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22.
SUN Yu-sheng LIN Bo WANG Si-qian LIU Yue ZHANG You-jing ZHENG Nai-rui HUANG-FU Chao-shen 《园艺学报》2014,30(1):77-84
AIM:To evaluate the effect of chronic alcohol intake on the histopathological changes of the liver and to determine the contribution of epithelial-mesenchymal transition (EMT) to hepatic fibrogenesis. METHODS:Thirty male C57BL/6 mice were randomly divided into 3 groups as following: the mice in control group was given (ig) water; the mice in low-dose alcohol group (2.0 g·kg -1·d -1) and high-dose alcohol group (4.0 g·kg -1·d -1) were given (ig) alcohol for 5 months. Alcohol-induced histopathological changes of the liver or development of hepatic fibrosis were evaluated using the histological methods with HE and Masson trichrome staining. The apoptosis of the liver was detected by TUNEL fluorometric staining (counterstained with DAPI). The activity of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) was measured by an automated biochemical analyzer. The expression of fibroblast-specific protein 1 (FSP-1), α-smooth muscle actin (α-SMA) and E-cadherin in the hepatic tissues was detected by immunofluorescence examination. The protein levels of E-cadherin, α-SMA, FSP-1, transforming growth factor β 1 (TGF-β 1) and hypoxia-inducible factor 1α (HIF-1α) were analyzed by Western blotting. RESULTS:Compared with control, the activity of serum ALT and AST, and apoptotic index of liver tissues were increased in the mice treated with alcohol for 5 months. The histopathological changes of the livers in the mice of low-dose alcohol group included steatosis and mild liver fibrosis, while severe liver fibrosis was observed in the high-dose alcohol-treated mice. Chronic alcohol consumption induced the increase in malondialdehyde (MDA) level, and the decreases in the activity of superoxide dismutase (SOD) and catalase (CAT) in the livers. It also reduced E-cadherin expression and increased α-SMA expression. FSP-1 immunostaining and albumn immunostaining positive cells were co-localized in the hepatocytes of low-dose alcohol group, but only FSP-1 positive hepatocytes were observed in high-dose alcohol group. Chronic alcohol consumption decreased E-cadherin expression and increased α-SMA, FSP-1, TGF-β 1 and HIF-1α expression in a dose-dependent manner, but the HIF-1α expression was not altered between the 2 alcohol-treated groups. CONCLUSION:Chronic alcohol intake induces the progression of hepatic fibrosis. Some fibroblasts derive from hepatocytes in liver fibrosis via EMT. The underlying mechanism is associated with the changes of the redox state, and increased TGF-β 1 generation and HIF-1α expression. 相似文献
23.
蚕丝的纤维化机理研究 总被引:4,自引:0,他引:4
本文阐明了以蚕丝蛋白的凝胶-溶胶转移和液晶态,拉伸与液晶的组合纺丝而纺成蚕丝纤维的机理。 相似文献
24.
Tomoya TAKAHASHI Akihiro MORI Hitomi ODA Isao MURAYAMA Mitsuhiko KOUNO Toshinori SAKO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):260
Changes in lipoprotein profiles occur in dairy cows during the periparturient period and in cows with transition cow disease. Here, the lipoprotein profiles of Holstein–Friesian dairy cows during the periparturient period were obtained by anion-exchange, high-performance liquid chromatography to evaluate the usefulness of lipoprotein profile evaluation during the periparturient period and in cows with fatty liver and milk fever. Lipoprotein levels (including total and high- (HDL-C) and low-density lipoprotein (LDL-C) cholesterol) in 10 healthy cows were low 4 weeks prepartum, with the lowest values at calving or within 1 week of calving; the values increased at 8 weeks postpartum. The lipoprotein levels were measured in 16 cows diagnosed with fatty liver (n=10) or milk fever (n=6) and compared to 10 healthy dairy cows. A significant difference was observed in HDL-C between healthy cows (at calving and 1 week postpartum), and the fatty liver and milk fever cows. Cows with fatty liver and milk fever had a lower mean HDL-C than the 10 healthy dairy cows at calving and 1 week postpartum. HDL-C might be a good indicator of energy balance for differentiating healthy cows from those with transition cow disease. 相似文献
25.
Responses of species to landscape modifications are generally documented through their distribution at a given time along
an intensity gradient of land transformation. By focusing on patterns, we are limited to infer ecological processes occurring
within a system and its response to environmental disturbances which can further change over time. Using diachronic datasets
at the scale of France, we analyzed the spatial responses of the black-billed magpie, which has recently colonized cities,
to landscape urbanization. This study applied recently developed statistical approaches incorporating detection uncertainty
of the magpie, based on the capture-recapture statistical framework. We tested whether, and how, extinction and colonization
mechanisms influenced variations of magpie occupancy from 2001 to 2005. In addition, we assessed the importance of the recent
urbanization of the French countryside in determining population dynamics. Overall, our analysis proved that the proportion
of urban areas and recent urbanization in France led to an increase in the probability of magpie occupancy. Unexpectedly,
the species is concomitantly disappearing from the countryside, leading to a rapid change in the distribution of the species.
This study stressed the importance of incorporating detection uncertainty in inference process about spatial dynamics. Overall,
we show how useful it is to account for the dynamic evolution of the landscape in ecological studies. 相似文献
26.
27.
过渡金属氧化物对木粉/PVC复合材料燃烧性能的影响 总被引:1,自引:0,他引:1
用锥形量热仪(CONE)和热重分析法(TGA)研究金属氧化物CuO、La2O<,3>和TiO2对木粉/PVC复合材料(WF-PVC)燃烧性能的影响.结果表明,这3种过渡金属氧化物的加入对WF-PVC均有阻燃作用.其中,CuO使热释放速率(HRR)和总热释放量(THR)降低较多,阻燃效果明显;加入金属氧化物使WF-PVC的烟释放速率(SPR)和总烟释放量(TSP)都有所降低,其中,CuO在有焰燃烧阶段烟释放量最低,抑烟效果最好.这3种氧化物都能增加成炭量.结合热重分析的结果,3种金属氧化物对WF-PVC的热降解影响是不同的.PVC和木粉之间存在相互作用,PVC显著促进了木粉的热降解,木粉的加入推迟了PVC的降解,明显提高了PVC体系的成炭量;WF-PVC的热降解行为,具有更多的PVC降解的特征. 相似文献
28.
本文简要分析不确定性因素对经济评价指标的影响,和不确定性分析的几种方法,确定项目在经济上的可靠性。 相似文献
29.
《Southern Forests》2013,75(4):373-380
The purpose of this study was to compare Beta, Gamma, Johnson's SB and Weibull functions fitted by different methods for describing the horizontal structure of Khaya ivorensis (African mahogany) plantations in Brazil. The database comprised 128 plots from six plantations at varying ages. The function fits were compared using the Kolmogoroff–Smirnoff test, mean bias and mean absolute error for the number of trees and basal area per hectare per diameter class. Johnson's SB outperformed the other functions, although all functions provided an adequate fit. The best methods were method of moments and maximum likelihood fitted using 25% of the minimum observed diameter as the location parameter for the Johnson's SB function. The errors were greater in diameter classes with higher frequencies. Location and scale parameters were highly correlated with mean diameter and age for the Weibull and Johnson's SB functions, respectively, which is convenient for diameter prediction. Gamma's scale parameter had medium correlation with age. Beta's parameters had low correlation with stand attributes assessed. 相似文献
30.
对于林火预报工作中一些不能准确预测未来或尚未发生的事件,我们可以对火灾历史资料进行统计分析,找出林火发生发展规律,应用小概率事件原理,对林火发生的可能性进行预估假设检验,从而解决问题。本文将通过实例应用来说明。 相似文献