全文获取类型
收费全文 | 164篇 |
免费 | 32篇 |
国内免费 | 6篇 |
专业分类
林业 | 5篇 |
农学 | 3篇 |
1篇 | |
综合类 | 28篇 |
农作物 | 1篇 |
水产渔业 | 25篇 |
畜牧兽医 | 131篇 |
园艺 | 8篇 |
出版年
2024年 | 2篇 |
2023年 | 8篇 |
2022年 | 3篇 |
2021年 | 12篇 |
2020年 | 7篇 |
2019年 | 18篇 |
2018年 | 7篇 |
2017年 | 13篇 |
2016年 | 7篇 |
2015年 | 9篇 |
2014年 | 7篇 |
2013年 | 13篇 |
2012年 | 13篇 |
2011年 | 8篇 |
2010年 | 9篇 |
2009年 | 16篇 |
2008年 | 5篇 |
2007年 | 6篇 |
2006年 | 6篇 |
2005年 | 5篇 |
2004年 | 2篇 |
2003年 | 4篇 |
2002年 | 2篇 |
2001年 | 3篇 |
2000年 | 3篇 |
1999年 | 2篇 |
1997年 | 4篇 |
1996年 | 2篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有202条查询结果,搜索用时 15 毫秒
141.
Ulcerative facial and nasal dermatitis and stomatitis in cats associated with feline herpesvirus 1 总被引:1,自引:0,他引:1
Ulcerative dermatitis of the nasal planum or haired skin of the face, associated with intranuclear inclusion bodies compatible with herpesvirus, was identified in nine cats. Clinically, lesions were ulcerative and crusted, and often persistent. A tenth cat had focal proliferative ulcerative stomatitis, also associated with intranuclear inclusion bodies. Microscopically, there was necrosis and ulceration associated with prominent eosinophilic inflammation. Intranuclear inclusion bodies were noted in all cases, within the surface or adnexal epithelium. Ultrastructural examination of skin from two cats revealed virions morphologically compatible with a herpesvirus. Polymerase chain reaction (PCR) specific for feline herpesvirus 1 on DNA extracted from fresh-frozen or formalin-fixed paraffin-embedded biopsy samples and/or consensus primer PCR with DNA sequencing performed on DNA extracted from formalin-fixed paraffin-embedded biopsy samples from seven cats revealed that the virus was indistinguishable from feline herpesvirus 1. PCR was negative in one of eight cats tested. 相似文献
142.
143.
网箱养殖大黄鱼溃疡病的预报模型 总被引:2,自引:1,他引:1
本文以舟山市网箱养殖大黄鱼Pseudosciaena crocea (Richardson)为研究对象,根据对2001-2005年间网箱养殖大黄鱼发病情况的观察分析、养殖水域环境因子的监测及其收集的气象资料,对大黄鱼溃疡病的发生规律和养殖环境的状况进行了全面分析,结果表明大黄鱼溃疡病的发生不仅与季节有关,而且还与环境因子的状况密切相关。在此基础上,进一步研究了环境因子对大黄鱼溃疡病发生的影响程度和影响方式,筛选出与大黄鱼溃疡病发生密切相关的环境因子,从而建立了网箱养殖大黄鱼溃疡病发生的预报模型。经检验该模型对预报大黄鱼的溃疡病是否发生及发生的程度有高度显著的效果,用该模型对舟山市2005年大黄鱼溃疡病的发生情况进行了预报,预报的正确率达到81.2%。为能有效、适时地对发病季节大黄鱼疾病的控制,本文还同时给出了网箱养殖大黄鱼发病季节溃疡病的预报模型。 相似文献
144.
以养殖刺参(Apostichopus japonicus)腐皮综合征的2种致病菌:灿烂弧菌(Vibrio splendidus)和假交替单胞菌(Pseudoalteromonas nigrifaciens)为抗原,分别制备兔抗血清。以载玻片为介质,建立了2种病原菌的间接荧光抗体检测技术(IFAT)。交叉反应、阻断试验和吸收试验结果均表明本方法特异性强。对人工感染实验中的养殖水体及发病刺参溃烂组织检测,可以检出水体和患病刺参溃烂组织中的相应病原菌,病原菌被染成明亮的黄绿色,检测灵敏度2.4×104cell/mL。冰冻切片检测结果显示,在刺参肿胀嘴部与溃烂肌肉处有大量染成黄绿色的细菌颗粒。结果表明:采用IFAT可以对刺参腐皮综合征2种致病菌进行准确快速的检测。该方法的建立对刺参腐皮综合征的流行病学调查及快速诊断具有重要意义。 相似文献
145.
Outbreaks of ulcerative disease associated with ranavirus infection in barcoo grunter,Scortum barcoo (McCulloch & Waite) 下载免费PDF全文
P Kayansamruaj A Rangsichol H T Dong C Rodkhum M Maita T Katagiri N Pirarat 《Journal of fish diseases》2017,40(10):1341-1350
In 2013, an outbreak of ulcerative disease associated with ranavirus infection occurred in barcoo grunter, Scortum barcoo (McCulloch & Waite), farms in Thailand. Affected fish exhibited extensive haemorrhage and ulceration on skin and muscle. Microscopically, the widespread haemorrhagic ulceration and necrosis were noted in gill, spleen and kidney with the presence of intracytoplasmic eosinophilic inclusion bodies. When healthy barcoo grunter were experimentally challenged via intraperitoneal and oral modes with homogenized tissue of naturally infected fish, gross and microscopic lesions occurred with a cumulative mortality of 70–90%. Both naturally and experimentally infected fish yielded positive results to the ranavirus‐specific PCR. The full‐length nucleotide sequences of major capsid protein gene of ranaviral isolates were similar to largemouth bass virus (LMBV) and identical to largemouth bass ulcerative syndrome virus (LBUSV), previously reported in farmed largemouth bass (Micropterus salmoides L.), which also produced lethal ulcerative skin lesions. To the best of our knowledge, this is the first report of a LMBV‐like infection associated with skin lesions in barcoo grunter, adding to the known examples of ranavirus infection associated with skin ulceration in fish. 相似文献
146.
Vilar P Faílde LD Bermúdez R Vigliano F Riaza A Silva R Santos Y Quiroga MI 《Journal of fish diseases》2012,35(6):437-445
This study describes morphopathologic changes in naturally infected farmed Senegalese sole affected by tenacibaculosis caused by Tenacibaculum maritimum. Macroscopic observation, in addition to light microscopy and scanning electron microscopy, was used to study the lesions. Main lesions were characterized by complete loss of epidermis and dermis, as well as extensive necrosis of muscle layers. Mild-to-moderate inflammatory response with the presence of macrophages was noted around hyaline degenerated muscle cells. Gram-negative filamentous bacteria could be detected only at the dermis. Under scanning electron microscopy, filamentous bacteria located over the scales without epithelium could be observed. These findings together with the isolation and PCR detection of the bacteria in kidney and skin tissues suggest that once the bacteria reach the dermis, probably through eroded epidermis, they are able to proliferate and produce enzymes that are responsible for the damage in the underlying tissues. 相似文献
147.
Carlos Areche Julio Benites Alberto Cornejo Lina M. Ruiz Olimpo García-Beltrán Mario J. Simirgiotis Beatriz Sepúlveda 《Marine drugs》2015,13(4):1726-1738
Ten known meroterpenoids and the new meroterpenoid 7 were isolated from the Chilean seaweed Stypopodium flabelliforme as their acetylated derivatives. Furthermore, the known metabolite taondiol has been isolated for the first time from this species. The molecular structure of the new metabolite was determined by spectroscopic methods based on 1D- and 2D-NMR. Isolation of 7 represents a key step toward a better understanding of the biogenesis of this class of meroterpenoids. Among the meroditerpenoids isolated, stypodiol, isoepitaondiol, epitaondiol and sargaol exhibited gastroprotective activity on the HCl/Ethanol-induced gastric lesions model in mice. Regarding the mode of gastroprotective action, the activity of epitaondiol was reversed significantly when animals were pretreated with indomethacin, N-ethylmaleimide and N-nitro-l-arginine methyl ester (L-NAME) suggesting that prostaglandins, sulfhydryl groups and nitric oxide are involved in their mode of gastroprotective action. In the case of sargaol the gastroprotective activity was attenuated with indomethacin and N-ethylmaleimide, which suggests that prostaglandins and sulfhydryl groups are also involved in the mode of action using this model. 相似文献
148.
猪胃溃疡的发生机制及酸化剂对其的影响 《畜牧与饲料科学》2021,42(5):39-44
受传统“无酸无溃疡”观念的影响,在酸化剂普遍应用于饲料端和养殖端的今天,人们较为关注酸化剂是否影响猪胃的健康、是否促进了胃溃疡的发生。猪胃不同区域的功能存在差异,胃溃疡发生的频率和机理明显不同。从胃黏膜的结构、胃酸、胃蛋白酶和胆汁等方面阐述了猪胃溃疡的发生机制,并对酸化剂是否加剧胃溃疡的发生进行综述,以期为酸化剂的合理应用提供参考。 相似文献
149.
齐口裂腹鱼溃疡病病原的分离鉴定 总被引:3,自引:0,他引:3
从患溃疡病的齐口裂腹鱼(Schizothorax prenanti)的溃疡灶中分离到一高致病性菌株D060501,动物回归实验证实该菌能引起健康齐口裂腹鱼出现明显的体表溃疡症,且具有较强的致病作用。通过对分离菌进行了形态学、理化特性等较系统的表观分类学指征鉴定,该菌菌株在TSA平板上生长呈边缘光滑、有光泽的圆形白色菌落,具有溶血性。染色发现该菌为革兰氏阴性短杆菌;具运动性;能发酵葡萄糖;脂酶、氧化酶和精氨酸双水解酶反应阳性;能利用葡萄糖产气,阿拉伯糖产酸;麦芽糖、硫化氢、氰化钾和V-P反应阳性;DNA酶和尿素反应阴性。对该菌的16S rDNA进行PCR扩增、克隆及测序,得到1条长度为1 514 bp的核苷酸序列。将获得的序列在GenBank数据库中进行Blast分析并下载同源性较高的细菌序列,用DNAstar软件进行多序列比对分析并构建系统发育树,结果表明,D060501分离株与其他嗜水气单胞菌形成一个簇群,菌株D060501与嗜水气单胞菌(Genbank登录号AF468055)的亲缘关系最近,同源性高达为99.1%,由D060501菌株的系统进化树分析可见,该菌与其他7株菌在进化上距离小于1.0,结合形态及生理生化特点可将其鉴定为嗜水气单胞菌。对该菌的药敏试验表明,该菌对氯霉素和庆大霉素高度敏感,对四环素、复方新诺明、强力霉素和羧苄青霉素耐药。 相似文献
150.