动物源性食品中小分子药物残留的酶联免疫学检测研究进展

基于酶联免疫学检测原理及分类,阐述了酶联免疫学测定方法的建立,包括半抗原的设计及合成,人工免疫全抗原的合成,特异性抗体的制备等方面,并论述了兽药残留免疫检测技术的应用进展.     

HPLC法测定兽用中药散剂中非法添加利巴韦林方法研究

建立高效液相色谱法检测白龙散、黄连解毒散、苍术香连散等7种兽用中药散剂中非法添加利巴韦林的方法。用十八烷基硅烷键合硅胶色谱柱,以水(用稀硫酸调节pH值至2.5±0.1)为流动相,检测波长207 nm,流速1.0 mL/min,进样量20μL。结果显示,利巴韦林的线性范围为1.016-20.32μg/mL,线性方程为Y=5.68×10 X-5.61×10-(2r=0.999 9,n=6),回收率为81.28%～119.46%,RSD为0.88%～1.65%。本方法准确可靠,适用于兽用中药散剂中非法添加利巴韦林的检测。     

Magnetic resonance imaging metallic artifact of commonly encountered surgical implants and foreign material

Magnetic resonance imaging (MRI) artifacts secondary to metallic implants and foreign bodies are well described. Herein, we provide quantitative data from veterinary implants including total hip arthroplasty implants, cranial cruciate repair implants, surgical screws, a skin staple, ligation clips, an identification microchip, ameroid constrictor, and potential foreign bodies including air gun and BB projectiles and a sewing needle. The objects were scanned in a gelatin phantom with plastic grid using standardized T2-weighted turbo-spin echo (TSE), T1-weighted spin echo, and T2*-weighted gradient recalled echo (GRE) image acquisitions at 1.5 T. Maximum linear dimensions and areas of signal voiding and grid distortion were calculated using a DICOM workstation for each sequence and object. Artifact severity was similar between the T2-weighted TSE and T1-weighted images, while the T2*-weighted images were most susceptible to artifact. Metal type influenced artifact size with the largest artifacts arising from steel objects followed by surgical stainless steel, titanium, and lead. For animals with metallic surgical implants or foreign bodies, the quantification of the artifact size will help guide clinicians on the viability of MRI.     

我国兽医药学科近年研究与应用进展(Ⅰ)

兽药(含饲料药剂)是实现现代养殖的物质基础。本文通过2009与2011年全国10次和11次兽医药理毒理学术研讨会的论文[1,2]综述,介绍兽医药学科技近年研究与应用进展,分为:重要主题,基础药理与临床治疗学,兽药代谢动力学与药物残留,抗菌药与细菌耐药性,中草药及化药新剂型新制剂的研发等5部分。     

对我国兽药行业官方评议工作的思考

从官方评议工作背景、工作成效、存在问题及工作建议四方面对我国兽药行业官方评议工作进行介绍、分析和思考,并以案例分析阐述如何提升评议质量。     

兽药耐药性情况分析及防控建议

全面论述了耐药性危害,分析了耐药性产生原因和现状,从宏观管理和临床控制两方面指出应对措施,并结合国外耐药性监测情况提出开发全新抗菌药和改造已产生耐药性的抗菌药、开发不使用抗菌药物治疗感染的治疗策略以及建立耐药性监察系统的发展趋势.     

OIE抗菌药耐药性国际标准

兽用抗菌药耐药性已经成为一个全球普遍关注的公共健康问题,各国际组织都积极采取相应的措施控制耐药性的产生和蔓延。介绍了国际组织世界动物卫生组织OIE制定的五个国际标准,包括协调抗菌药耐药性监督和检测程序指南、畜牧业抗菌药消耗量监测指南、兽用抗菌药慎用指南、抗菌药敏感性检测的实验室方法指南、动物源抗菌药耐药性对公共健康潜在影响的风险分析方法指南,以期为我国政策制定者和决策者参照国际标准制定出符合我国国情的耐药性相关指南。     

SCI收录的兽医学科主要期刊评价与投稿策略

SCI是国际上权威的自然科学基础研究成果评价体系,利用2010年度期刊引证报告（JCR2010）数据．分析了SCI兽医学科源期刊评价指标,根据引文分析方法筛选了兽医学科中较具影响力的期刊．并给出了投稿建议。     

应用青海弧菌评价常见8种兽药的急性毒性

以青海弧菌Q67(Vibrio qinghaiensis sp-Q67)为毒性测试发光微生物,使用BHP9511型水质毒性快速检测仪,通过测定其在8种常见兽药不同质量浓度下的相对发光率和8种兽药对其半数抑制效应质量浓度(EC50),研究8种兽药对青海弧菌的作用规律及急性毒性效应。结果表明,在所研究的质量浓度范围内,8种兽药对青海弧菌的相对发光率随兽药质量浓度的降低而增加;暴露时间为15min时,8种兽药对青海弧菌的急性毒性大小依次为呋喃唑酮>左氧氟沙星>诺氟沙星>磺胺六甲氧嘧啶钠>沙拉沙星>恩诺沙星>环丙沙星>磺胺间甲氧嘧啶纳。     

A longitudinal study of veterinary students and recent graduates 4. Gender issues

Objective To study differences in experiences, attitudes and opinions between female and male veterinary students and recent graduates.
Design Longitudinal study.
Population Students – 77 males and 77 females – who began studying veterinary science at The University of Queensland in 1985 and 1986.
Procedure Questionnaires were completed in the first and fifth year of the course, and in the second year after graduation. The data were analysed using the SAS System for Windows.
Results Females decided to study veterinary science at a younger age than males and were more influenced by 'a love of animals', the image of veterinarians as portrayed on television, an interest in living things and in the scientific study of disease. Males were more influenced than females in aspects of the workplace: bosses and money. There were no gender differences in their background in relation to farms, or to animals, or in their career plans. However females when in first year expected a lower initial income than males; an expectation that was realised in the first year after graduation. As first year students and also as veterinarians, females had stronger views than males on animal welfare issues, and also felt that the veterinary profession had a lower status relative to other professions.
Conclusions Significant differences in attitudes and experiences exist between males and females entering the veterinary profession. The situation of females in relation to income and status is consistent with that in other professions, where females have been disadvantaged compared with males.