of Don-HRP-conjugated was 1∶4 000, 5% skimmed milk powder was selected as the blocking solution and the color development time was 25 min.The method was proved that could recognize the lowest antigen concentration of 0.625 μg/mL, also could specifically recognize antigen of Fasciola fasciatus.The constructed sandwich ELISA method was used for antigen detection of 47 dairy cow positive serum and 47 goat positive serum infective samples kept in the laboratory and the positive antigen rate were 72.3% and 78.7%, respectively.【Conclusion】 Anti-rFgCat L1 monoclonal antibody was successfully prepared and the double-sheet sandwich ELISA method for fascioliasis was constructed, which provided a good theoretical basis and material basis for the development of low-cost and rapid diagnostic kits.     

兔体双腔吸虫调查和形态学研究

经对宁夏4个地区1085只家兔和35只野兔的调查,发现有3种双腔属（Dicrocoelium）吸虫:矛形双腔吸虫（D.lanceatum）、东方双腔吸虫（D.orientalis）和中华双腔吸虫（D.chinensis）。其中后2种为宿主新纪录。它们在家兔中的感染率依次为4.2％、1.8％、0.4％,在野兔中的感染率依次为14.3％、5.7％、5.7％。中华双腔吸虫呈纺锤形,体长5.702～6.934mm,体宽1.091～1.549mm,长宽比4.9:1,睾丸并列,阴茎囊椭圆形并达腹吸盘前缘,卵黄腺起自于睾丸之后、卵巢之前水平处;其余2虫种的形态与记载的基本一致。     

标准化肉牛育肥场建设

<正>1牛场选址标准化肉牛养殖场根据饲养规模可分为大、中、小三种类型,其存栏量分别为1000头以上、500~1000头、100~500头。牛场选址需综合考虑自然因素和社会因素。1.1地形与地势场址地形应整齐、开阔,方形最为理想,避免呈狭长形和多边形,应尽量利用荒地建场。牛场占地面积按15~25 m2/头估算,牛舍及其他房舍的面积占场地总面积的15%~20%。牛场应地势高燥,背风向阳,地下水位在2m以下,有缓坡,北高南低,总体平     

绵羊黑疫与肝片形吸虫混合感染的治疗

去年秋天,我市一些乡镇的养羊户反映,他们饲养的绵羊突然死亡、消瘦,剖检后发现肝脏坏死,当地人称“烂肝病”。其中有一户饲养绵羊150只,死亡21只,死亡率为14.0%;另一户饲养81只,死亡24只,死亡率为29.6%。     

牛羊肝片形吸虫病的治疗

肝片形吸虫病是牛羊最重要的寄生虫病之一。肝片形吸虫俗称柳叶虫,成虫寄生于牛羊等反刍动物的胆管中,引起肝实质炎,胆管炎和肝硬化等病变,表现为消化障碍,循环障碍和营养障碍等方面的症状,对牛羊健康危害极大。     

杀螨利器——伊虫宁

螨虫种类繁多,但影响宠物的主要是疥螨、蠕形螨和耳螨.疥螨寄生在宿主表皮角质层深处,以角质组织和淋巴液为食,并以螯肢和前跗爪挖掘,形成一条与皮肤平等的蜿蜒隧道.蠕形螨寄生于皮肤的毛囊、皮脂腺或体内淋巴结.耳螨会使耳道里的肥大细胞和巨噬细胞大量增加.螨虫的分泌物、代谢产物及死虫体会引起过敏反应,使皮肤瘙痒或导致皮肤病.     

酶切图谱及序列分析虫体ITS2鉴别中国的片形吸虫

提取我国广西、四川、黑龙江、甘肃等地及法国的片形吸虫的总DNA，用PCR扩增完整的ITS－2。对得到的PCR产物用限制性内切酶Hsp 92Ⅱ，RcaI进行酶切和RFLP技术分析。并对ITS－2PCR产物进行双向测序。以便确定国内片形吸虫种内及种间ITS－2的特点和序列变异的水平。结果显示：广西、四川、黑龙江、法国等地的样品通过PCR均扩增到约550bp的ITS－2目标片段。Hsp92 Ⅱ,Rcal可区别不同种的片形吸虫。对PCR产物的序列分析结果表明片形吸虫ITS－2全长均为362bp，同种内ITS－2序列无变异，不同种间ITS－2序列存在5－6个碱基差异，变异率约为2.8%。对各地区片形吸虫ITS－2的PCR－RFLP分析与对PCR产物的DNA序列分析一致证明，来自四川的样品和法国样品同属肝片形吸虫F.hepatica；广西样品属大片形吸虫F.gigantica；黑龙江的样品为中间型片形吸虫。本研究首次从分子水平上证实我国除有肝片形吸虫、大片形吸虫外，还存在中间型的片形吸虫。     

治疗牛乳房炎的新器械--黄瓜形乳头管

当前在治疗母牛乳房炎、乳房创伤、乳房蜂窝织炎、乳房疖肿、母牛产后轻瘫等病，还仍在使用老式器械——奥杰斯脉冲导管。而今我们介绍的黄瓜形乳头导管，治疗效果要比奥杰斯脉冲导管好得多。