Research Progress on Action Mechanism of Animal Anti-parasitical Drugs

Animal anti-parasitical drugs are mainly used to prevent and control animal parasitosis, and also an effective way to protect the healthy development of animal husbandry and public health security.The efficacy of anti-parasitical drugs depends on the interaction between drug molecular and different target tissues and target cells of parasite or animal.Because of the variety of anti-parasitical drugs, the anti-parasitical mechanisms are complex.With the development of new anti-parasitical drugs and the depth of scientific research, especially the development of chemical synthesis and biological pharmaceutical technology, the variety and quantity of anti-parasitical drugs constantly increase.With the discovery of new drug structure and its action target, the study on the mechanism is constantly deep.The author mainly summarized the research progress on action mechanism of anti-parasitical drugs.     

动物诊疗机构人用药品使用现象分析及建议

随着社会经济水平日益提升,越来越多的人饲养宠物作为伴侣,与此同时宠物诊疗机构如春笋般蓬勃发展。然而其在发展中存在诸多问题,尤以使用人用药品问题最为突出。论文从药品使用现状、原因、管理等方面,对动物诊疗机构人用药品使用现象进行分析并提出一些建议,旨在进一步规范动物诊疗机构临床用药,使其良性发展。     

Surgical treatment of mammary carcinomas in dogs with or without postoperative chemotherapy

This retrospective study identified prognostic factors associated with survival; and compared survival data in 94 canine mammary carcinoma (MCA) dogs treated with surgery (n = 58), or surgery and adjunct chemotherapy (n = 36), and a subset of dogs with poor prognostic factors. On multivariate analysis independent predictors of median survival time (MST) were clinical stage, lymphatic invasion (LI; present 179 days; none 1098 days), ulceration (present 118 days; none 443 days) and surgical margins (incomplete 70 days; complete 872 days). Complete surgical margins were associated with MST in dogs with stages 1–3 MCA (incomplete 68 days; complete 1098 days) and dogs with LI (incomplete 70 days; complete 347 days). There was no statistically significant improvement in MST in dogs with advanced disease (stage 4 or LI) treated with adjunctive chemotherapy (chemotherapy 228 days; none 194 days); although five dogs with complete surgical margins that received mitoxantrone and carboplatin had a mean survival of 1139 days.     

Evaluation of truncated LipL32 expressed by Escherichia coli and Pichia pastoris for serodiagnosis of Leptospira infection in rodents

The applicability of the recombinant LipL32 for serodiagnosis of leptospiral infection in field rodents was assessed in this study. An immunodominant region of LipL32 was determined by monoclonal antibodies, and then, truncated LipL32 (tLipL32) was designed to contain the region (87–188th amino acid). The tLipL32 was compared between two recombinant expression hosts Escherichia coli and Pichia pastoris in ELISA. With field rat sera, tLipL32 expressed by P. pastoris (tLipL32p) had high antigenicity without background reactions, while tLipL32 expressed by E. coli (tLipL32e) showed high background reactions, which were reduced by pre-adsorption of sera with E. coli. To evaluate tLipL32-ELISA, field rat sera were tentatively divided into a Leptospira infection positive (12 sera) and a negative group (12 sera) based on the results from flaB gene PCR of kidney samples and WB with whole Leptospira cell. Consequently, the sensitivity of tLipL32p-ELISA for field rat sera was 83% . A similar result was obtained from tLipL32e-ELISA with adsorbed sera, (92%). However, sensitivity of tLipL32e-ELISA using sera without an adsorption treatment was 50%. Regardless of the expression host, tLipL32-ELISA had 100% specificity and sensitivity in experimentally infected laboratory rats. These results suggest that recombinant LipL32 expressed by P. pastoris is more applicable for serodiagnosis in field rats due to a lack of background reaction.     

Neuronal ceroid lipofuscinosis: clinical and morphologic findings in nine affected Polish Owczarek Nizinny (PON) dogs

OBJECTIVE: The aim of this study was to characterize the clinical and morphologic features of neuronal ceroid lipofuscinosis (NCL) in the Polish Owczarek Nizinny (PON) breed of dog. ANIMALS: Nine Swedish PON dogs of both sexes were included in the study. PROCEDURE: All dogs underwent a detailed clinical evaluation, with emphasis on ophthalmic exams. Histopathology and electron microscopy were performed on the eyes, brain and various internal organs. Immunohistochemical staining for detection of sphingolipid activator proteins (SAPs) and mitochondrial ATP synthase (SCMAS) was performed on the eyes and brain. RESULTS: The dogs showed behavioral abnormalities, motor disturbances and visual impairment or blindness. Pupillary responses were abnormal while fundus changes varied from normal to severe retinal atrophy. Electroretinography (ERG) showed variable changes, from slight alterations in the process of dark adaptation to severely reduced or nonrecordable ERG a- and b-wave amplitudes. Histopathology revealed intracytoplasmic storage bodies within neurons of the brain and in retinal cells, especially the retinal pigment epithelium (RPE). Round to oval granular type of inclusion bodies, known as granular osmiophilic dense deposits (GRODS), were found in neuronal cells in the brain and in the retina. Immunohistochemistry identified the storage material in the brain and retina as consisting of SAPs. CONCLUSION: The presently described NCL disease in PON dogs shows similarities to previously recorded cases in the Miniature Schnauzer. The closest human equivalent to this disease is infantile NCL (CLN1), in which the major stored proteins are SAPs and the ultrastructure of the inclusion bodies of neuronal cells is granular.     

中美动物源微生物耐药性监测对比研究

抗微生物药物在保障动物健康和食品安全方面发挥了重要作用,为了应对日益严峻的微生物耐药性问题,动物源微生物耐药性监测是应对微生物耐药性风险的重要防控策略。在“同一健康体系”工作框架下,中国和美国积极进行动物源微生物耐药性监测。本文通过对比研究中美动物源微生物耐药性系统的监测设计、监测对象和监测数据应用情况,结合我国畜牧兽医行业现状,立足我国动物源微生物耐药性监测客观需求,旨在为我国动物源微生物耐药性监测工作提供参考。     

Some Ideas on Reconstruction of Villages inside the City

In this paper, the historical reasons of formation of the village inside the city are analyzed. It is indicated that such situation for the villages inside the city are the result of joint action of both the villagers and the government. The foundation of villages inside the city laid on the simple compensation for farmers in confiscating the rural land and lack of intelligence aids. In this paper, the ideas and measures for their reconstruction are put forward based on consideration of the reasons of their formation and difficulties in the reconstruction.     

Selecting Bid with Reasonably Low Price Without Bottom Price Based On Sector Estimate

For raveling out the main shortage of tendering which is the reasonable sector is unreasonable of making the bid evaluation uncertain. In order to eliminate subjective factor, this paper quantificationally analyses the price in bidding and establishes the sector selecting bid model based on the sector estimate idea, which tries to provide a new method and academic basic for determining the reasonable sector. Finally, the article gives an example to prove that the model is feasible and effective and it tries to ensure the bidding assessment public, equitable and just.     

PROBABILISTIC EVALUATION FOR STEEL FRAME COLUMNS OF TALL BUILDINGS

Based on properties of parameters and test data of dom-estic steel,this paper analyses the reliability and partial coefficient forresistance of steel frame columns of tall builings.The result may serveas a scientific basis.It is not only for domestic steel instead of importedsteel in building tall steel structures,but also for compiling concerendstandards.     

The Software System for Computation and Drawing of Ship Properties

The softwa re system suggested in this paper is applied to replace the traditionalhanded-computation forms. It uses the cubic sample interpolation and numerical integral,and is writ-ten with FORTRAN according to the accurate formula. With this software system,the nine sorts ofcomputation results of ship properties can be obtained. The system is con nected through SCR filewith AutoCAD,and ean draw automatically eleven sorts of pictures according to the computation re-sults.