基于转录组的疏花软紫草低拷贝核基因引物开发

基于高通量的转录组测序,为大量并快速的开发分子标记提供契机。本研究对紫草科植物疏花软紫草进行转录组测序,共得到92042086条reads,从疏花软紫草158446个Unigene中搜索到332个低拷贝核基因。为进行多态性验证,从中随机挑选30个设计引物,并对3个疏花软紫草种群共9个个体进行PCR扩增,结果发现其中16对引物能够扩增出单一稳定的目的条带,从中随机挑选7个片段进行群体测序并计算DNA多态性。结果表明,7对引物的多态性位点平均值为657,单倍型平均值为514,单倍型多态性 （HD） 在0389~0944之间,核苷酸多态性 （Pi） 为000148~001723。通过转录组测序技术开发的低拷贝核基因有较高的多态性,且可以有效地运用于群体遗传学和谱系地理学研究中。另外,这些低拷贝核基因能够被运用于软紫草属植物系统发育重建以及物种形成等研究工作中。     

赤峰地区敖汉苜蓿冻害及其防御技术

敖汉苜蓿在赤峰地区受冻害程度有4种类型;全株未受冻;根颈上端受冻受黑,下端仍能再生新芽;全部根颈和根的部分受冻变黑,已不能再生新芽;全株受冻死亡。引起冻害的主要原因是播种期偏晚,在播种当年生长期短,根颈入土浅。结果表明,越冬前苜蓿必须具有2个以上分枝,根颈入土深>5cm以上,根颈膨大,直径>0.3cm以上者方能减轻或避免受冻。适时早播,施种肥,延长生长期促进幼苗生长,在封冻前对平作进行浅耕覆土或深开沟磨平等措施,均能增加根颈保温层,有效提高苜蓿越冬率。     

闽东南天然降雨雨滴特征的研究

该文在实测滴谱资料的基础上建立了闽东南地区雨滴大小分布函数。F=1-exp[-(d/3.58I^0.28)^5.19I0.11]并导出了雨滴中数直径、动能与雨强的关系为D₅₀=3.34I^0.29和E=36.04I^0.29。笔者认为两型对雨滴特征是有影响的。     

涉农高校技术转移中心管理模式研究

针对农业技术的特点,农业科技成果转化有其自身的规律和特征,涉农高校技术转移管 理部门要建立推转一体化的管理模式。以安徽农业大学和江南大学的模式,有效的将横向合同 管理,公益推广体系、知识产权建设及平台搭建有机结合起来。     

Chemical changes of kenaf core binderless boards during hot pressing (I): influence of the pressing temperature condition

Self-bonding is the main factor of the performance expression of binderless boards, and therefore its clarification is considered to be an important issue. For this purpose, a series of chemical analyses were conducted on kenaf core binderless boards and their chemical changes during the hot-pressing process are discussed in this article. First of all, binderless boards were prepared from kenaf core powder at different pressing temperatures (without steam-explosion process) and were used for chemical analyses after they were reduced into powders and extracted with methanol. To investigate their chemical changes, lignin, holocellulose, and neutral sugar contents were determined, Fourier transform infrared (FTIR) spectra were recorded, and the nitrobenzene oxidation procedure was applied. As a result, it was found that parts of lignin and hemicelullose were decomposed during the hot-pressing process; however, the contribution of the resulting fractions to selfbonding was not observed. In addition, progress of condensation reactions in lignin and the formation of chemical bonds by low molecular weight conjugated carbonyl compounds in methanol extractives were observed. Thermal softening of lignin is also suggested to play an important role in the expression of board performance.     

Comparison Analysis of Immune Related Gene of Orf Virus in Sheep and Camels

The purpose of this study was to investigate the variation of Orf virus (ORFV) immune related genes after infection with different species.The ORFV genomes of sheep and camel were extracted and named ORFV-Y and ORFV-LT,respectively.Based on ORFV genome sequence published in GenBank (accession No.:KF234407.1),three pairs of specific primers were designed and synthesized to amplify the B2L,F1L and VIR gene fragments of ORFV-Y and ORFV-LT,respectively,and the amplified fragments were cloned into pMD19-T vector,transformed into E.coli DH5α competent cells.The recombinant plasmid was identified,and positive clones were selected for sequencing,DNAStar software was used to analyze the homology,amino acid sequence and phylogenetic tree of 13 ORFV genome sequences published on NCBI.The results showed that the nucleotide homology of B2L,F1L and VIR genes were 92.8% to 99.2%,95.7% to 99.5% and 77.6% to 100%,respectively.After comparing the amino acid sequence between the two genomes and the reference sequence,it was found that there were obvious differences in the immune related genes between the two genomes,and F1L gene had some rules to follow.The phylogenetic analysis of B2L,F1L and VIR genes showed that ORFV-Y was closely related to the Chinese Fujian goat strain,while ORFV-LT was far from the reference strains,and was a separate branch.The results showed that ORFV had obvious difference in immune related genes between sheep and camels,it provided a reference basis for further research on the changes of ORFV gene sequences in different species and the development of vaccines for different species in the future.     

河南省农用地集约利用与农民收入增长关系动态计量经济分析

农用地集约利用水平关系到区域粮食安全,与促进经济发展方式转变息息相关,对农用地集约利用水平进行测度并对农用地集约利用与农民收入水平进行动态研究,可以为区域土地可持续发展提供重要参考。本研究利用河南省1978—2012年的相关数据,通过构建适合河南省农用地集约利用评价的指标体系,借助BP神经网络对河南省农用地集约利用水平进行综合测度,并在此基础上采用计量经济分析考察农用地集约利用与农民收入之间的动态联系。研究表明:1978—2012年间,河南省农用地集约利用水平整体呈现上升趋势。农民收入水平与农用地集约利用指数互为Granger因果关系,农用地集约利用指数每提高1个单位,会引起农民收入提高0.628 7个单位。脉冲响应函数和方差分解结果显示,农用地集约利用水平的提高将促进农民收入的增长,且对农民收入变化的解释能力随着时间推移逐渐增强。     

人孕酮受体激素结合区基因表达产物的检测与制备

PuPR为含有编码人孕激素受体（hPR）激素结合区（LBD）基因（631-933氨基酸）的pUC19重建质拉,在其宿主Escherichiacoli（BL21）中,经1mmol/L的IPTG诱导,该转化的细菌产生一C端为6×His结尾的蛋白质,分子量45KD。该表达产物可利用Ni-NTAHRP在ng水平用Western印迹检测,并可通过亲和层析的方法,利用His－bindingresin（Ni树脂）从菌体的裂解液中方便地回收到该蛋白质。     

用最大树种数测度森林群落树种丰富度

目的 试图给出确定森林群落树种丰富度的方法,解决物种丰富度测度问题,完善森林生物多样性研究的方法。 方法 采用Monod模型表达种-面积曲线,通过数学手段确定最小森林群落面积和最大树种数。 结果 用本研究提出的方法对不同气候带森林物种丰富度进行测算,结果表明：热带森林群落具有更高的最大树种数和更大的群落最小面积,物种丰富度随气候带由热带到寒温带逐渐减少。 结论 用最大树种数能够确切表达不同气候带森林群落树种丰富度,克服了现有研究中直接把不同大小调查面积上的树种数量视为树种丰富度的弊端。     

胡麻异质型ACCase亚基基因的克隆与表达分析

为研究胡麻异质型乙酰辅酶A羧化酶BCCP、BC、α-CT和β-CT四个亚基基因的生物学功能,采用基因克隆、生物信息学和RT-PCR技术分别对胡麻accA、accB、accC和accD 4个基因进行分析。结果发现陇亚10号accA基因编码α-CT亚基,CDS序列全长为2364 bp,编码787个氨基酸;accB基因编码BCCP亚基,CDS序列全长为1173 bp,编码275个氨基酸;accC基因编码BC亚基,CDS序列全长为1574 bp,编码527个氨基酸;accD基因编码β-CT亚基,CDS序列全长为1137 bp,编码378个氨基酸,且4个基因编码的蛋白都是脂肪酸系数较高的亲水性蛋白。RT-PCR结果表明,胡麻accA、accB、accC和accD 4个基因在3个胡麻品种(高含油量的张亚2号、中含油量的陇亚10号和低含油量的R2-17)的不同时期不同组织的表达模式不同,accA、accB、accC和accD四个基因在R2-17、陇亚10号和张亚2号3个胡麻品种所有时期的不同组织中均有表达,但在种子中的表达量均明显高于其他组织,尤其在种子发育10~25 d油脂快速积累时期,4个基因的表达量都迅速增加并达到最高峰。研究表明异质型乙酰辅酶A羧化酶基因可能调控胡麻种子发育前期油脂的合成积累。