Differences in the geographic distribution of lymphoma subtypes in Golden retrievers in the USA

The purpose of this cross‐sectional study was to examine differences in the geographic distribution of two distinct subtypes of canine lymphoma (CL), B‐cell lymphoma (BCL) and T‐zone lymphoma (TZL), in the USA while accounting for heritable risks associated with the outcome of disease through inclusion of only one breed of dog. This study included 454 Golden retrievers and associations between geographic areas of the USA and the phenotypic variant of lymphoma were examined using multivariable logistic regression. There was a detectable difference in the geographic distribution of BCL and TZL with dogs in the Northeast [odds ratio (OR) = 3.4, 95% confidence interval (CI) = 1.6–7.0] and East North Central regions (OR = 12.1, 95%CI = 3.6–40.5) being more likely to be diagnosed with TZL as compared to dogs in the Mountain region of the USA. The finding of non‐random geographic distribution of lymphoma subtypes suggest that environmental risk factors may contribute to the development of different types of CL.     

现代商品仔猪传代肾细胞株的培育与应用研究

细胞系是动物病毒分离培养的重要载体。本研究以现代商品化仔猪肾脏为原始材料,拟培育新的细胞系用于动物病毒的分离和培养。利用胰酶消化法和差速贴壁相结合方法,分离纯化仔猪肾上皮细胞,并在体外进行传代培养和筛选。结果显示,试验成功得到一株可以连续传代的细胞株,命名为SDPK-D,且已在体外连续传代90代。SDPK-D细胞株F33和F83代倍增时间分别为40.9和32.7 h,细胞活率分别为97.55%和98.86%,8 h细胞贴壁率分别为91.67%和97.06%。在该细胞株接种猪伪狂犬病病毒（PRV）、猪流感病毒（SIV）、猪流行性腹泻病毒（PEDV）阳性病料均出现明显的细胞病变。本研究首次针对现代商品猪培育出一株可以在体外连续传代的细胞株,并对多种动物病毒敏感,为相关动物病毒的分离培养提供了新的细胞系选择。     

猪瘟病毒石门株在ST细胞连续传代后E0基因变异分析

为了解猪瘟病毒(CSFV)石门(Shimen)株连续传代后变异情况,根据已发表的CSFV E0基因序列(AF092448.2),设计合成引物,以在ST细胞中连续传代培养的CSFV石门株细胞毒总RNA为模板,每5代通过RT-PCR方法扩增病毒的E0基因,测定其核苷酸序列和氨基酸序列,用DNA Star软件分析比较原代、5代、10代、15代、20代、25代接毒细胞中CSFV E0基因的变异性。结果显示,接种病毒第10代的病毒E0基因在630核苷酸位点出现变异,第15代病毒在632核苷酸位点出现变异。本研究通过分子生物学方法发现CSFV Shimen株在ST细胞连续传代过程中E0基因能保持遗传的稳定性。     

L型氨基酸转运载体1对奶牛乳腺中β-酪蛋白表达的影响

为研究L型氨基酸转运载体1（L type amino acid transporter 1,LAT1）对奶牛乳腺中β-酪蛋白表达的作用,本试验采用PCR技术体外扩增奶牛LAT1基因并构建LAT1真核表达载体,采用脂质体转染技术将LAT1真核表达载体转染奶牛乳腺上皮细胞,并于转染48 h后采用Western blotting技术检测LAT1过表达后乳腺上皮细胞中LAT1、4F2hc和β-酪蛋白的表达变化。荧光显微镜检测结果显示,LAT1真核表达载体成功转染奶牛乳腺上皮细胞;Western blotting检测结果显示,LAT1过表达的奶牛乳腺上皮细胞中LAT1极显著增加（P<0.01）,β-酪蛋白的表达显著升高（P<0.05）,4F2hc表达变化不显著（P>0.05）。这些结果提示LAT1对奶牛乳腺上皮细胞乳蛋白合成具有促进作用。     

柞蚕雄蛾浓缩液对肝细胞的保护和对高脂血症的治疗效果

为探讨柞蚕雄蛾浓缩液的保健和医用价值,分别就柞蚕雄蛾浓缩液对小鼠的保肝降酶作用和对半胱氨酸所致大鼠高脂血症的影响进行了实验。结果表明：该浓缩液可明显降低四氯化碳中毒小鼠血清中的谷丙转氨酶水平,因而能减少肝细胞变性、坏死的程度和范围,对受损伤的肝组织具有明显保护作用;可明显减轻肝组织脂肪样变性及降低由半胱氨酸所致高脂血症大鼠血清中的胆固醇、甘油三酯、低密度脂蛋白浓度,提高高密度脂蛋白浓度,因而对于高脂血症有良好的治疗效果。     

Resection cystoplasty of a squamous cell carcinoma in a mare

A 22‐year‐old Arabian mare was examined for evaluation of haematuria of 2 months' duration. Complete blood cell count and chemistry revealed anaemia of chronic disease (i.e. normocytic, normochromic) and hypoproteinaemia. Cystoscopy revealed a haemorrhagic mass protruding into the lumen of the urinary bladder in the apical region. The mass was surgically removed by partial cystectomy performed through a caudal ventral midline laparotomy. Histological examination of the mass confirmed the diagnosis of squamous cell carcinoma. Tumours of the equine urinary bladder are rare, and when a partial cystectomy is indicated, a caudal ventral midline laparotomy may provide adequate surgical exposure even in adult horses.     

分娩前饲喂包被氯化钙对母猪产程和死胎的影响

本试验旨在研究分娩前饲喂包被氯化钙对母猪产程和死胎的影响。选取913头妊娠109 d丹系长×大和大×长二元杂交母猪,根据体况和胎次接近的原则分成3组。对照组于妊娠109 d至分娩当天每天饲喂基础饲粮,试验Ⅰ组和试验Ⅱ组在基础饲粮的基础上分别额外饲喂25和50 g/d包被氯化钙。饲养试验从妊娠109 d到分娩当天。试验过程中每天进行粪便评分,于分娩当天记录试验母猪产程、产仔数和死胎数,并采集尿液和血液分别用于测定尿液pH和血清钙离子浓度。结果表明:1)总产仔数和分娩前饲喂包被氯化钙对母猪死胎数无显著交互作用(P>0.05);试验Ⅰ组和试验Ⅱ组死胎数分别为0.31和0.28头,显著低于对照组的0.44头(P<0.05),但试验Ⅰ组和试验Ⅱ组无显著差异(P>0.05)。2)试验Ⅰ组母猪产程相较于对照组显著缩短(P<0.05),而试验Ⅱ组产程相较于对照组无显著差异(P>0.05);3组母猪胎盘排出时间之间无显著差异(P>0.05)。3)3组母猪妊娠109 d和分娩当天尿液pH之间无显著差异(P>0.05);妊娠110 d到妊娠113 d,对照组和试验Ⅰ组尿液pH无显著差异(P>0.05),试验Ⅱ组尿液pH显著低于对照组和试验Ⅰ组(P<0.05)。与对照组相比,试验组母猪血清钙离子浓度显著提高(P<0.05),妊娠112 d和分娩当天粪便评分显著降低(P<0.05);3组母猪便秘率之间无显著差异(P>0.05)。综上所述,母猪妊娠109 d至分娩前饲喂25 g/d包被氯化钙可显著缩短产程,减少死胎,而且该效果不受母猪总产仔数的影响,该调控效果与其提高血液钙离子浓度有关。     

SRSF10对山羊肌内前体脂肪细胞分化的影响

旨在克隆山羊SRSF10基因序列,明确其生物学特性,并通过过表达和干扰手段阐明SRSF10对山羊肌内脂肪细胞分化的影响。本研究以简州大耳羊(Capra hircus)为试验对象,利用RT-PCR技术克隆山羊SRSF10基因序列,并进行生物信息学分析; 利用实时荧光定量PCR(real-time quantitative PCR,qPCR)技术研究其在成脂诱导分化不同阶段细胞中的表达水平; 转染pcDNA3.1-SRSF10过表达载体和SRSF10-siRNA至山羊肌内前体脂肪细胞并诱导分化,通过油红O和Bodipy染色法从形态学上明确其对脂滴积聚的影响; 通过qPCR方法检测脂肪细胞分化标志基因表达的变化。结果显示,获得山羊SRSF10基因序列为1 026 bp,其中CDS区552 bp,共编码183个氨基酸; SRSF10在诱导分化96 h的肌内脂肪细胞中表达量最高; 过表达和干扰山羊SRSF10分别促进和抑制了肌内脂肪细胞中的脂滴积聚; 过表达后基因SREBP1、PPARγ和C/EBPα的相对表达量极显著上调(P < 0.01),干扰后分化标志基因SREBP1、PPARγ和C/EBPα相对表达水平极显著降低(P < 0.01)。结果表明,山羊SRSF10是肌内脂肪细胞分化的正调控作用因子,并且这种调控作用可能主要通过调节SREBP1、PPARγ和C/EBPα的表达来实现。     

Genetic diversity,anti‐microbial resistance,plasmid profile and frequency of the Vi antigen in Salmonella Dublin strains isolated in Brazil

Salmonella Dublin is strongly adapted to cattle causing enteritis and/or systemic disease with high rates of mortality. However, it can be sporadically isolated from humans, usually causing serious disease, especially in patients with underlying chronic diseases. The aim of this study was to molecularly type S. Dublin strains isolated from humans and animals in Brazil to verify the diversity of these strains as well as to ascertain possible differences between strains isolated from humans and animals. Moreover, the presence of the capsular antigen Vi and the plasmid profile was characterized in addition to the anti‐microbial resistance against 15 drugs. For this reason, 113 S. Dublin strains isolated between 1983 and 2016 from humans (83) and animals (30) in Brazil were typed by PFGE and MLVA. The presence of the capsular antigen Vi was verified by PCR, and the phenotypic expression of the capsular antigen was determined serologically. Also, a plasmid analysis for each strain was carried out. The strains studied were divided into 35 different PFGE types and 89 MLVA‐types with a similarity of ≥80% and ≥17.5%, respectively. The plasmid sizes found ranged from 2 to >150 kb and none of the strains studied presented the capsular antigen Vi. Resistance or intermediate resistance was found in 23 strains (20.3%) that were resistant to ampicillin, ciprofloxacin, chloramphenicol, imipenem, nalidixic acid, piperacillin, streptomycin and/or tetracycline. The majority of the S. Dublin strains studied and isolated over a 33‐year period may descend from a common subtype that has been contaminating humans and animals in Brazil and able to cause invasive disease even in the absence of the capsular antigen. The higher diversity of resistance phenotypes in human isolates, as compared with animal strains, may be a reflection of the different anti‐microbial treatments used to control S. Dublin infections in humans in Brazil.