Development of Nucleic Acid Lateral Flow Immunoassay Detection Method for Foot-and-mouth Disease Virus

In this article, through the combination of nucleic acid probes and immune chromatography, a simple, sensitive and specific detection system——nucleic acid lateral flow immunoassay (NALFIA) for amplifing foot-and-mouth disease virus (FMDV) 3D RT-PCR products was established.An ultrasensitive nucleic acid biosensor (NAB) based on streptavidin-labeled gold nanoparticles dual labels and lateral flow strip biosensor (LFSB) were used in this system.The biotinylated goat anti-rabbit IgG was marked to the NC membrane as the alleged strip and the anti-digoxin antibody was labeled to the NC membrane to capture the digoxin probe.After assemblying gold-labeled strip and detecting RT-PCR products, the detection limit of NALFIA was 0.3×10^-3 to 3×10^-3 μg/μL.The NALFIA was compared with agar gel electrophoresis analysis, the results showed that the sensitivity of NALFIA was higher than agar gel electrophoresis.There was an excellent agreement between the two methods.NALFIA was a method with high sensitive, low cost and short time.In conclusion, this method provided a good alternative to detect FMDV.     

玉米叶片衰老相关基因的研究进展

玉米叶片衰老对子粒形成和产量有着重要的影响。大量研究表明,叶片衰老受众多因素影响,造成形态及生理生化的巨大变化,而这些变化均离不开基因的调控作用。本文综述了近年来国内外有关玉米叶片衰老的研究结果,主要包括玉米叶片衰老的生理生化和栽培技术研究及有关玉米叶片衰老的分子生物学研究,以期能为玉米叶片衰老的进一步研究提供参考。     

抗条纹叶枯病基因STV11的功能性分子标记建立及粳型抗源筛选

条纹叶枯病是水稻(Oryza sativa L.)上最严重的病害之一。STV11是从籼稻Kasalath中鉴定的条纹叶枯病抗性基因,根据Kasalath型条纹叶枯病基因STV11 KAS6个碱基缺失的功能性多态性序列差异,设计基于PCR的功能性分子标记qstv11。利用此标记对299份试验材料[5份抗病品种、5份感病品种、41份辽宁省育种材料、17份日本粳型材料、142份秋光/七山占重组自交系(群体A)、85份笹锦/Habataki//笹锦回交重组自交系(群体B)、群体亲本4份]进行基因型分析并进行抗病性鉴定。结果表明,qstv11能够较好区分抗感基因型,而且在测试材料中与条纹叶枯抗性整体呈现共分离。41份辽宁材料中鉴定出含有STV11-R(抗)材料1份;日本粳型材料该位点均为STV11-S(感);群体A和B中分别筛选出粳稻背景STV11-R型材料24份和6份。     

蜂花粉对公鸡繁殖性能的影响

<正>伴随着国家经济的快速发展,人民的生活水平也日益增高,对于食品的质量要求也相应增加,喂养全过程的安全性更要保证到位,即饲料的安全性、成长环境的清洁型。因此,饲料的配方在很大程度上决定了动物的质量。近些年,蜂花粉以其营养元素全面、环保的特性备受欢迎。蜜蜂采蜜时带回的花粉团,经蜂巢内部蜂群的特殊处理、储藏和发酵后形成的花粉称作蜂花粉。蜂花粉是有花植物雄蕊中的雄性     

Infections Investigation of ALV and REV in Guangxi Local Poultry Breeds and Sequence Analysis of gp85 Gene of ALV Isolates

In order to investigate the infection status of avian leukosis virus (ALV) and avian reticuloendotheliosis virus (REV) in the major local breeds of Qinzhou,Guangxi,totally 953 samples of egg white,cloaca swab and serum of Ma duck,Shitou goose,Tiejiao-Ma chicken,turkey and pigeon were collected from the representing flocks and detected by the commercial ELISA kits.ALV was isolated for the ALV p27 positive samples by culturing on DF-1 cells,and gp85 gene was sequenced.The results showed that the detections of ALV were negative in the samples except those of Tiejiao-Ma chicken,while REV antibody was found positive in Ma duck,Tiejiao-Ma chicken and turkey.The nucleotide sequences of gp85 gene of two isolates shared 94.5% identity with each other,and shared 86.9% to 94.9% with reference strains.The amino acid sequences of gp85 gene of two isolates shared 91.5% identity with each other,and shared 84.0% to 91.6% with reference strains.There were many variable sites in the hyper variable region hr1 and hr2,and the vr2 and vr3 variable regions were relatively conservative.Phylogenetic tree analysis showed that the two isolates shared the highest homology with SCAU11-XG strain.     

箱型柱混凝土顶升浇筑技术措施

在建筑工程的施工建设过程中,由于箱型柱混凝土提升浇筑技术性高效性的特点.以及对建筑结构稳定性的加强作用,箱柱型混凝土成为最常用的技术手段.在本文中笔者就箱柱型混凝土顶升浇筑技术中可能出现的问题进行阐述,并对其解决措施进行分析,希望可以给予相关技术人员借鉴与帮助.     

Analysis on Target Genes with Mutation in microRNA of Blue-shelled Chicken and White Leghorn

The study was conducted to explore the potential different characters between Blue-shelled chicken and White leghorn.Global genome microRNA was combined the identified microRNA with complementary lab-predicted microRNA.Then the two breed chicken's SNP data got by GGRS were mapped to the microRNA and focused on SNP that deliberately located in mature-microRNA.Bioinformatics method was adopted for target prediction on microRNA which had SNPs.By further gene enrichment analysis,the study found these genes enriched in 22 GO terms,10 KEGG pathways,and 3 IPA important networks.And they enriched in traits which associated with growth,such as mTOR signaling pathways,Wnt signaling pathways,growth hormone receptor networks and insulin-like growth factor Ⅰ receptor networks.And they also enriched in some laying traits,such as oocyte meiotic signaling pathways and progesterone mature oocytes signaling pathways.The methods and the results might provide references for further studies.     

RBP4基因与动物繁殖性能的相关性

视黄醇结合蛋白4(RBP4)是动物体内一种重要的转运蛋白,它在介导维生素A及其代谢物生理功能的正常发挥中起着不可替代的作用。RBP4是孕体产生的主要蛋白之一,对动物的胚胎发育和繁殖性能有较显著的影响。RBP4的功能障碍会导致维生素A的储存、转运、分布及代谢的异常,进而引发各种疾病,并影响上皮、骨组织的生长、分化与繁殖、胚胎发育。本文综述了RBP4基因的结构与定位,RBP4的生物学功能,以及RBP4基因与动物繁殖性能的关系。     

细胞自噬的研究进展

自噬(autophagy)是广泛存在于真核细胞中的细胞生物学行为,是一种溶酶体依赖性降解途径,是细胞进行自我保护的一种重要机制,对于维持细胞存活、更新、物质再利用和内环境稳定中起着重要作用。近年来,诸多的研究表明,细胞自噬积极参与多种病原体感染宿主动物/细胞的过程,而且在不同病原体的感染过程中所起的作用不尽相同。本文从自噬的发现及分类、发生过程、自噬调控基因、检测方法和自噬与病原体相互关系进行了综述。