The post-ovulatory rise in progesterone is lower and the persistence of oestrous behaviour longer during the first compared with the second cycle of the breeding season in mares

Mares are seasonally polyoestrous breeders. Therefore, the first ovulation of the season, following winter anoestrus, is the only cycle in which mares ovulate without the presence of an old CL from the previous cycle. The objective of this study was to compare the length of oestrous behaviour, and plasma progesterone concentrations during the early post-ovulatory period between mares after the first and second ovulation of the breeding season. Overall, 38 mares and 167 oestrous periods were used in the study. From those, 11 mares were used during the first and subsequent oestrous period to measure and compare the post-ovulatory rise in progesterone concentration, whereas all the mares were used to compare the length of the post-ovulatory oestrous behaviour between the first and subsequent cycles of the breeding season. The persistence of the post-ovulatory oestrus was longer (p < .001) following the first ovulation of the year (median of 52 h) compared with the subsequent ovulations (median of 36 h for second and later ovulations groups; n = 38 mares). The progesterone concentration at any of the four 8 h-intervals analysed (28, 36, 76 and 84 h post-ovulation) was lower (p < .01) following the first versus the second ovulation of the year. By 36 h post-ovulation the progesterone concentration of mares at the second ovulation of the year had passed the threshold of 2 ng/ml (2.1 ± 0.33 ng/ml), whereas in the first cycle it was 1.2 ± 0.13 ng/ml. In conclusion, mares had lower progesterone concentrations in their peripheral circulation and longer persistence of oestrous behaviour following the first ovulation of the year compared with the second and subsequent ovulatory periods of the breeding season.     

Current knowledge about the processes of luteolysis and maternal recognition of pregnancy in camelids

Camelids have many unique reproductive features that considerably differ from those of other domestic species. Females are induced ovulators with subsequent development of a corpus luteum (CL) with a short lifespan. Plasma progesterone concentration starts to increase on day 4, peaks on day 8–9 and, in non-pregnant animals, basal concentration is reached around day 10–11 post-induction of ovulation. Luteolytic pulses of prostaglandin F_2α (PGF_2α) are firstly detected on day 7 or 8 (approximately on day 5–6 after ovulation), with maximal luteolytic peaks observed between days 9 and 11 post-mating, in coincidence with a high endometrial expression of cyclooxygenase 2, a limiting enzyme in prostaglandins synthesis. Unlike other species, oxytocin seems not to be involved in the luteolytic process in these species. The CL is the main source of progesterone secretion, and its function is required to support pregnancy. Despite constant research efforts, aspects of reproduction and maternal recognition of pregnancy in camelids remain not fully understood. A transient decrease and subsequent recovery in plasma progesterone concentration are observed after day 9 post-mating in pregnant animals in association with a pulsatile release of PGF_2α and a transitory decrease in CL vascularization. Thus, embryo recognition should occur between days 8 and 12 post-mating. In camels, conceptus tissues exhibit aromatizing activity with the capacity to synthesize large amounts of oestradiol. Similarly, llama blastocysts secrete oestradiol-17β during the preimplantation stage, with a higher production during the elongation period. An increase in the endometrial expression of oestrogen receptor α is also observed on day 12 post-mating. All these evidences suggest that oestrogen could be the signal released by the embryo at the time of its recognition in camelids. Besides, nearly 98% of pregnancies are carried out in the left horn. A decrease in the endometrial expression of mucin 1 and 16 genes has been reported, suggesting that these changes are crucial for successful embryo implantation; however, no differences have been observed between horns. Thus, maternal recognition of pregnancy in camelids is a particularly complex process that must occur in a concise time to allow the rescue of the CL and embryo survival.     

超高效液相色谱-串联质谱法测定猪牛组织中甲基盐霉素残留量

建立超高效液相色谱-串联质谱法测定猪牛组织中甲基盐霉素残留量。猪、牛组织中残留的甲基盐霉素,经乙腈溶液提取,硅胶固相萃取柱净化,超高效液相色谱-串联质谱法测定,外标法定量。甲基盐霉素在5 ng/mL～250 ng/mL的浓度范围内呈现良好的线性关系,甲基盐霉素在猪、牛肌肉、肝脏、肾脏、脂肪中的检测限均为2.5μg/kg,定量限均为5.0μg/kg,猪牛肌肉、肝脏、肾脏、脂肪中5.0 ng/g～100 ng/g添加浓度范围内的回收率均值为71.9%～88.4%,批内批间RSD值均<15%。     

3-RPS并联机构奇异位形及工作空间研究

提出一种求解并联机构无奇异位形工作空间的方法。通过分析3-RPS并联机构在奇异位形时动平台的微分运动与结构约束及关节约束的关系，得到机构奇异位形判别方程和奇异位形曲面方程。探索了工作空间边界，综合分析了机构奇异位形与工作空间的关系。合理选择机构结构参数和关节变化范围，得到了无奇异位形的工作空间。     

对农村老年人口养老的主要问题分析

目前农村老年人养老现状不容乐观.家庭养老功能弱化,社区养老机制滞后,社会福利、政府救济和低保投入严重不足等,导致绝大多数农村老年人处在家庭边缘和社会保障体系之外.     

灵芝孢子受冷等静高压作用破壁提取机理的探讨

按照普通植物种子的情况,推定灵芝孢子的结构有完全充满、部分充满、有大空隙和无种仁4种形式。为此,按照这4种形式分别说明了灵芝孢子受高等静压作用时,孢子壁的破坏方式和灵芝孢子有效成分的溶出方式。     

步行轮式气垫车围裙—地面阻力特性分析

对步行轮式气垫车围裙－地面阻力特性进行了分析。建立了步行轮式气垫车围裙－地面阻力的精确数学模型，并进行了试验验证。探讨了几个主要因素对围裙－地面阻力的影响。     

黑龙江省水稻单产增长潜力预测方法研究

水稻是黑龙江省的主要粮食作物。加入WTO后,水稻生产、贸易、进出口等方面都面临新的形势,同时水稻产量的稳定和增长对于国家粮食安全、人民生活水平提高以及农民奔小康都有着重要意义。因此,探讨了应用Peal曲线函数模型对黑龙江省水稻单产进行预测的方法。