Morphologic evaluation of acute endometritis in mares with differing resistance to uterine infections

The study was designed to determine differences between normal mares and mares with endometrial pathology in the inflammatory response after bacterial challenge. Six normal mares (biopsy category I) and 4 mares with pathological endometrial changes (biopsy category II) were given an intrauterine infusion of β-hemolytic streptococci on the second day of estrus. All mares had a similar kind of inflammatory response after the bacterial inoculation as assessed by rectal and vaginal examinations. There were no significant differences in the amount of discharge, uterine tone, uterine size and cervical relaxation between the groups. Leukocytic response, as determined by endometrial smears and biopsies, was of the same magnitude in both groups. Two mares from the pathological group were not able to eliminate the infection, but had vaginal discharge and bacteriologically positive uterine swabs until the end of the experiment. It is concluded that the inability of some mares to clear uterine infections cannot be explained by a deficient inflammatory response.     

Immunohistochemical analysis of pseudorabies virus spread through neurons innervating the eyeball

Pseudorabies virus (PRV) was inoculated intraocularly into BALB/c mice, and the distribution pattern of cells positive for several neurotransmitters and viral antigens in the eyeball, trigeminal nerve ganglia, and superior cervical ganglia was examined immunohistochemically to clarify the neural route of the virus spread. In the eyeball, substance P (SP)- and calcitonin gene-related peptide (CGRP)-positive cells were detected in the ipsilateral iris and ciliary body, neuropeptide tyrosine (NPY)-positive cells were detected in the choloid membrane, and tyrosine hydroxylase (TH)-positive cells were detected in the ipsilateral inner nuclear layer of the retina; all these cells contained viral antigens. In the superior cervical ganglia, viral antigen-positive cells containing TH or NPY were found at bilateral sites. In the trigeminal nerve ganglia, viral antigen-positive cells containing SP or CGRP were found at bilateral sites. These findings indicated that the SP- and CGRP-positive ganglion cells of the trigeminal nerve ganglia innervating the iris or ciliary body, and the NPY-positive ganglion cells of the superior cervical ganglia innervating the iris, ciliary body, and choroid membrane served as the route for the virus spread. These findings also suggested that dopaminergic neurons, such as the TH-positive retinal cells and TH-positive ganglion cells of the superior cervical ganglia, served as the route for virus spread.     

灭蚤项圈对犬、猫蚤的临床试验

采用自身对照试验，以自然感染栉首蚤（Ctenocephalides sp．）的犬与猫为试验动物，验证常熟市金牧药业有限公司生产的灭蚤项圈（犬用、猫用）对蚤的杀灭与防治效果。结果表明：在整个试验期间，所有参与试验的犬、猫均未出现任何不良反应，犬、猫在挂项圈（即用药）后第14～60天，杀虫率分别在97．3％～98．4％与96．6％～98．0％之间，杀虫效果极显著（P〈o．01）。证明灭蚤项圈（犬用、猫用）对犬、猫安全可靠，能长期杀灭犬、猫蚤类。     

浅析蕉城区名优茶质量评比

本文阐述了蕉城区名优茶质量评比的参评情况并对其结果进行研究分析，提出了茶产业发展的对策。     

Paratracheal air cyst in a foal

A 1-day-old American Paint Horse was presented for a large air-filled mass along the ventral aspect of the neck. Bronchoscopy and oesophagoscopy revealed no sign of communication with the trachea or oesophagus. Radiographs and a computed tomography scan of the neck identified a communicating tract between the lumen of the cystic mass and mid-trachea. The foal was systemically healthy at this initial presentation, and delayed removal of the cyst was recommended to allow further maturation of the foal prior to undergoing general anaesthesia. Upon discharge, the cyst continued to grow in size and became more fluid than air-filled requiring repeat centesis and draining. The foal was then re-presented at 3 weeks of age for surgical removal. In surgery, direct communication with the trachea was identified and ligated. Histopathology demonstrated that the cyst lining was composed of squamous epithelium with goblet cells and occasional ciliated cells. The location, morphological features and congenital presentation of the mass were consistent with a paratracheal air cyst (PAC). Surgical resection resulted in excellent functional and cosmetic outcome. Although not previously reported in horses, PAC should be included in the differential diagnosis of an air-filled ventral neck mass in equine neonates. Complete surgical excision may result in a successful outcome.     

杜仲高产胶优良无性系的选育

以杜仲果实为研究对象,采用随机区组设计,对杜仲无性系成枝力、单枝结果数、果实含胶率和单株产果量等进行了系统分析.结果表明:不同无性系每年的单枝结果数的差异显著性都达到了极显著水平;连续2年单枝结果数及其变幅比较结果,9503、9516、9501、9532、9505、9526等无性系具有比较稳定的结果性能;不同无性系间历年单株产果量存在极显著差异;对不同无性系单株历年产果量、历年总产果量以及结果稳定性等进行综合比较,9503、9516、9532、9526等4个无性系为高产胶优良无性系.在进行杜仲高产胶优良无性系选择时,可以将连续2年单枝结果数和单株产果量的平均值作为选择指标;利用3~4年单枝结果数和单株产果量的平均值作为高产胶优良无性系早期选择的标准.     

墨西哥柏实生种子园营建及其优树子代测定的研究

通过种子园与子代测定相结合的方式,在位于滇中的石林林场建立了含102个优树(家系)的墨西哥柏实生种子园,从中探讨了墨西哥柏实生种子园的营建途径。对该种子园的52个墨西哥柏自由授粉优树子代测定的结果表明,墨西哥柏速生,10年生树高、胸径、材积、冠幅的年均生长量分别为1.04 m、1.43 cm、0.009 6m3和0.22 m。生长性状和结实性状家系间的差异达显著水平。树高、胸径、材积、冠幅、结实量的家系遗传力分别为0.76、0.66、0.68、0.74和0.65,单株遗传力分别为0.27、0.16、0.18、0.23和0.16,其生长性状和结实性状呈显著的负相关。从该种子园中评选出了9个优良家系,22个优良单株,其材积遗传增益平均为13.47%和25.2%。此外,还揭示了墨西哥柏10年生期间的生长规律。     

银杏叶用品种的初步筛选研究

通过对收集的１６个银杏优良单株４年生嫁接苗的叶产量、黄酮含量进行初步比较研究，结果表明：不同单株间叶产量、黄酮含量存在显著差异；银杏植株各器官中黄酮合量以芽最高，叶次之，根枝较低。综合分析表明：ＷＬ１６８号表现最优，其次为桂林３号等，可在生产中推广应用。     

黑银杏优良无性系选育研究

在对全县银杏种质资源普查的基础上，通过初选、复选和高接栽培对比试验，从２９７株雌银杏古树中发现并选育出了两个具有药用价值高、结实早、丰产稳产、抗逆性强等特点的黑银杏优良无性系———中银黑１号和中银黑２号。黑银杏种仁中总黄酮含量０９ｍｇ／ｋｇ，叶片中总黄酮含量８５ｇ／ｋｇ，分别比普通银杏高５０％和４１７％。文中还介绍了黑银杏的优良经济性状、生物学特性和生态学特性。