Human gene therapy vectors derived from feline lentiviruses

Lentiviral vectors are useful for gene transfer to dividing and nondividing cells. Feline immunodeficiency virus (FIV) vectors transduce most human cell types with good efficiency and may have advantages for clinical gene therapy applications. This article reviews significant progress in the development and refinement of FIV vector systems.     

乙肝疫苗植物表达载体的构建

将乙肝病毒表面抗原(HBsAg)基因构建到植物表达载体pBI121的单子叶植物特异启动子UΩ下游,通过酶切和PCR检测分析鉴定证实重组质粒插入正确。      

嗜热菌穿梭质粒的研究进展

综述了嗜热菌基因工程及遗传转化系统的研究现状,介绍了现有几种已经成功转化的嗜热菌以及应用于它们的遗传转化系统中的穿梭质粒,分析了它们各自的优缺点及在嗜热菌基因改造中的应用前景。     

Maize streak virus: evidence for resistance againstCicadulina mbila Naude,the main vector species

Summary Settling, probing and oviposition behaviour ofCicadulina mbila, the main vector of maize streak virus (MSV) disease were studied on four maize genotypes, 100MSR, HASR, Reunion and H512. Of the four, only H512 was completely susceptible to MSV while the other three genotypes were all known to have some resistance to MSV. Generally,C. mbila settled in higher numbers on the MSV susceptible genotype compared to the three virus resistant ones. However, the number of probing marks left on H512 and 100MSR were similar while more marks were made on Reunion and HASR in increasing order. The oviposition patterns were similar to those observed for probing behaviour. The results suggest the existence of potentially useful resistance mechanisms against the vector. The possible implications of these results are discussed.     

植物病毒-新型的外源基因表达载体

与利用转基因植物表达外源基因相比,植物病毒载体具有表达水平高、速度快、宿主广等优点,是一类新型的外源基因表达载体.本文就植物病毒表达载体的构建策略、应用状况和存在的问题进行了综述.     

苹果MxNas1基因正反义表达载体构建及转化烟草的研究

为研究笔者前期克隆的MxNas1基因功能,构建了苹果属植物小金海棠MxNas1基因正义和反义两种表达载体,并用农杆菌介导的叶盘转化法转化了烟草.对转基因烟草分别进行0和1μmol/L Fe处理14d后,转正义载体烟草在缺Fe情况下叶片没有黄化,表现出较强的抗缺Fe能力;转反义载体植株比对照烟草幼叶提前出现黄化现象,表现...     

对数据图示化的研究

本文结合传热、流体流动问题探讨了将所得数据转换成等值线和速度矢量线的方法及作图的计算程序。这个方法已被运用到解释复杂的流动现象并得到了良好的效果。这个方法的基本原则也能推广到用计算机数值模拟和计算机控制的测试采样所得到的信息或数据处理中去,作为解释物理现象的一种有效手段。     

小麦AGPase胞质型基因LSUI的克隆及正义、反义与RNAi干扰载体的构建

采用RT-PCR方法从小麦品种豫教2号的发育籽粒中克隆出AGPase胞质型大亚基基因(large subunit,LSU I)的cDNA全长(1947 bp,GenBank No.DQ839506),同源性比较结果表明,与GenBank上已报道的LSU I基因同源性达99%,虽与其有2处碱基不同(与Z21969相比,分别在851 bp处的T变为C和926 bp处的G变为A),但他们的氨基酸序列相同,故不影响其功能.以pBI121质粒为基础,通过中间载体pBSK,构建了由35S启动子调控的LSU I基因的正义表达载体pBI121LSUⅠS;将该基因反向置于pBI121质粒的CaMV35S启动子之后,构建了LSU I的反义表达载体pBI121LSUⅠA.同时还克隆出LSU I基因的250 bp片段,以pFGC5941质粒为基础,构建了RNAi干扰载体pFGCLsuⅠ,这为研究该基因的功能打下了很好的基础.     

New Invertebrate Vectors for PST,Spirolides and Okadaic Acid in the North Atlantic

The prevalence of poisoning events due to harmful algal blooms (HABs) has declined during the last two decades through monitoring programs and legislation, implemented mainly for bivalves. However, new toxin vectors and emergent toxins pose a challenge to public health. Several locations on the Portuguese coast were surveyed between 2009 and 2010 for three distinct biotoxin groups [saxitoxin (PST), spirolide (SPX) and okadaic acid (OA)], in 14 benthic species of mollusks and echinoderms. Our main goals were to detect new vectors and unravel the seasonal and geographical patterns of these toxins. PSTs were analyzed by the Lawrence method, SPXs by LC-MS/MS, and OA by LC-MS/MS and UPLC-MS/MS. We report 16 new vectors for these toxins in the North Atlantic. There were differences in toxin contents among species, but no significant geographical or seasonal patterns were found. Our results suggest that legislation should be adjusted to extend the monitoring of marine toxins to a wider range of species besides edible bivalves.     

桉树和相思树Ceratocystis枝干枯萎病研究进展

Ceratocystis致病菌可感染树木枝干和根部的伤口部位,导致被感染部位枯萎,严重情况下可导致枝干或整株树木死亡。桉树和相思树是世界热带、亚热带地区的重要商品林树种,Ceratocystis枝干枯萎病给桉树和相思树的健康发展带来了一定的威胁,特别是在东南亚和非洲地区。最近,本文作者从广东湛江地区的桉树商品林里面分离并鉴定到两个Ceratocystis种,Cacaciivora和一个新描述的种Cchineucensis,致病力测试显示Cacaciivora对测试的桉树无性系具有较强的致病力,桉树不同无性系对Cacaciivora的抗性存在显著的差异。本文介绍了Ceratocystis的生物学特性、分类依据和方法,在世界范围内的分布和危害以及防控措施,以应对Ceratocystis病原对我国华南桉树和相思树商品林的危害。