Effects of tail docking and/or teeth clipping on behavior,lesions, and physiological indicators of sows and their piglets

To evaluate effects of tail docking and/or teeth clipping on sows and their piglets, a total of 24 sows and their 302 piglets at 3 days of age were randomly allocated to one of four treatments: teeth clipping and tail docking (TCTD), teeth clipping (TC), tail docking (TD), or intact teeth and tail (Intact). Behavior of piglets and sows, lesions on the body and tail of piglets and sows' teats were inspected. Heart rates of processed piglets were increased (p < .01) during the procedures. Teeth clipping decreased body surface temperature (p < .01) of piglets during and after the procedures but tail docking did not (p > .01). Processed piglets spent more (p < .05) time lying alone and playing/fighting than sham‐processed piglets. Tail docked piglets spent less (p < .01) time standing than tail sham‐docked piglets. Intact teeth increased (p < .05) the avoidance behaviors of sows. Teeth clipping decreased (p < .05) the lesion scores on the anterior, middle, and posterior teats. Taken together, piglet teeth clipping had more impact on sows and their piglets than tail docking did in the lactation period based on our findings.     

山东省一例山羊伪狂犬病的病原分离鉴定

2017年4月,山东省某羊场饲养的山羊发生疑似伪狂犬病疫情。为确诊引发疫情的病原,采集死亡山羊组织样品,匀浆处理后接种家兔,并进行病原分离、PCR鉴定、效价测定、电镜观察及主要毒力基因测序分析。结果显示:家兔接种病料上清液后出现奇痒、麻痹,最后死亡;Marc145细胞接种病例样品后,产生变亮、变大、破裂,呈"包涵体"样等PRV特征性细胞病变,细胞分离物伪狂犬病病毒(PRV)g E PCR检测结果为阳性,病毒效价可达10-7.50 TCID50/0.1 m L,电镜观察可见病毒粒子呈圆形,有囊膜,直径约150 nm,将其命名为SDPD-17株。测序发现该毒株g E糖蛋白48、497位各插入了1个天冬氨酸,有12个氨基酸位点发生点突变;g C蛋白氨基酸序列64～70位出现了7个氨基酸(AASTPAA)插入;TK基因无碱基插入或缺失。分离鉴定结果证实,该病例由PRV野毒感染所致。     

脂肪肝鸭重要实质器官病理损伤观察

对8只脂肪肝综合征病鸭的重要内脏器官进行病理学观察。结果表明,病鸭肝脏明显肿大,色黄,质脆易碎,切面色黄、结构模糊、有明显油腻感,有的还有程度不等的瘀血、出血,表面和切面可见散在点状或斑状出血,严重时肝脏边缘部有较大范围出血。镜检发现,肝细胞普遍脂肪变性,胞浆内有数量不等的脂肪小滴,甚至整个细胞为脂肪充满,细胞核被挤压到一侧,严重者导致肝细胞坏死,肝小叶及周围有程度不等的出血。除肝脏外,其他实质器官也有程度不等变性、坏死等病变。     

Moniezia infection in the dwarf breeds of small ruminants in Southern Nigeria

A survey of Moniezia infection was conducted among two groups of sheep and goats, of dwarf breeds, in Nigeria: a. those being reared under a traditional, extensive husbandry system in groups of rural villages situated in two different ecological zones; and

b. those reared under an intensive system on experimental stations in two ecological zones of Nigeria.

Moniezia expansa was the predominant species encountered in the animals. The incidence of Moniezia infection was higher in sheep than in goats. The highest infection rates were found in kids and lambs younger than eight months old.

The clinical significance of the infection and some of the highlights of the results are discussed. It is concluded that Moniezia infection in small ruminants can pose a problem deserving of more attention and a suggestion is made for studies on the bionomics of the mite forming the intermediate host in Nigeria.     

Subchondral cyst‐like lesions in the talus of four horses

Subchondral cyst‐like lesions (SCLs) of horses have been found in various locations, but SCLs of the talus have been reported rarely. In this report, we describe 4 horses affected with a SCL of the talus. Each SCL was identified using digital radiography, and each horse was treated by intralesional injection of triamcinolone acetonide. Case details, history, findings during clinical and radiographic examination, treatment, and outcome of each horse are discussed.     

Equine Testicular Lesions Related to Invasion by Nematodes

Nematodes of the genuses Strongylus and Setaria are able to erratically migrate to the equine testis and provoke characteristic inflammatory changes. Moreover, the presence of living parasites in scrotal testes is a scarce finding in which only the tracts and related lesions may be observed. In a 20-year experience of equine testes observed in abattoirs, 13 cases of atypical inflammatory lesions in scrotal testes were collected. On opening of the vaginal cavity, hydrocele was consistantly observed, and the Morgagni's appendix was enlarged, prominent, and reddish. Large, elongated, or irregular prominent red to yellow areas were visible on the testicular or epididymal serosa. Edematous enlargement of the epididymal head or tail was occasionally observed. In two cases, the described lesions were detected together with nematodes of the species Setaria equina, free and viable or encapsulated in the visceral and parietal layers of the tunica vaginalis, confirming the hypothesized cause. Histologically, the serosal lesions were interpreted as parasitic tracts, characterized by hemorrhage and leukocyte infiltrates in recent lesions or by granulation tissue with numerous macrophages in elderly ones. In the latter, areas of squamous metaplasia could be observed in the appendix testis and in the epididymal duct. Associated microscopic lesions included diffuse periorchitis, lymphocytic interstitial orchitis with mild to severe degeneration of seminiferous tubules, and vasculitis, epididymitis, and sperm granuloma.     

CELⅠ酶的粗纯化及活性分析

TILLING（Targeting-induced local lesions in genomes）技术作为反向遗传学的一个重要研究工具，目前在众多的模式生物分子生物中得到广泛应用，其中CELⅠ酶是TILLING技术的核心。本实验通过硫酸铵沉淀、亲合层析、阴阳离子交换柱层析等常规的蛋白质纯化过程，从芹菜中粗纯化了具有较高活性的稳定的CELⅠ酶。并以杂合双链DNA为底物，对不同纯化程度的CELⅠ酶以及在不同温度、处理时间、有无Taq DNA多聚合酶的条件下进行了CELⅠ酶的错配切割活性分析。结果发现，所获得的CELⅠ酶得到了一定的纯化；CELⅠ酶的反应最适温度范围为40℃～50℃；长时间酶切特异性好；在含有Taq酶的酶切体系中，CELⅠ酶错配切割效果更佳，这与已报道的实验结果基本一致。与其他单位提供的CELⅠ酶活性相比，本实验分离纯化的CELⅠ酶活性值略高。通过对CELⅠ酶的提取及活性分析，为TILLING技术大规模和低成本的应用于家蚕功能基因组研究提供了保障。     

Application value of HPV E6/E7 mRNA and HPV E6/E7 DNA in screening cervical squamous intraepithelial lesions

AIM To investigate the diagnostic value of human papillomavirus （HPV） E6/E7 mRNA and DNA detection in cervical squamous epithelial lesion screening via retrospective analysis. METHODS The HPV E6/E7 mRNA detection, HPV E6/E7 DNA detection and p16 immunohistochemistry were performed in 206 patients undergoing ThinPrep cytology test （TCT） in Gansu Provincial Hospital of Traditional Chinese Medicine, and the results of different cytological and histological grades were observed. RESULTS The positive rates of HPV E6/E7 mRNA and DNA were increased with the progress of cervical squamous intraepithelial lesions （P<0.05） in different cytological grades and histopathological grades. The detection rate of HPV E6/E7 mRNA was lower than that of HPV E6/E7 DNA （P<0.05） in atypical squamous cells of undetermined significance （ASC-US） and low-grade squamous intraepithelial lesion （LSIL）. The results of immunohistochemistry showed that the positive rate of p16 was increased with the grade of cervical squamous epithelial lesions （P<0.05）. HPV E6/E7 DNA+p16 for cervical squamous epithelial lesion screening had the highest sensitivity, and the specificity of HPV E6/E7 mRNA was the highest. The positive predictive value of HPV E6/E7 DNA was the highest in LSIL, while the positive predictive value of HPV E6/E7 mRNA+p16 was the highest in high-grade squamous intraepithelial lesion （HSIL）. CONCLUSION HPV E6/E7 DNA+p16 has the highest sensitivity in screening for cervical squamous epithelial lesions. HPV E6/E7 mRNA more accurately reflects the activation status of HPV, especially in HSIL screening, HPV E6/E7 mRNA+p16 is more specific. The results of this research provide stronger theoretical support for clinical treatment.