家蚕卒倒病的病征变化与中肠组织细胞病理的研究

以不同浓度苏芸金杆菌伴孢晶体接种于４龄起蚕,观察研究家蚕细胞性中毒病的急性和慢性中毒症状及中肠组织细胞的病理变化过程及发病规律。蚕儿食下伴孢晶体６．５×１０＾－９ｗｈ ／Ｌ／Ｌ后,呈慢性中毒,可发现中肠细胞有明显的防御反应,部分蚕最终可修复受损中肠细胞导并使蚕儿恢复正常。     

A Retrospective Study of Pemoline Toxicosis in Dogs: 101 Cases (1987–1997)

Pemoline is a central nervous system stimulant commonly used for Attension Deficit Disorder in humans. This study describes the clinical syndrome associated with pemoline ingestion as well as its treatment. Ten years worth (1987–1997) of records from the ASPCa National Animal Poison Control Center involving pemoline ingestion in dogs were reviewed. The data suggests that most dogs ingesting pemoline show signs of central nervous system and cardiovascular stimulation including hyperactivity, tremors, ataxia, seizure, tachycardia, hyperthermia, and mydriasis. Blood chemistry alterations included electrolyte imbalances in some dogs. The minimum dose reported to cause clinical signs was 2.8 mg/kg and the minimum dose reported to have caused death was 10 mg/kg. Dogs generally showed clinical signs within 30 minutes to 24 hours of ingestion. The duration of clinical signs ranged from 15 hours to four days. Ninety-four percent of the dogs recovered with supportive treatment. (Vet. Emerg. & Crit. Care, 9:203–207, 1999)     

VA菌根化杉树苗抗酚能力的研究

用两株ＶＡ菌根真菌接种杉树苗,然后分别加入对羟基苯甲酸、邻香草醛和阿魏酸,结果接种ＶＡ真菌的杉树苗比未接种的长得好。接种Ｇｌｏｍｕｓ ｍｏｓｓｅａｅＺ的杉树苗干重比对照增加２７％￣８０％,接种Ｇｌｏｍｕｓ ｍｏｓｓｅａｅＷ的杉树苗干重比对照增加１０％￣６０％。     

家蚕组织中钙的亚细胞定位研究

应用锑酸盐沉淀技术研究家蚕中肠和马氏管的亚细胞钙离子分布，其螯合试验和电镜观察证明，用本试验方法产生的锑酸盐沉淀是锑酸钙，沉淀分布有规则，超微结构保存良好，是研究蚕体组织亚细胞钙离子分布较为有效的方法。作者应用这一技术观察了家蚕５龄幼虫中肠及马氏管组织的钙离子亚细胞分布，并发现氟中毒后马氏管组织的钙离子亚细胞分布有显著的变化，认为这一技术可为家蚕氟毒理研究提供一种新方法。     

Reproductive characteristics of endophyte-infected or novel tall fescue fed ewes

Reduced pregnancy rates often occur in ruminants grazing endophyte-infected (EI) tall fescue. The objectives were to characterize basal and oxytocin-induced PGF_2α concentrations in serum and reproductive function in ewes fed tall fescue seed and to determine whether addition of fish meal (FM) to a diet of EI fescue would alter PGF_2α production. Ewes were fed a diet with novel or non-toxic endophyte-infected (NE) or EI tall fescue seed containing either corn gluten meal (CG) or FM. Serum concentrations of prolactin, a measure of severity of fescue toxins, were reduced in ewes fed EI compared with NE forage seed (forage × day, P < 0.02) and were greater in NEFM than NECG-fed ewes (P < 0.03). Size and number of corpora lutea (CL), determined by trans-rectal ultrasonography, were similar between diets (P > 0.10). Serum concentrations of progesterone were reduced in ewes with two CL fed EI compared with NE seed (forage × CL number × day, P < 0.001). Oxytocin-induced PGFM concentrations during the luteal phase were determined as a measure of uterine function. On the day of oxytocin administration, peak plasma concentrations of PGFM were reduced in EI compared with NE-fed ewes (forage × time, P < 0.003), but FM did not influence PGFM concentrations. Estrous cycle length was more variable in EI than NE-fed ewes. There appears to be some asynchrony between NE and EI-fed ewes leading to changes in uterine responsiveness to oxytocin. Inclusion of FM did not alter uterine responsiveness to oxytocin.     

Benzimidazole Toxicosis in Rabbits: 13 Cases (2003 to 2011)

The objective of this study was to evaluate both clinical and histologic anomalies associated with suspected benzimidazole toxicosis in rabbits. Histopathologic records were reviewed from rabbit cases that were diagnosed with suspected benzimidazole toxicosis at 2 specialty pathology services. Medical records were also solicited from veterinarians who treated rabbits with suspected benzimidazole toxicosis. In all, 13 cases were included in this retrospective study. Histologically, presumed radiomimetic lesions of benzimidazole toxicosis were noted in 3 cases. An additional 10 cases exhibited lesions suggestive of benzimidazole toxicosis. Common clinical signs observed in the study of rabbits included inappetence, lethargy, hemorrhage, and death. One rabbit with suspected benzimidazole toxicosis survived. Benzimidazoles should be used judiciously in rabbits at published doses only after the owners are knowledgeable of the potential health risks associated with this class of drugs. The prognosis for rabbits with suspected benzimidazole toxicosis is poor, but supportive care resulted in the survival of 1 suspected case in this study.     

玉屏风散颉颃镉致鸡肝脏氧化损伤作用的研究

为了研究玉屏风散对镉对鸡肝脏氧化损伤的颉颃作用。将150只30日AA公鸡随机分为3组：对照组（A：饲喂基础日粮）、加镉组（B：组饲喂基础日粮+CdCl2 150 mg/kg）和玉屏风散加镉组（C：饲喂基础日粮+ CdCl2 150 mg/kg的基础上每天灌服玉屏风散1 mL）,所有动物自由采食,染毒后15,30,45,60天时分别从以上3组中取10只鸡剖杀,用于肝脏组织相关指标的检测。结果表明：随着镉作用时间的延长,试验组的GSH-Px、SOD的活性降低,MDA生成量增加;试验组与对照组差异显著(P<0.05)或极显著(P <0.01),C组各项指标数据介于A组和B组之间。因此,玉屏风散可减轻镉中毒对机体抗氧化功能的影响,对镉中毒有颉颃作用。     

试验性氟中毒鸡睾丸组织的病理学研究

30只180日龄京北939公鸡被随机平均分为两组，每鸡单笼饲养，饲喂相同全价配合饲料。对照组饮自来水，试验组于自来水中添加3000mg／L氟化钠。70d后测定体质量、睾丸鲜质量，并对鸡睾丸组织进行光学和超微病理学观察。结果表明，高氟可显著抑制鸡的生长和及睾丸发育，染氟鸡活体质量、睾丸质量、睾丸质量／活体质量均显著低于对照组，高氟可严重损伤鸡睾丸曲细精管、生精细胞及间质细胞。