WJK-2型微机木材干燥监控系统的研制

该文介绍了ＷＪＫ ２型微机木材干燥监控系统的硬件结构原理和软件系统．该监控系统由ＭＣＳ ５１单片微计算机、Ｉ／Ｏ接口、传感器等组成．其结构简练，功能齐全，能对木材干燥过程中的各参数进行在线测量，并能根据所选定的工艺基准对木材干燥的全过程进行自动监测和控制，使其按最佳化的工艺进行     

杉木简单竞争指数及生长模型的研究

杉木胸径生长量随简单竞争指数的增大而减小，二者之间关系用指数函数拟合是适宜的．林分内简单竞争指数的最大值为平均值的１．５～２．１倍，最小值为０．５～０．７倍．林分株数按简单竞争指数的分布可用对数正态分布拟合，由此建立的各竞争指数级林木株数预估模型，结合单木生长模型，可对林分内各生长级的林木株数作出预估，为合理的抚育间伐提供依据．径阶平均竞争指数随直径增大而减小，二者之间关系用指数函数拟合是适宜的，相关系数均在０．９３以上，径阶平均竞争指数预估模型的建立，简化了竞争指数的测算，扩大了单木生长模型的适用范围，为建立从单木到全林分的整体生长模型提供了一条途径．     

鸡柔嫩艾美耳球虫单卵囊分离技术的构建及致病性研究

构建了一种新的鸡柔嫩艾美耳球虫单卵囊分离技术 ,并对杨陵柔嫩艾美耳球虫地理株进行了分离 ,对继代增殖的纯种卵囊进行了致病性试验。结果表明 ,该单卵囊分离技术简单易行 ,单卵囊感染成功率可达4 0 % ;该虫株对雏鸡有很强的致病性 ,经口接种 1× 10 5个孢子化卵囊 ,致死率高达 80 %。     

采用16位单片机的拖拉机电液悬挂控制系统

将原有机械式液压悬挂控制系统改装为电液式悬挂控制系统,其控制核心采用了MCS96系列16位单片机系统;对电液悬挂控制系统进行了硬件和软件设计,并在室内仿真试验台上进行了试验验证。试验结果表明,系统具有较好的调节性能,能够满足对拖拉机悬挂控制系统的静动态要求。     

刺槐数量性状的主成分分析及分类

刺槐20个数量性状主成分分析研究结果表明:前5个主成分累计贡献率达85.93％,数量性状可分为4大类群,类群内部因子是正相关,类群之间因子是负相关或不相关。根据主成分因子负荷量及其主要指标组合,至少可看出5个因子对立木的作用。采用相关系数矩阵进行分类,优于用协方差矩阵进行分类。     

浅谈钢筋混凝土框架结构设计

结合钢筋混凝土结构的应用特点、从混凝土结构中框架结构在地震作用下的结构体系到构造要求,阐明在设计混凝土结构时,不但要满足受力计算要求,更要注意抗震要求。     

Development of a core set of KASP markers for assaying genetic diversity in Brassica rapa subsp. chinensis Makino

Single‐nucleotide polymorphisms (SNPs) are rapid, economical and reliable genotyping tools. Non‐heading Chinese cabbage (Brassica rapa L. subsp. chinensis Makino) is now an economically important vegetable crop worldwide. In this study, 1,167 SNPs were evaluated for 7polymorphism among 70 representative non‐heading Chinese cabbage inbred lines using a Kompetitive Allele Specific PCR (KASP) genotyping assay. On the basis of identified polymorphisms and the results of a principal component analysis, we selected 50 core SNPs that were balanced sufficiently to provide adequate information for genetic identification. The core SNPs were used for construction of a neighbour‐joining dendrogram that separated the 70 inbred lines into four main groups and several subgroups corresponding to Caixin, Heiyebaicai, Huangxinwu, Naibaicai, Taitsai, Pak‐choi, and Wutatsai. This categorization was superior to that achieved using a dataset of 479 polymorphic SNPs. To confirm the utility of the core SNP markers in genetic identification, we tested their stability and resolution using 162 commercial hybrid cultivars. The SNPs, which represent a cost‐effective, accurate marker set for germplasm analysis and cultivar identification, are suitable for molecular marker‐assisted breeding in non‐heading Chinese cabbage.     

Combined mutations in five wheat STARCH BRANCHING ENZYME II genes improve resistant starch but affect grain yield and bread-making quality

Increases in the proportion of amylose in the starch of wheat grains result in higher levels of resistant starch, a fermentable dietary fiber associated with human health benefits. The objective of this study was to assess the effect of combined mutations in five STARCH BRANCHING ENZYME II (SBEII) genes on starch composition, grain yield and bread-making quality in two hexaploid wheat varieties. Significantly higher amylose (∼60%) and resistant starch content (10-fold) was detected in the SBEII mutants than in the wild-type controls. Mutant lines showed a significant decrease in total starch (6%), kernel weight (3%) and total grain yield (6%). Effects of the mutations in bread-making quality included increases in grain hardness, starch damage, water absorption and flour protein content; and reductions in flour extraction, farinograph development and stability times, starch viscosity, and loaf volume. Several traits showed significant interactions between genotypes, varieties, and environments, suggesting that some of the negative impacts of the combined SBEII mutations can be ameliorated by adequate selection of genetic background and growing location. The deployment of wheat varieties with increased resistant starch will likely require economic incentives to compensate growers and millers for the significant reductions detected in grain and flour yields.     

Revisiting rice breeding methods – evaluating the use of rapid generation advance (RGA) for routine rice breeding

Rice production needs to increase in the future in order to meet increasing demands. The development of new improved and higher yielding varieties more quickly will be needed to meet this demand. However, most rice breeding programmes in the world have not changed in several decades. In this article, we revisit the evidence in favour of using rapid generation advance (RGA) as a routine breeding method. We describe preliminary activities at the International Rice Research Institute (IRRI) to re-establish RGA on a large scale as the main breeding method for irrigated rice breeding. We also describe experiences from the early adoption at the Bangladesh Rice Research Institute. Evaluation of RGA breeding lines at IRRI for yield, flowering time and plant height indicated transgressive segregation for all traits. Some RGA lines were also higher yielding than the check varieties. The cost advantages of using RGA compared to the pedigree method were also empirically determined by performing an economic analysis. This indicated that RGA is several times more cost effective and advantages will be realized after 1 year even if facilities need to be built. Based on our experience, and previous independent research empirically testing the RGA method in rice, we recommend that this method should be implemented for routine rice breeding in order to improve breeding efficiency.     

Development of a kompetitive allele‐specific PCR marker for selection of the mutated Wx‐D1d allele in wheat breeding

Waxy (Wx) protein is a key enzyme for synthesis of amylose in endosperm. Amylose content in wheat grain influences the quality of end‐use products. Seven alleles have been described at the Wx‐D1 locus, but only two of them (Wx‐D1b, Wx‐D1e) were genotyped with codominant markers. The waxy wheat line K107Wx1 developed by treating ‘Kanto 107’ seeds with ethyl methanesulphonate carries the Wx‐D1d allele. However, no molecular basis supports this nomenclature. In the present study, DNA sequence analysis confirmed that a single nucleotide polymorphism in the sixth exon of Wx‐D1 changed tryptophan at position 301 into a termination codon. Based on this sequence variation, a PCR‐based KASP marker was developed to detect this point mutation using 68 BC₈F₁ plants and 297 BC₈F₂ lines derived from the cross ‘Ningmai 14’*9/K107Wx1. Combined with codominant markers for the Wx‐A1 and Wx‐B1 alleles, waxy and non‐waxy near‐isogenic lines were distinguished. The KASP marker was efficient in identifying the mutant allele and can be used to transfer waxiness to elite lines.