基于物联网技术的仓储香蕉成熟度智能监测与预警系统设计

为了达到对香蕉成熟度的无损检测,减少由于过度存放及处理不及时造成的损失,基于物联网技术设计并实现了一套仓储香蕉成熟度智能监测与预警系统。该系统包括智能仓储数据采集终端、手持无线中继器和后台数据服务器3个部分,智能仓储数据采集终端将采集到的仓储香蕉的温度、湿度、二氧化碳和氨气等环境信息通过手持无线中继器传输到后台数据服务器,然后基于神经网络识别算法判断给出香蕉的成熟度状态和预警信息。测试结果表明,该系统可以有效地对仓储香蕉的成熟度状态进行监测和预警,提示用户进行及时的处理,从而大大降低香蕉的损失率,具有非常高的实用价值。     

TAT-Apoptin基因原核表达载体的构建及其表达鉴定

为得到TAT-Apoptin 融合蛋白,以pMD-19T-Apoptin 为模板PCR 扩增出TATApoptin 基因,构建重组载体pET-32a-TAT-Apoptin,并对TAT-Apoptin 蛋白进行表达。PCR 和测序表明,成功构建重组载体pET-32a-TAT-Apoptin,进一步对其进行蛋白表达,发现该蛋 白能与Apoptin 多抗发生特异性结合。以上结果证明,TAT-Apoptin 基因原核表达载体构建成 功,并能在Rosetta 中进行分泌性表达。     

羊痘研究概况

羊痘病毒基因组庞大,约有150 kb,包括中间编码区和两端相同的反向末端重复序列;绵羊痘病毒和山羊痘病毒基因组彼此十分相似,约有96%的核苷酸完全相同.p32蛋白是目前世界各地分离鉴定的所有羊痘病毒株共有的且特异性很强的结构蛋白,在诊断和预防方面具有重要的应用价值.虽然羊疸从临床症状和宿主特异性上很客易做出诊断,但进一步的实验室确诊还是必要的,现已有多种检测方法和诊断试剂.控制该病最有效的方法是使用疫苗对易感动物进行免疫接种.文章从病原学、诊断和预防控制等方面对羊痘进行综述.     

基于检疫风险的植物产品动态分级管理模型初探

本文介绍了ISPM32号标准框架下基于有害生物风险的商品分类管理的总体思路和我国现行的植物检疫措施,比较了我国现行检疫措施与ISPM32号标准的区别,提出了基于检疫风险的植物产品动态分级管理模型,以期为建立适合我国国情的植物检疫分类管理体系,推进植物检疫措施规范化、标准化、国际化提供参考。     

我国家具业的发展状况与思考

介绍了我国家具业的发展，对我国家具业的市场容量、产品结构、所用材料及现有问题进行了分析和思考，并提出了解决办法。     

Root distribution pattern of the wild jack tree (Artocarpus hirsutus Lamk.) as studied by 32P soil injection method

Root distribution of the wild jack tree (Artocarpus hirsutus) was determined by selective placement of ³²P at various depths and lateral distances from the tree, in Kerala, India. In eight-and-a-half-year-old trees growing on a lateritic site, absorption of ³²P from a lateral distance of 75 cm and 30 cm depth was much greater than from 150 and 225 cm lateral distance and 60 and 90 cm depth. Root activity declined with increasing depth and lateral distance. Most of the physiologically active roots were concentrated within a radius of 75 cm and 30 cm depth, although the tap root might reach even deeper. Possibly, surface accumulation of feeder roots may cause considerable overlap of the tree and crop root zones in intercropping situations. However, as the tree roots seldom extend beyond 2.25 m laterally from the trunk, the effect of overlapping root zones and the associated competitive effects may not be a serious problem for intercropping during the first few years (<10 years after planting) of tree growth.     

羊痘病毒P32基因与羊CD58基因共表达载体的构建

为了开发出一种可行的羊痘基因疫苗,尝试用羊P32基因与CD58基因建立共表达载体.试验根据GenBank中收录的羊痘病毒(GPV)P32和羊CD58基因组序列,设计了扩增GPV P32基因和CD58基因的特异性引物,运用PCR技术从GPV中扩增出P32基因,从羊的外周血中扩增出CD58基因,并将其分别克隆到pMD18-T载体,测序正确.将P32基因去掉后端疏水区一段与CD58基因先后克隆至载体pBudCE4.1,并转化大肠埃希菌JM109,提取质粒通过酶切鉴定和测序正确,且读码框正确.说明成功获得了真核共表达质粒pBudCE4.1/CD58/P32.     

Artificial induction of maturation and fertilization in the Japanese eel, Anguilla japonica

Repeated injections of salmon pituitary extract (20 mg per fish per week) induced vitellogenesis in feminized, cultivated Japanese eels (Anguilla japonica). Oocytes were attained at the migratory nucleus stage after 11 or 12 injections. Addition of 17,20-dihydroxy-4-pregnen-3-one (DHP) into the incubation medium induced germinal vesicle breakdown (GVBD) in the oocytes at the migratory nucleus stage. An injection of DHP (2 µg g^-1 BW), given 24h after an injection of salmon pituitary extract (20 mg fish^-1), succeeded in inducing maturation and ovulation in females which contained occytes at the migratory nucleus stage. Most fish ovulated 15–18h following the DHP injection. Eggs that were ovulated within 15h after the DHP injection showed high fertility and hatchability, but eggs ovulated 18 or 21h after the DHP injection, showed considerably lower fertility and hatchability. A delay between ovulation and stripping of the eggs rapidly decreased both the fertility and hatchability within 6–9h after ovulation, indicating that artificial fertilization must be carried out immediately after ovulation. Repeated injections of human chorionic gonadotropin (hCG) at a concentration of 1 IU g^-1 BW week^-1 induced spermatogenesis, spermiation, and the acquisition of potential for sperm motility in cultivated males. Most males spermiated after the fifth or sixth injection of hCG, and the milt weight gradually increased and remained constant (1–2 g) from the 11th to 31th injection. Sperm motility peaked 24h after each weekly injection, and decreased from the 3^rd day after the injection. Potassium ions are an essential constituent for the maintenance of motility in the eel spermatozoa. Artificial seminal plasma containing 15.2 mM KCl is applicable as a milt diluent. Using these techniques developed for female and male eels, we have succeeded in obtaining many fertilized eggs from cultivated eels.     

不同臭氧浓度对水培烤烟吸收~(32)P的影响

应用3 2 P研究了不同臭氧浓度对水培烤烟吸收磷素营养的影响。结果表明 :在水培法中低浓度臭氧使根对磷素吸收量所占全植株的百分率随培植时间而减少 ,而茎则随培植时间而增加 ;高浓度臭氧则在整个培植期间根、茎的吸收变化不大。两种培植方法磷素在叶中的分布情况都是 :下叶 >中叶 >上叶。烟株各部分的吸收情况 ,根 >茎 >下叶 >中叶 >上叶 ,且两种培植方法对磷的吸收量都与培植时间呈显著正相关 ,高浓度比低浓度臭氧吸收的磷多而快     

应用^14C,^32P研究稀土对人参碳代谢及磷吸收的效应

试验结果表明,稀土对人参碳代谢和根外磷的吸收有促进作用。稀土处理后人参光合速率捉高了10.58％,参根皂甙含量增加8.32％,红果期稀土处理,人参叶面涂布磷吸收率比对照提高10.51％,参根中磷的积累增加19.58％。