全文获取类型
收费全文 | 2166篇 |
免费 | 85篇 |
国内免费 | 367篇 |
专业分类
林业 | 2篇 |
农学 | 35篇 |
基础科学 | 2篇 |
17篇 | |
综合类 | 552篇 |
农作物 | 1篇 |
水产渔业 | 174篇 |
畜牧兽医 | 1822篇 |
园艺 | 7篇 |
植物保护 | 6篇 |
出版年
2024年 | 6篇 |
2023年 | 20篇 |
2022年 | 50篇 |
2021年 | 65篇 |
2020年 | 66篇 |
2019年 | 83篇 |
2018年 | 39篇 |
2017年 | 49篇 |
2016年 | 82篇 |
2015年 | 85篇 |
2014年 | 121篇 |
2013年 | 89篇 |
2012年 | 172篇 |
2011年 | 184篇 |
2010年 | 114篇 |
2009年 | 134篇 |
2008年 | 126篇 |
2007年 | 196篇 |
2006年 | 153篇 |
2005年 | 129篇 |
2004年 | 112篇 |
2003年 | 75篇 |
2002年 | 73篇 |
2001年 | 74篇 |
2000年 | 73篇 |
1999年 | 35篇 |
1998年 | 32篇 |
1997年 | 38篇 |
1996年 | 17篇 |
1995年 | 24篇 |
1994年 | 24篇 |
1993年 | 13篇 |
1992年 | 17篇 |
1991年 | 10篇 |
1990年 | 8篇 |
1989年 | 10篇 |
1987年 | 4篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1983年 | 1篇 |
1981年 | 1篇 |
1979年 | 6篇 |
1978年 | 1篇 |
1976年 | 1篇 |
1973年 | 1篇 |
1956年 | 2篇 |
排序方式: 共有2618条查询结果,搜索用时 31 毫秒
91.
92.
Falcone E Cordioli P Sala G Tarantino M Tollis M 《Veterinary research communications》2001,25(2):161-167
Following the first official report of a clinically severe outbreak of bovine viral diarrhoea disease occurring in a farm in northern Italy, which had originated from the use of a live vaccine contaminated with a strain of BVD genotype II virus, a retrospective study on the prevalence of BVDV genotypes in Italy became highly relevant. For this purpose, the genotype of 78 BVDV-positive specimens, obtained in 1998–1999 from dairy cattle in an area near to where the outbreak occurred, was characterized by PCR technology. Two sets of primers, spanning the 5 UTR of BVDV genome, were used sequentially in a first round of RT-PCR, performed on viral RNA extracted directly from 15 clinical samples and 63 BVDV-infected cell-culture fluids; a second PCR assay followed to selectively amplify only BVDV genotype II. All the viruses under study were characterized as BVDV genotype I. As well as contributing to a better understanding of the prevalence of BVDV genotypes in the field, the results of the present study illustrate the possibility that novel BVDV strains can emerge in susceptible animals through the use of contaminated immunobiological products for bovine use. 相似文献
93.
Takai S Murata N Kudo R Narematsu N Kakuda T Sasaki Y Tsubaki S 《Veterinary microbiology》2001,80(4):373-381
Calves were vaccinated orally, subcutaneously or intraperitoneally with a smooth, plasmid-cured strain of Salmonella enterica serovar typhimurium, strain 81. Oral vaccination was not effective, as only 1/5 calves survived challenge with virulent S. typhimurium. Strain 81 was attenuated for calves, as only a slight rise in rectal temperatures was detected after vaccination. The organism was excreted by some calves in the faeces, but no signs of diarrhoea were observed after vaccination. After parenteral vaccination, strain 81 was able to reach the intestines, gastric associated lymphoid tissues and other internal lymphoid tissues and remained viable for up to 14 days in the bovine host. After oral challenge with a virulent strain, 9/10 vaccinated calves survived challenge as opposed to 4/10 control calves (p<0.5). Diarrhoea was present in all calves of the control groups, but in only 4/10 of the vaccinated calves. The clinical reactions of the vaccinated calves were milder than in the control calves, as the rises in rectal temperatures were lower, diarrhoea was less severe, and the challenge strain was present in fewer organs from vaccinated calves than control calves. This study showed that parenterally administered Salmonella vaccines can induce both mucosal and systemic immunity, and it is postulated that this capability of strain 81 is related to its colonisation of lymphoid tissues and other systemic and intestinal tissues. This study confirmed that plasmid-cured strains were attenuated in the bovine host and conferred significant protection after parenteral vaccination, but not oral vaccination. 相似文献
94.
在注射兔病毒性出血症 (RHD)疫苗后 ,30只兔随机分为 2组 ,试验组兔饲喂含 1 5 %黄白散的饲料。从接种到第 1 80天 ,每组取兔 8只 ,每隔 1 5d采血 1次 ,检测血凝抑制 (HI)抗体 ;在接种前 1天及接种后 5、 1 0、 1 7、 2 4、 31、 41、 5 1d ,每组取兔 1 0只 ,采血检测ANAE+淋巴细胞百分率 ;在 1 80、 2 4 0、 30 0d时 ,每组分别取兔 5只做攻毒保护试验。结果表明 :2组兔HI抗体峰值分别为 7 3log2和 9 8log2 ,差异极显著 (P <0 0 1 ) ;试验组疫苗保护期可延长 60d ;试验组ANAE+率从第 1 0天至 5 1天与对照组比较 ,差异极显著(P<0 0 1 )。所以黄白散可增强兔接种RHD疫苗后的特异性免疫功能 ,增强RHD疫苗的免疫效果。 相似文献
95.
96.
97.
98.
99.
牛病毒性腹泻/粘膜病O系弱毒冻干苗的试验生产和检验报告 总被引:5,自引:0,他引:5
1992 ̄1994年共试生产牛病毒性腹泻/粘膜病(BVD/MD)O系细胞培养弱毒冻干苗60万头份,通过实验室内安全检验、效力检验、无菌检验、水份测定、真空度检验、物理性状检验及在玉树、称多和泽库三个疫病发生严重的县试用,证明《BVD/MDO系细胞培养弱毒冻干苗制造与检验试行办法》可行,并为制定该苗的制造与检验规程提供了依据。 相似文献
100.
A Dam 《Acta veterinaria Scandinavica》1973,14(5):691-699
Experiments have been carried out with vaccination of pregnant mice against E. coli, followed by i.p. challenge of the offspring at one week of age.With a septicaemic strain the results were highly significant, and the method is therefore recommendable for testing of vaccines against such strains of E. coli.Results were less clear-cut with enteropathogenic strains of E. coli. However, with mortality rates of 40 to 45 % in baby mice born by non-vaccinated mothers and less than 15 % in baby mice born by vaccinated mothers, the difference in percentage mortality seems sufficient to warrant the use of the method also in the control of vaccines against enteropathogenic E. coli strains. 相似文献